LOCUS BBa_K1475010 4553 bp DNA linear 29-May-2024 DEFINITION dxs+LIMS1+appY regulated by the tet promoter ACCESSION BBa_K1475010 VERSION BBa_K1475010.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 63..65 /label=SacI RBS 66..72 /label=rbs RBS 63..72 /label=BBa_J15001 CDS 79..1941 /label=dxs coding sequence CDS 79..1944 /label=BBa_K118000 misc_feature 1953..1955 /label=SacI RBS 1956..1962 /label=rbs RBS 1953..1962 /label=BBa_J15001 CDS 1969..3639 /label=(+) limonene synthase coding region misc_feature 1969..1971 /label=start misc_feature 3634..3639 /label=stop CDS 1969..3639 /label=BBa_I742110 misc_feature 3648..3650 /label=SacI RBS 3651..3657 /label=rbs RBS 3648..3657 /label=BBa_J15001 CDS 3664..4413 /label=appY coding sequence CDS 3664..4416 /label=BBa_K118001 misc_feature 63..4416 /label=BBa_K118024 stem_loop 4436..4479 /label=stem_loop misc_feature 4425..4504 /label=BBa_B0010 terminator 4425..4504 /label=BBa_B0010 stem_loop 4520..4539 /label=T7 TE misc_feature 4546..4546 /label=stop polyA_site 4540..4553 /label=polya misc_feature 4513..4553 /label=BBa_B0012 terminator 4513..4553 /label=BBa_B0012 terminator 4425..4553 /label=BBa_B0015 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agctcaagga ggtactagat gagttttgat attgccaaat acccgaccct ggcactggtc 121 gactccaccc aggagttacg actgttgccg aaagagagtt taccgaaact ctgcgacgaa 181 ctgcgccgct atttactcga cagcgtgagc cgttccagcg ggcacttcgc ctccgggctg 241 ggcacggtcg aactgaccgt ggcgctgcac tatgtctaca acaccccgtt tgaccaattg 301 atttgggatg tggggcatca ggcttatccg cataaaattt tgaccggacg ccgcgacaaa 361 atcggcacca tccgtcagaa aggcggtctg cacccgttcc cgtggcgcgg cgaaagcgaa 421 tatgacgtat taagcgtcgg gcattcatca acctccatca gtgccggaat tggtattgcg 481 gttgctgccg aaaaagaagg caaaaatcgc cgcaccgtct gtgtcattgg cgatggcgcg 541 attaccgcag gcatggcgtt tgaagcgatg aatcacgcgg gcgatatccg tcctgatatg 601 ctggtgattc tcaacgacaa tgaaatgtcg atttccgaaa atgtcggcgc gctcaacaac 661 catctggcac agctgctttc cggtaagctt tactcttcac tgcgcgaagg cgggaaaaaa 721 gttttctctg gcgtgccgcc aattaaagag ctgctcaaac gcaccgaaga acatattaaa 781 ggcatggtag tgcctggcac gttgtttgaa gagctgggct ttaactacat cggcccggtg 841 gacggtcacg atgtgctggg gcttatcacc acgctaaaga acatgcgcga cctgaaaggc 901 ccgcagttcc tgcatatcat gaccaaaaaa ggtcgtggtt atgaaccggc agaaaaagac 961 ccgatcactt tccacgccgt gcctaaattt gatccctcca gcggttgttt gccgaaaagt 1021 agcggcggtt tgccgagcta ttcaaaaatc tttggcgact ggttgtgcga aacggcagcg 1081 aaagacaaca agctgatggc gattactccg gcgatgcgtg aaggttccgg catggtcgag 1141 ttttcacgta aattcccgga tcgctacttc gacgtggcaa ttgccgagca acacgcggtg 1201 acctttgctg cgggtctggc gattggtggg tacaaaccca ttgtcgcgat ttactccact 1261 ttcctgcaac gcgcctatga tcaggtgctg catgacgtgg cgattcaaaa gcttccggtc 1321 ctgttcgcca tcgaccgcgc gggcattgtt ggtgctgacg gtcaaaccca tcagggtgct 1381 tttgatctct cttacctgcg ctgcataccg gaaatggtca ttatgacccc gagcgatgaa 1441 aacgaatgtc gccagatgct ctataccggc tatcactata acgatggccc gtcagcggtg 1501 cgctacccgc gtggcaacgc ggtcggcgtg gaactgacgc cgctggaaaa actaccaatt 1561 ggcaaaggca ttgtgaagcg tcgtggcgag aaactggcga tccttaactt tggtacgctg 1621 atgccagaag cggcgaaagt cgccgaatcg ctgaacgcca cgctggtcga tatgcgtttt 1681 gtgaaaccgc ttgatgaagc gttaattctg gaaatggccg ccagccatga agcgctggtc 1741 accgtagaag aaaacgccat tatgggcggc gcaggcagcg gcgtgaacga agtgctgatg 1801 gcccatcgta aaccagtacc cgtgctgaac attggcctgc cggacttctt tattccgcaa 1861 ggaactcagg aagaaatgcg cgccgaactc ggcctcgatg ccgctggtat ggaagccaaa 1921 atcaaggcct ggctggcata ataatactag agctcaagga ggtactagat gcgtcgttca 1981 gcaaactacc aaccttcaat ttgggaccac gattttttgc agtcattgaa tagcaactat 2041 acggatgaag catacaaaag acgagcagaa gagctgaggg gaaaagtgaa gatagcgatt 2101 aaggatgtaa tcgagcctct ggatcagttg gagctgattg ataacttgca aagacttgga 2161 ttggctcatc gttttgagac tgagattagg aacatattga ataatatcta caacaataat 2221 aaagattata attggagaaa agaaaatctg tatgcaacct cccttgagtt tagactactt 2281 agacaacatg gctatcctgt ttctcaagag gttttcaatg gttttaaaga cgaccaggga 2341 ggcttcattt gtgatgattt caagggaata ctgagcttgc atgaagcttc gtattacagc 2401 ttagaaggag aaagcatcat ggaggaggcc tggcaattta ccagcaaaca tcttaaagaa 2461 gtgatgatca gcaagaacat ggaagaggat gtatttgtag cagaacaagc gaagcgtgca 2521 ctggaactgc ctctgcattg gaaagtgcca atgttagagg caaggtggtt catacacatt 2581 tatgagagaa gagaggacaa gaaccacctt ttacttgaac tggctaagat ggagtttaac 2641 actttgcagg caatttacca ggaagaacta aaagaaattt cagggtggtg gaaggataca 2701 ggtcttggag agaaattgag ctttgcgagg aacaggttgg tagcgtcctt cttatggagc 2761 atggggatcg cgtttgagcc tcaattcgcc tattgtcgga gagtgctcac aatctcgata 2821 gccctaatta cagtgattga tgacatttat gatgtctatg gaacattgga tgaacttgag 2881 atattcactg atgctgttga gaggtgggac atcaattatg ctttgaagca ccttccgggc 2941 tatatgaaaa tgtgttttct tgcgctttac aactttgtta atgaatttgc ttattacgtt 3001 ctcaaacaac aggattttga tttgcttctg agcataaaaa atgcatggct tggcttaata 3061 caagcctact tggtggaggc gaaatggtac catagcaagt acacaccgaa actggaagaa 3121 tacttggaaa atggattggt atcaataacg ggccctttaa ttataacgat ttcatatctt 3181 tctggtacaa atccaatcat taagaaggaa ctggaatttc tggaaagtaa tccagatata 3241 gttcactggt catccaagat tttccgtctg caagatgatt tgggaacttc atcggacgag 3301 atacagagag gggatgttcc gaaatcaatc cagtgttaca tgcatgaaac tggtgcctca 3361 gaggaagttg ctcgtcaaca catcaaggat atgatgagac agatgtggaa gaaggtgaat 3421 gcatacacag ccgataaaga ctctcccttg actggaacaa ctactgagtt cctcttgaat 3481 cttgtgagaa tgtcccattt tatgtatcta catggagatg ggcatggtgt tcaaaaccaa 3541 gagactatcg atgtcggttt tacattgctt tttcagccca ttcccttgga ggacaaacac 3601 atggctttca cagcatctcc tggcaccaaa ggctaataat actagagctc aaggaggtac 3661 tagatggatt atgtttgctc cgtagttttc atctgtcaat catttgattt aattataaac 3721 aggagagtta tctcgttcaa aaaaaattca ttgtttattg taagcgacaa aattagaagg 3781 gagttaccag tatgcccctc taaactaaga attgttgata tagataagaa aacatgttta 3841 tcctttttta tcgacgtgaa taatgagctg cctggcaaat ttactcttga taagaatggc 3901 tatattgctg aagaggaacc tccattatcg cttgtttttt ctctgtttga agggattaaa 3961 atagcagact cacactccct ttggttaaaa gaaagactat gtatatcctt acttgccatg 4021 ttcaaaaaac gcgaaagtgt aaattcattt atactaacaa atataaatac atttacctgt 4081 aaaattactg gaataatcag ttttaatatt gagcggcaat ggcatttaaa agatattgcg 4141 gaattgattt atacgagtga aagtttaata aaaaaaagat taagggatga aggaacgtca 4201 tttactgaaa tattgagaga tactaggatg aggtatgcaa aaaaactcat aacttcaaac 4261 tcttattcta tcaatgtcgt agcccagaaa tgtggctata acagtacttc atatttcata 4321 tgtgcattta aagattatta tggtgtcacg ccatctcatt attttgagaa aataatcggc 4381 gtcacagatg gaataaacaa aacaattgac taataatact agagccaggc atcaaataaa 4441 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 4501 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //