LOCUS       BBa_K1475012            2081 bp    DNA     linear       11-Jun-2024
DEFINITION  GFP controlled by the lacI and plac system
ACCESSION   BBa_K1475012
VERSION     BBa_K1475012.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1195
                     /label=BBa_K1475011
     RBS             1204..1218
                     /label=RBS-1\Strong
     RBS             1211..1215
                     /label=RBS
     misc_feature    1204..1218
                     /label=BBa_B0030
     RBS             1204..1218
                     /label=BBa_B0030
     CDS             1225..1944
                     /label=GFP protein
     CDS             1225..1944
                     /label=BBa_E0040
     stem_loop       1964..2007
                     /label=stem_loop
     misc_feature    1953..2032
                     /label=BBa_B0010
     terminator      1953..2032
                     /label=BBa_B0010
     stem_loop       2048..2067
                     /label=T7 TE
     misc_feature    2074..2074
                     /label=stop
     polyA_site      2068..2081
                     /label=polya
     misc_feature    2041..2081
                     /label=BBa_B0012
     terminator      2041..2081
                     /label=BBa_B0012
     misc_feature    1204..2081
                     /label=BBa_E0840
ORIGIN
        1 atgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
       61 tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
      121 gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
      181 tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
      241 gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
      301 cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
      361 gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
      421 actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
      481 ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
      541 caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
      601 tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
      661 agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
      721 gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
      781 gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
      841 tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
      901 gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
      961 gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
     1021 gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
     1081 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
     1141 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacatacta
     1201 gagattaaag aggagaaata ctagatgcgt aaaggagaag aacttttcac tggagttgtc
     1261 ccaattcttg ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag
     1321 ggtgaaggtg atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa
     1381 ctacctgttc catggccaac acttgtcact actttcggtt atggtgttca atgctttgcg
     1441 agatacccag atcatatgaa acagcatgac tttttcaaga gtgccatgcc cgaaggttat
     1501 gtacaggaaa gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc
     1561 aagtttgaag gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa
     1621 gatggaaaca ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc
     1681 atggcagaca aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa
     1741 gatggaagcg ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct
     1801 gtccttttac cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac
     1861 gaaaagagag accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc
     1921 atggatgaac tatacaaata ataatactag agccaggcat caaataaaac gaaaggctca
     1981 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     2041 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat a
//