LOCUS       BBa_K1481001             887 bp    DNA     linear       04-May-2024
DEFINITION  his-mRFP under rhamnose promoter
ACCESSION   BBa_K1481001
VERSION     BBa_K1481001.1  
FEATURES             Location/Qualifiers
     promoter        1..176
                     /label=pRha
     CDS             177..887
                     /label=his-mRFP
     misc_feature    14..35
                     /label=CRP binding site
     misc_feature    36..84
                     /label=RhaS binding site
     misc_feature    83..88
                     /label=-35 signal
     misc_feature    106..111
                     /label=-10 signal
     misc_feature    122..176
                     /label=5'-UTR
     misc_feature    189..206
                     /label=his-tag
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aaccgttttt ttgggctaga aataattttg tttaacttta agaaggagat atacatatgg
      181 gcagcagcca tcatcatcat catcatatgg cctcctccga ggacgtcatc aaggagttca
      241 tgcgcttcaa ggtgcgcatg gagggctccg tgaacggcca cgagttcgag atcgagggcg
      301 agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg accaagggcg
      361 gccccctgcc cttcgcctgg gacatcctgt cccctcagtt ccagtacggc tccaaggcct
      421 acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc gagggcttca
      481 agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc caggactcct
      541 ccctacagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac ttcccctccg
      601 acggccccgt aatgcagaag aagaccatgg gctgggaggc ctccaccgag cggatgtacc
      661 ccgaggacgg cgccctgaag ggcgagatca agatgaggct gaagctgaag gacggcggcc
      721 actacgacgc cgaggtcaag accacctaca tggccaagaa gcccgtgcag ctgcccggcg
      781 cctacaagac cgacatcaag ctggacatca cctcccacaa cgaggactac accatcgtgg
      841 aacagtacga gcgcgccgag ggccgccact ccaccggcgc ctaataa
//