LOCUS       BBa_K1486040            2605 bp    DNA     linear       24-May-2024
DEFINITION  sfGFPN + CpxR
ACCESSION   BBa_K1486040
VERSION     BBa_K1486040.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1215..1218
                     /label=conserved
     RBS             1211..1222
                     /label=BBa_B0034
     misc_feature    1223..1864
                     /label=BBa_K1486038
     misc_feature    1865..1903
                     /label=13 amino acids linker
     misc_feature    1865..1903
                     /label=BBa_K1486037
     CDS             1904..2605
                     /label=CpxR
     misc_feature    1904..1906
                     /label=START
     misc_feature    2602..2605
                     /label=STOP
     CDS             1904..2605
                     /label=BBa_K1486000
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc aaagaggaga aaatgcgtaa aggcgaagag ctgttcactg gtgtcgtccc
     1261 tattctggtg gaactggatg gtgatgtcaa cggtcataag ttttccgtgc gtggcgaggg
     1321 tgaaggtgac gcaactaatg gtaaactgac gctgaagttc atctgtacta ctggtaaact
     1381 gccggtacct tggccgactc tggtaacgac gctgacttat ggtgttcagt gctttgctcg
     1441 ttatccggac catatgaagc agcatgactt cttcaagtcc gccatgccgg aaggctatgt
     1501 gcaggaacgc acgatttcct ttaaggatga cggcacgtac aaaacgcgtg cggaagtgaa
     1561 atttgaaggc gataccctgg taaaccgcat tgagctgaaa ggcattgact ttaaagaaga
     1621 cggcaatatc ctgggccata agctggaata caattttaac agccacaatg tttacatcac
     1681 cgccgataaa caaaaaaatg gcattaaagc gaattttaaa attcgccaca acgtggagga
     1741 tggcagcgtg cagctggctg atcactacca gcaaaacact ccaatcggtg atggtcctgt
     1801 tctgctgcca gacaatcact atctgagcac gcaaagcgtt ctgtctaaag atccgaacga
     1861 gaaaggcggt ggctctggag gtggtgggtc cggcggtggc tctatgaata aaatcctgtt
     1921 agttgatgat gaccgagagc tgacttccct attaaaggag ctgctcgaga tggaaggctt
     1981 caacgtgatt gttgcccacg atggggaaca ggcgcttgat cttctggacg acagcattga
     2041 tttacttttg cttgacgtaa tgatgccgaa gaaaaatggt atcgacacat taaaagcact
     2101 tcgccagaca caccagacgc ctgtcattat gttgacggcg cgcggcagtg aacttgatcg
     2161 cgttctcggc cttgagctgg gcgcagatga ctatctcccg aaaccgttta atgatcgtga
     2221 gctggtggca cgtattcgcg cgatcctgcg ccgttcgcac tggagcgagc aacagcaaaa
     2281 caacgacaac ggttcaccga cactggaagt tgatgcctta gtgctgaatc caggccgtca
     2341 ggaagccagc ttcgacgggc aaacgctgga gttaaccggt actgagttta ccctgctcta
     2401 tttgctggca cagcatctgg gtcaggtggt ttcccgtgaa catttaagcc aggaagtgtt
     2461 gggcaaacgc ctgacgcctt tcgaccgcgc tattgatatg cacatttcca acctgcgtcg
     2521 taaactgccg gatcgtaaag atggtcaccc gtggtttaaa accttgcgtg gtcgcggcta
     2581 tctgatggtt tctgcttcat aataa
//