LOCUS       BBa_K1486054            3293 bp    DNA     linear       28-May-2024
DEFINITION  CheY/CheZ + split rLuc
ACCESSION   BBa_K1486054
VERSION     BBa_K1486054.1  
FEATURES             Location/Qualifiers
     promoter        1080..1210
                     /label=Pbad
     promoter        1043..1114
                     /label=PC
     misc_feature    1..1079
                     /label=AraC
     promoter        1..1210
                     /label=BBa_I0500
     misc_feature    1215..1218
                     /label=conserved
     RBS             1211..1222
                     /label=BBa_B0034
     CDS             1223..1640
                     /label=BBa_K569017
     misc_feature    1641..1670
                     /label=BBa_K1486053
     misc_feature    1671..2000
                     /label=BBa_K1486019
     misc_feature    2005..2008
                     /label=conserved
     RBS             2001..2012
                     /label=BBa_B0034
     primer_bind     2013..2032
                     /label=fwd primer
     misc_feature    2013..2015
                     /label=start
     primer_bind     2636..2657
                     /label=rev primer
     misc_feature    2655..2657
                     /label=terminator
     CDS             2013..2657
                     /label=CheZ
     misc_feature    2655..2657
                     /label=TGA to TAA
     CDS             2013..2657
                     /label=BBa_K629003
     misc_feature    2658..2687
                     /label=BBa_K1486053
     misc_feature    2688..3293
                     /label=BBa_K1486020
ORIGIN
        1 ttatgacaac ttgacggcta catcattcac tttttcttca caaccggcac ggaactcgct
       61 cgggctggcc ccggtgcatt ttttaaatac ccgcgagaaa tagagttgat cgtcaaaacc
      121 aacattgcga ccgacggtgg cgataggcat ccgggtggtg ctcaaaagca gcttcgcctg
      181 gctgatacgt tggtcctcgc gccagcttaa gacgctaatc cctaactgct ggcggaaaag
      241 atgtgacaga cgcgacggcg acaagcaaac atgctgtgcg acgctggcga tatcaaaatt
      301 gctgtctgcc aggtgatcgc tgatgtactg acaagcctcg cgtacccgat tatccatcgg
      361 tggatggagc gactcgttaa tcgcttccat gcgccgcagt aacaattgct caagcagatt
      421 tatcgccagc agctccgaat agcgcccttc cccttgcccg gcgttaatga tttgcccaaa
      481 caggtcgctg aaatgcggct ggtgcgcttc atccgggcga aagaaccccg tattggcaaa
      541 tattgacggc cagttaagcc attcatgcca gtaggcgcgc ggacgaaagt aaacccactg
      601 gtgataccat tcgcgagcct ccggatgacg accgtagtga tgaatctctc ctggcgggaa
      661 cagcaaaata tcacccggtc ggcaaacaaa ttctcgtccc tgatttttca ccaccccctg
      721 accgcgaatg gtgagattga gaatataacc tttcattccc agcggtcggt cgataaaaaa
      781 atcgagataa ccgttggcct caatcggcgt taaacccgcc accagatggg cattaaacga
      841 gtatcccggc agcaggggat cattttgcgc ttcagccata cttttcatac tcccgccatt
      901 cagagaagaa accaattgtc catattgcat cagacattgc cgtcactgcg tcttttactg
      961 gctcttctcg ctaaccaaac cggtaacccc gcttattaaa agcattctgt aacaaagcgg
     1021 gaccaaagcc atgacaaaaa cgcgtaacaa aagtgtctat aatcacggca gaaaagtcca
     1081 cattgattat ttgcacggcg tcacactttg ctatgccata gcatttttat ccataagatt
     1141 agcggatcct acctgacgct ttttatcgca actctctact gtttctccat acccgttttt
     1201 ttgggctagc aaagaggaga aacacctgca aaaggagatg gcggataaag aacttaaatt
     1261 tttggttgtg gatgactttt ccaccatgcg acgcatagtg cgtaacctgc tgaaagagct
     1321 gggattcaat aatgttgagg aagcggaaga tggcgtcgac gctctcaata agttgcaggc
     1381 aggcggttat ggatttgtta tctccgactg gaacatgccc aatatggatg gcctggaatt
     1441 gctgaaaaca attcgtgcgg atggcgcgat gtcggcattg ccagtgttaa tggtgactgc
     1501 agaagcgaag aaagagaaca tcattgctgc ggcgcaagcg ggggccagtg gctatgtggt
     1561 gaagccattt accgccgcga cgctggagga aaaactcaac aaaatctttg agaaactggg
     1621 catgtccctt tttgcaggtg ggtggcggtg gctctggagg tggtgggtcc atggcttcga
     1681 aagtttatga tccagaacaa aggaaacgga tgataactgg tccgcagtgg tgggccagat
     1741 gtaaacaaat gaatgttctt gattcattta ttaattatta tgattcagaa aaacatgcag
     1801 aaaatgctgt tattttttta catggtaacg cggcctcttc ttatttatgg cgacatgttg
     1861 tgccacatat tgagccagta gcgcggtgta ttataccaga ccttattggt atgggcaaat
     1921 caggcaaatc tggtaatggt tcttataggt tacttgatca ttacaaatat cttactgcat
     1981 ggtttgaact tcttaattta aaagaggaga aaatgatgca accatcaatc aaacctgctg
     2041 acgagcattc agctggcgat atcattgcgc gcatcggcag cctgacgcgt atgctgcgcg
     2101 acagtttgcg ggaactgggg ctggatcagg ccattgccga agcggcggaa gccatccccg
     2161 atgcgcgcga tcgtttgtac tatgttgtgc agatgaccgc ccaggctgcg gagcgggcgc
     2221 tgaacagtgt tgaggcgtca caaccgcatc aggatcaaat ggagaaatca gcaaaagcgt
     2281 taacccaacg ttgggatgac tggtttgccg atccgattga ccttgccgac gcccgtgaac
     2341 tggtaacaga tacacgacaa tttctggcag atgtacccgc gcataccagc tttactaacg
     2401 cgcaactgct ggaaatcatg atggcgcagg attttcagga tctcaccggg caggtcatta
     2461 agcggatgat ggatgtcatt caggagatcg aacgccagtt gctgatggtg ctgttggaaa
     2521 acatcccgga acaggagtcg cgtccaaaac gtgaaaacca gagtttgctt aatggacctc
     2581 aggtcgatac cagcaaagcc ggtgtggtag ccagtcagga tcaggtggac gatttgttgg
     2641 atagtcttgg attttaaggt ggcggtggct ctggaggtgg tgggtcccca aagaagatca
     2701 tttttgtcgg ccatgattgg ggtgcttgtt tggcatttca ttatagctat gagcatcaag
     2761 ataagatcaa agcaatagtt cacgctgaaa gtgtagtaga tgtgattgaa tcatgggatg
     2821 aatggcctga tattgaagaa gatattgcgt tgatcaaatc tgaagaagga gaaaaaatgg
     2881 ttttggagaa taacttcttc gtggaaacca tgttgccatc aaaaatcatg agaaagttag
     2941 aaccagaaga atttgcagca tatcttgaac cattcaaaga gaaaggtgaa gttcgtcgtc
     3001 caacattatc atggcctcgt gaaatcccgt tagtaaaagg tggtaaacct gacgttgtac
     3061 aaattgttag gaattataat gcttatctac gtgcaagtga tgatttacca aaaatgttta
     3121 ttgaatcgga cccaggattc ttttccaatg ctattgttga aggtgccaag aagtttccta
     3181 atactgaatt tgtcaaagta aaaggtcttc atttttcgca agaagatgca cctgatgaaa
     3241 tgggaaaata tatcaaatcg ttcgttgagc gagttctcaa aaatgaacaa taa
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