LOCUS       BBa_K1489006            2343 bp    DNA     linear       23-May-2024
DEFINITION  OmpA-CvGal1
ACCESSION   BBa_K1489006
VERSION     BBa_K1489006.1  
FEATURES             Location/Qualifiers
     promoter        1..131
                     /label=promoter
     misc_feature    40..57
                     /label=AraI1
     misc_feature    61..78
                     /label=AraI2
     promoter        1..130
                     /label=BBa_K206000
     misc_feature    142..144
                     /label=ATG start
     misc_feature    139..144
                     /label=NdeI site
     misc_feature    597..602
                     /label=SacI site
     CDS             577..602
                     /label=Linker
     CDS             142..576
                     /label=Lpp-OmpA
     CDS             139..602
                     /label=BBa_K1489002
     misc_feature    609..611
                     /label=ATG Start
     misc_feature    2292..2294
                     /label=TGA Stop
     CDS             609..2294
                     /label=CvGal1
     CDS             609..2294
                     /label=BBa_K1489005
     stem_loop       2310..2329
                     /label=T7 TE
     misc_feature    2336..2336
                     /label=stop
     polyA_site      2330..2343
                     /label=polya
     misc_feature    2303..2343
                     /label=BBa_B0012
     terminator      2303..2343
                     /label=BBa_B0012
ORIGIN
        1 acattgatta tttgcacggc gtcacacttt gctatgccat agcaagatag tccataagat
       61 tagcggatcc tacctgacgc tttttatcgc aactctctac tgtttctcca taccgttttt
      121 ttgggctagc tactagagca tatgaaagct actaaactgg tactgggcgc ggtaatcctg
      181 ggttctactc tgctggcagg ttgctccagc aacgctaaaa tcgatcaggg aattaacccg
      241 tatgttggct ttgaaatggg ttacgactgg ttaggtcgta tgccgtacaa aggcagcgtt
      301 gaaaacggtg catacaaagc tcagggcgtt caactgaccg ctaaactggg ttacccaatc
      361 actgacgacc tggacatcta cactcgtctg ggtggcatgg tatggcgtgc agacactaaa
      421 tccaacgttt atggtaaaaa ccacgacacc ggcgtttctc cggtcttcgc tggcggtgtt
      481 gagtacgcga tcactcctga aatcgctacc cgtctggaat accagtggac caacaacatc
      541 ggtgacgcac acaccatcgg cactcgtccg gacaacggcg gaggttctgg aggagggagc
      601 tctactagat gacgtatcag atctcgaaaa tttcacagcc gttcacctcg tatattccaa
      661 atgggctgca tatcgggaaa catctgttac tccgtggccg tgtatcatac ggtaccgaag
      721 cgtttgcaat caacctccag cagaacccgg aaccttgtaa tggtgaggtc gcttttcatt
      781 tcaaccctcg gcccggtgaa cagcagtgcg tacgcaacag cttcgacggc ggcagttgga
      841 tggacgagga acgcgatcaa ccacattttc cttttgatga aggccgctct tttacccttc
      901 gtatcgaagt cgctgaggaa ggcttccgta cctatgtaaa tggcaagcca tacgtgaact
      961 tttctcatcg gcttgatctg ggcaacgtcc actatttgta cctgactgag ggtgcggagt
     1021 tctatgatat tagttatcag gatcgttata ccctgccgta caaatccgag atcccccagc
     1081 agatgaatgt agggaaagca gtccgtattc ggggcgcgag ccaggataac gatggatttt
     1141 ccgttaattt tgcctgcgac ccggataacg aaaactgcgc gtttcacttt aaccctcgcc
     1201 cgaatgaagg cgtagtcgtg cggaacgcga atctgggcgg gtggggagat gaagagcgcg
     1261 attacgaggc ggaatttccg tttcatccgt ataattattt cgatgcgatg tttatctgca
     1321 cggatgacaa atatctggtt cacgttaacg acaagtattt cacggagttc aaccatcgtg
     1381 ggggcgtgga tgctagctcc tttttcaata ttgtgggaaa tctcgatatt caggatgttg
     1441 aatatttcga gccgctggaa gatgatttcg ttaagacgat tccatcgggc ctggtgaaag
     1501 gcgatgtcct gatcttccgt ggcttcatga aacctggcgg ggatacattt tccatcaatt
     1561 tcatgaatgg ctactcggtg gaggatgata tcgccttcca tctgaatccg cgcgtcggcg
     1621 aaggtcaagt tgtaatgaac tgctgcatgg gtggcgcatg gggtgatgag gaacgggaag
     1681 atattccgag cccgctggcg gaccgtgaac cgttcgaagt gaaagttgtt gtgaagaaaa
     1741 acaagttcaa agtctatgtc aacggcaaaa aatgcatgaa atttgcagcc cggggcaatc
     1801 tggaggacat caaaggcgtg aacattaaag gcgaggctta tgtgtacgaa gtaaagctgg
     1861 aacgtaaact ggaagatagc tgggagtatc tgcctggcgg ttttcgggtg ggtggctggg
     1921 ttgtggtcca ggccatccct aagaaaggct ccgaaggttt tgcgattaat ttccgtaccg
     1981 gaagcgacga agattccgat attgcctttc atttcaatcc gcgcctccaa gaaaattgta
     2041 ccattcgcaa ctcctgcgcg ggcggggcgt ggggtggcga ggagcgcgat caaccggact
     2101 ttccatttga aaaaaaggac acatgcgaaa ttgccatcca ggcacagcca gaccgtttcg
     2161 taacctacgt taatggaaaa cgttatattg agtttaacca tcgtttaccg ttggaatcaa
     2221 ttacgtgtgt ggagttgaca ggtcgtgcgg atttctttga accgaagttc tttcatcacc
     2281 accatcatca ctgatactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt
     2341 ata
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