BBa_K1489002 1 BBa_K1489002 Lpp-OmpA-Linker (pSB1C3) 2014-10-05T11:00:00Z 2015-05-08T01:10:44Z Part sequence is from BBa_K103006, which originally comes from the E. coli genome. Outer membrane protein A (OmpA) is a common outer membrane protein found in bacteria that serves various purposes. For our purposes, the beta-barrel transmembrane domain can be utilized to anchor otherwise soluble proteins in the outer cell membrane. Lpp serves to direct the fusion protein, while a unstructured linker (GGGSGGGS) serves to separate OmpA from its cargo in order to avoid interactions between the two proteins. The expressed protein is structurally identical to BBa_K103006. This version of the biobrick has been moved into the new backbone, pSB1C3, which contains the gene for chloramphenicol resistance. This resistance gene contains an internal SacI site, making the SacI site at the end of the gene almost unusable for RE cloning. BBa_K1489003 mutagenizes this SacI site into a KasI in order to avoid this issue. false false _1869_ 0 20382 9 In stock false SacI site is kept in this version of Lpp-OmpA-Linker, is removed in BBa_K1489003. false Iowis Zhu annotation2397212 1 Lpp-OmpA range2397212 1 4 438 annotation2397211 1 Linker range2397211 1 439 464 annotation2397210 1 SacI site range2397210 1 459 464 annotation2397209 1 NdeI site range2397209 1 1 6 annotation2397208 1 ATG start range2397208 1 4 6 BBa_K1489005 1 BBa_K1489005 Crasseoterra virginica Galectin 1 (CvGal1) 2014-10-12T11:00:00Z 2015-05-08T01:10:44Z Genome of Crasseoterra virginica, optimized for E. coli expression Soluble beta-galactoside binding protein (galectin) originally from Crasseoterra virginica. Believed to expressed both intracellularly and extracellularly, where they serve to binding various types of n-acetylgalactosamines and initialize signal cascades. Believed to be a tetramer in its active form, as is true with most invertebrate galectins. false false _1869_ 0 20382 9 Not in stock false PstI site in the coding sequence had to be redesigned. false Iowis Zhu annotation2418344 1 ATG Start range2418344 1 1 3 annotation2418345 1 TGA Stop range2418345 1 1684 1686 annotation2418346 1 CvGal1 range2418346 1 1 1686 BBa_K206000 1 pBAD pBAD strong 2009-10-13T11:00:00Z 2015-05-08T01:11:23Z The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1]. Released HQ 2013 pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter. false false _307_ 0 4172 9 In stock true There were no special design considerations. false Amelia Hardjasa annotation2049254 1 AraI2 range2049254 1 61 78 annotation2049252 1 promoter range2049252 1 1 131 annotation2049253 1 AraI1 range2049253 1 40 57 BBa_K1489006 1 BBa_K1489006 OmpA-CvGal1 2014-10-15T11:00:00Z 2015-05-08T01:10:44Z CvGal1 is from the Crasseoterra virginica genome, codon optimized for E. coli expression. OmpA is from the E. coli genome. CvGal1 fused to OmpA in order to anchor it in the membrane. false false _1869_ 0 20382 9 Not in stock false Gibson overhangs were designed for Gibson assembly false Iowis Zhu component2422704 1 BBa_K1489002 component2422708 1 BBa_K1489005 component2422709 1 BBa_B0012 component2422698 1 BBa_K206000 annotation2422708 1 BBa_K1489005 range2422708 1 609 2294 annotation2422709 1 BBa_B0012 range2422709 1 2303 2343 annotation2422704 1 BBa_K1489002 range2422704 1 139 602 annotation2422698 1 BBa_K206000 range2422698 1 1 130 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation1690 1 polya range1690 1 28 41 annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1687 1 stop range1687 1 34 34 annotation1686 1 T7 TE range1686 1 8 27 BBa_K1489005_sequence 1 atgacgtatcagatctcgaaaatttcacagccgttcacctcgtatattccaaatgggctgcatatcgggaaacatctgttactccgtggccgtgtatcatacggtaccgaagcgtttgcaatcaacctccagcagaacccggaaccttgtaatggtgaggtcgcttttcatttcaaccctcggcccggtgaacagcagtgcgtacgcaacagcttcgacggcggcagttggatggacgaggaacgcgatcaaccacattttccttttgatgaaggccgctcttttacccttcgtatcgaagtcgctgaggaaggcttccgtacctatgtaaatggcaagccatacgtgaacttttctcatcggcttgatctgggcaacgtccactatttgtacctgactgagggtgcggagttctatgatattagttatcaggatcgttataccctgccgtacaaatccgagatcccccagcagatgaatgtagggaaagcagtccgtattcggggcgcgagccaggataacgatggattttccgttaattttgcctgcgacccggataacgaaaactgcgcgtttcactttaaccctcgcccgaatgaaggcgtagtcgtgcggaacgcgaatctgggcgggtggggagatgaagagcgcgattacgaggcggaatttccgtttcatccgtataattatttcgatgcgatgtttatctgcacggatgacaaatatctggttcacgttaacgacaagtatttcacggagttcaaccatcgtgggggcgtggatgctagctcctttttcaatattgtgggaaatctcgatattcaggatgttgaatatttcgagccgctggaagatgatttcgttaagacgattccatcgggcctggtgaaaggcgatgtcctgatcttccgtggcttcatgaaacctggcggggatacattttccatcaatttcatgaatggctactcggtggaggatgatatcgccttccatctgaatccgcgcgtcggcgaaggtcaagttgtaatgaactgctgcatgggtggcgcatggggtgatgaggaacgggaagatattccgagcccgctggcggaccgtgaaccgttcgaagtgaaagttgttgtgaagaaaaacaagttcaaagtctatgtcaacggcaaaaaatgcatgaaatttgcagcccggggcaatctggaggacatcaaaggcgtgaacattaaaggcgaggcttatgtgtacgaagtaaagctggaacgtaaactggaagatagctgggagtatctgcctggcggttttcgggtgggtggctgggttgtggtccaggccatccctaagaaaggctccgaaggttttgcgattaatttccgtaccggaagcgacgaagattccgatattgcctttcatttcaatccgcgcctccaagaaaattgtaccattcgcaactcctgcgcgggcggggcgtggggtggcgaggagcgcgatcaaccggactttccatttgaaaaaaaggacacatgcgaaattgccatccaggcacagccagaccgtttcgtaacctacgttaatggaaaacgttatattgagtttaaccatcgtttaccgttggaatcaattacgtgtgtggagttgacaggtcgtgcggatttctttgaaccgaagttctttcatcaccaccatcatcactga BBa_K1489006_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagcatatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctctactagatgacgtatcagatctcgaaaatttcacagccgttcacctcgtatattccaaatgggctgcatatcgggaaacatctgttactccgtggccgtgtatcatacggtaccgaagcgtttgcaatcaacctccagcagaacccggaaccttgtaatggtgaggtcgcttttcatttcaaccctcggcccggtgaacagcagtgcgtacgcaacagcttcgacggcggcagttggatggacgaggaacgcgatcaaccacattttccttttgatgaaggccgctcttttacccttcgtatcgaagtcgctgaggaaggcttccgtacctatgtaaatggcaagccatacgtgaacttttctcatcggcttgatctgggcaacgtccactatttgtacctgactgagggtgcggagttctatgatattagttatcaggatcgttataccctgccgtacaaatccgagatcccccagcagatgaatgtagggaaagcagtccgtattcggggcgcgagccaggataacgatggattttccgttaattttgcctgcgacccggataacgaaaactgcgcgtttcactttaaccctcgcccgaatgaaggcgtagtcgtgcggaacgcgaatctgggcgggtggggagatgaagagcgcgattacgaggcggaatttccgtttcatccgtataattatttcgatgcgatgtttatctgcacggatgacaaatatctggttcacgttaacgacaagtatttcacggagttcaaccatcgtgggggcgtggatgctagctcctttttcaatattgtgggaaatctcgatattcaggatgttgaatatttcgagccgctggaagatgatttcgttaagacgattccatcgggcctggtgaaaggcgatgtcctgatcttccgtggcttcatgaaacctggcggggatacattttccatcaatttcatgaatggctactcggtggaggatgatatcgccttccatctgaatccgcgcgtcggcgaaggtcaagttgtaatgaactgctgcatgggtggcgcatggggtgatgaggaacgggaagatattccgagcccgctggcggaccgtgaaccgttcgaagtgaaagttgttgtgaagaaaaacaagttcaaagtctatgtcaacggcaaaaaatgcatgaaatttgcagcccggggcaatctggaggacatcaaaggcgtgaacattaaaggcgaggcttatgtgtacgaagtaaagctggaacgtaaactggaagatagctgggagtatctgcctggcggttttcgggtgggtggctgggttgtggtccaggccatccctaagaaaggctccgaaggttttgcgattaatttccgtaccggaagcgacgaagattccgatattgcctttcatttcaatccgcgcctccaagaaaattgtaccattcgcaactcctgcgcgggcggggcgtggggtggcgaggagcgcgatcaaccggactttccatttgaaaaaaaggacacatgcgaaattgccatccaggcacagccagaccgtttcgtaacctacgttaatggaaaacgttatattgagtttaaccatcgtttaccgttggaatcaattacgtgtgtggagttgacaggtcgtgcggatttctttgaaccgaagttctttcatcaccaccatcatcactgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K206000_sequence 1 acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1489002_sequence 1 catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z