BBa_K1489002
1
BBa_K1489002
Lpp-OmpA-Linker (pSB1C3)
2014-10-05T11:00:00Z
2015-05-08T01:10:44Z
Part sequence is from BBa_K103006, which originally comes from the E. coli genome.
Outer membrane protein A (OmpA) is a common outer membrane protein found in bacteria that serves various purposes. For our purposes, the beta-barrel transmembrane domain can be utilized to anchor otherwise soluble proteins in the outer cell membrane. Lpp serves to direct the fusion protein, while a unstructured linker (GGGSGGGS) serves to separate OmpA from its cargo in order to avoid interactions between the two proteins.
The expressed protein is structurally identical to BBa_K103006. This version of the biobrick has been moved into the new backbone, pSB1C3, which contains the gene for chloramphenicol resistance. This resistance gene contains an internal SacI site, making the SacI site at the end of the gene almost unusable for RE cloning. BBa_K1489003 mutagenizes this SacI site into a KasI in order to avoid this issue.
false
false
_1869_
0
20382
9
In stock
false
SacI site is kept in this version of Lpp-OmpA-Linker, is removed in BBa_K1489003.
false
Iowis Zhu
annotation2397212
1
Lpp-OmpA
range2397212
1
4
438
annotation2397211
1
Linker
range2397211
1
439
464
annotation2397210
1
SacI site
range2397210
1
459
464
annotation2397209
1
NdeI site
range2397209
1
1
6
annotation2397208
1
ATG start
range2397208
1
4
6
BBa_K1489005
1
BBa_K1489005
Crasseoterra virginica Galectin 1 (CvGal1)
2014-10-12T11:00:00Z
2015-05-08T01:10:44Z
Genome of Crasseoterra virginica, optimized for E. coli expression
Soluble beta-galactoside binding protein (galectin) originally from Crasseoterra virginica. Believed to expressed both intracellularly and extracellularly, where they serve to binding various types of n-acetylgalactosamines and initialize signal cascades. Believed to be a tetramer in its active form, as is true with most invertebrate galectins.
false
false
_1869_
0
20382
9
Not in stock
false
PstI site in the coding sequence had to be redesigned.
false
Iowis Zhu
annotation2418344
1
ATG Start
range2418344
1
1
3
annotation2418345
1
TGA Stop
range2418345
1
1684
1686
annotation2418346
1
CvGal1
range2418346
1
1
1686
BBa_K206000
1
pBAD
pBAD strong
2009-10-13T11:00:00Z
2015-05-08T01:11:23Z
The sequence was obtained by applying all mutations that upregulated AraC binding and subsequent promoter activity listed in reference [1].
Released HQ 2013
pBAD is an <i>E.coli</i> promoter that is induced by L-arabinose. K206000 is a mutagenized pBAD promoter that is responsive to lower concentrations of arabinose than wild type (<partinfo>I13453</partinfo>) and, additionally, exhibits a higher maximum of protein expression as measured by coupling to a fluorescent reporter.
false
false
_307_
0
4172
9
In stock
true
There were no special design considerations.
false
Amelia Hardjasa
annotation2049254
1
AraI2
range2049254
1
61
78
annotation2049252
1
promoter
range2049252
1
1
131
annotation2049253
1
AraI1
range2049253
1
40
57
BBa_K1489006
1
BBa_K1489006
OmpA-CvGal1
2014-10-15T11:00:00Z
2015-05-08T01:10:44Z
CvGal1 is from the Crasseoterra virginica genome, codon optimized for E. coli expression.
OmpA is from the E. coli genome.
CvGal1 fused to OmpA in order to anchor it in the membrane.
false
false
_1869_
0
20382
9
Not in stock
false
Gibson overhangs were designed for Gibson assembly
false
Iowis Zhu
component2422704
1
BBa_K1489002
component2422708
1
BBa_K1489005
component2422709
1
BBa_B0012
component2422698
1
BBa_K206000
annotation2422708
1
BBa_K1489005
range2422708
1
609
2294
annotation2422709
1
BBa_B0012
range2422709
1
2303
2343
annotation2422704
1
BBa_K1489002
range2422704
1
139
602
annotation2422698
1
BBa_K206000
range2422698
1
1
130
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1690
1
polya
range1690
1
28
41
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1687
1
stop
range1687
1
34
34
annotation1686
1
T7 TE
range1686
1
8
27
BBa_K1489005_sequence
1
atgacgtatcagatctcgaaaatttcacagccgttcacctcgtatattccaaatgggctgcatatcgggaaacatctgttactccgtggccgtgtatcatacggtaccgaagcgtttgcaatcaacctccagcagaacccggaaccttgtaatggtgaggtcgcttttcatttcaaccctcggcccggtgaacagcagtgcgtacgcaacagcttcgacggcggcagttggatggacgaggaacgcgatcaaccacattttccttttgatgaaggccgctcttttacccttcgtatcgaagtcgctgaggaaggcttccgtacctatgtaaatggcaagccatacgtgaacttttctcatcggcttgatctgggcaacgtccactatttgtacctgactgagggtgcggagttctatgatattagttatcaggatcgttataccctgccgtacaaatccgagatcccccagcagatgaatgtagggaaagcagtccgtattcggggcgcgagccaggataacgatggattttccgttaattttgcctgcgacccggataacgaaaactgcgcgtttcactttaaccctcgcccgaatgaaggcgtagtcgtgcggaacgcgaatctgggcgggtggggagatgaagagcgcgattacgaggcggaatttccgtttcatccgtataattatttcgatgcgatgtttatctgcacggatgacaaatatctggttcacgttaacgacaagtatttcacggagttcaaccatcgtgggggcgtggatgctagctcctttttcaatattgtgggaaatctcgatattcaggatgttgaatatttcgagccgctggaagatgatttcgttaagacgattccatcgggcctggtgaaaggcgatgtcctgatcttccgtggcttcatgaaacctggcggggatacattttccatcaatttcatgaatggctactcggtggaggatgatatcgccttccatctgaatccgcgcgtcggcgaaggtcaagttgtaatgaactgctgcatgggtggcgcatggggtgatgaggaacgggaagatattccgagcccgctggcggaccgtgaaccgttcgaagtgaaagttgttgtgaagaaaaacaagttcaaagtctatgtcaacggcaaaaaatgcatgaaatttgcagcccggggcaatctggaggacatcaaaggcgtgaacattaaaggcgaggcttatgtgtacgaagtaaagctggaacgtaaactggaagatagctgggagtatctgcctggcggttttcgggtgggtggctgggttgtggtccaggccatccctaagaaaggctccgaaggttttgcgattaatttccgtaccggaagcgacgaagattccgatattgcctttcatttcaatccgcgcctccaagaaaattgtaccattcgcaactcctgcgcgggcggggcgtggggtggcgaggagcgcgatcaaccggactttccatttgaaaaaaaggacacatgcgaaattgccatccaggcacagccagaccgtttcgtaacctacgttaatggaaaacgttatattgagtttaaccatcgtttaccgttggaatcaattacgtgtgtggagttgacaggtcgtgcggatttctttgaaccgaagttctttcatcaccaccatcatcactga
BBa_K1489006_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagctactagagcatatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctctactagatgacgtatcagatctcgaaaatttcacagccgttcacctcgtatattccaaatgggctgcatatcgggaaacatctgttactccgtggccgtgtatcatacggtaccgaagcgtttgcaatcaacctccagcagaacccggaaccttgtaatggtgaggtcgcttttcatttcaaccctcggcccggtgaacagcagtgcgtacgcaacagcttcgacggcggcagttggatggacgaggaacgcgatcaaccacattttccttttgatgaaggccgctcttttacccttcgtatcgaagtcgctgaggaaggcttccgtacctatgtaaatggcaagccatacgtgaacttttctcatcggcttgatctgggcaacgtccactatttgtacctgactgagggtgcggagttctatgatattagttatcaggatcgttataccctgccgtacaaatccgagatcccccagcagatgaatgtagggaaagcagtccgtattcggggcgcgagccaggataacgatggattttccgttaattttgcctgcgacccggataacgaaaactgcgcgtttcactttaaccctcgcccgaatgaaggcgtagtcgtgcggaacgcgaatctgggcgggtggggagatgaagagcgcgattacgaggcggaatttccgtttcatccgtataattatttcgatgcgatgtttatctgcacggatgacaaatatctggttcacgttaacgacaagtatttcacggagttcaaccatcgtgggggcgtggatgctagctcctttttcaatattgtgggaaatctcgatattcaggatgttgaatatttcgagccgctggaagatgatttcgttaagacgattccatcgggcctggtgaaaggcgatgtcctgatcttccgtggcttcatgaaacctggcggggatacattttccatcaatttcatgaatggctactcggtggaggatgatatcgccttccatctgaatccgcgcgtcggcgaaggtcaagttgtaatgaactgctgcatgggtggcgcatggggtgatgaggaacgggaagatattccgagcccgctggcggaccgtgaaccgttcgaagtgaaagttgttgtgaagaaaaacaagttcaaagtctatgtcaacggcaaaaaatgcatgaaatttgcagcccggggcaatctggaggacatcaaaggcgtgaacattaaaggcgaggcttatgtgtacgaagtaaagctggaacgtaaactggaagatagctgggagtatctgcctggcggttttcgggtgggtggctgggttgtggtccaggccatccctaagaaaggctccgaaggttttgcgattaatttccgtaccggaagcgacgaagattccgatattgcctttcatttcaatccgcgcctccaagaaaattgtaccattcgcaactcctgcgcgggcggggcgtggggtggcgaggagcgcgatcaaccggactttccatttgaaaaaaaggacacatgcgaaattgccatccaggcacagccagaccgtttcgtaacctacgttaatggaaaacgttatattgagtttaaccatcgtttaccgttggaatcaattacgtgtgtggagttgacaggtcgtgcggatttctttgaaccgaagttctttcatcaccaccatcatcactgatactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K206000_sequence
1
acattgattatttgcacggcgtcacactttgctatgccatagcaagatagtccataagattagcggatcctacctgacgctttttatcgcaactctctactgtttctccataccgtttttttgggctagc
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1489002_sequence
1
catatgaaagctactaaactggtactgggcgcggtaatcctgggttctactctgctggcaggttgctccagcaacgctaaaatcgatcagggaattaacccgtatgttggctttgaaatgggttacgactggttaggtcgtatgccgtacaaaggcagcgttgaaaacggtgcatacaaagctcagggcgttcaactgaccgctaaactgggttacccaatcactgacgacctggacatctacactcgtctgggtggcatggtatggcgtgcagacactaaatccaacgtttatggtaaaaaccacgacaccggcgtttctccggtcttcgctggcggtgttgagtacgcgatcactcctgaaatcgctacccgtctggaataccagtggaccaacaacatcggtgacgcacacaccatcggcactcgtccggacaacggcggaggttctggaggagggagctc
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z