LOCUS       BBa_K1491010             949 bp    DNA     linear       29-May-2024
DEFINITION  WTLac-RBS-BFP-His
ACCESSION   BBa_K1491010
VERSION     BBa_K1491010.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    227..925
                     /label=BBa_K592100
     misc_feature    926..943
                     /label=6x His
     CDS             227..949
                     /label=BBa_K1491001
     misc_feature    209..949
                     /label=BBa_K1491000
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga gcgaactgat
      241 caaagagaac atgcacatga agctgtacat ggaaggcacc gttgacaacc accactttaa
      301 gtgcacgtct gagggtgagg gtaagccgta cgaaggcacc caaaccatgc gtatcaaagt
      361 tgtggagggc ggtccactgc cgttcgcttt tgacattctg gcgaccagct tcctgtacgg
      421 ttccaaaacg ttcattaacc atactcaggg cattccggat ttcttcaaac agagctttcc
      481 ggaaggtttc acctgggagc gtgtcaccac gtatgaagat ggtggtgtgt tgaccgccac
      541 ccaagatacc tccctgcaag atggctgtct gatctataac gtgaaaattc gtggcgtcaa
      601 ctttacgagc aatggtccgg tgatgcagaa gaaaaccctg ggttgggagg cgtttacgga
      661 aaccctgtat ccggccgatg gtggcctgga gggccgtaac gacatggcac tgaagctggt
      721 tggtggcagc catttgatcg caaatatcaa gacgacgtac cgcagcaaga aaccggcgaa
      781 aaatctgaag atgccgggtg tttactatgt cgactaccgt ctggaacgca ttaaagaagc
      841 gaataatgag acttacgtgg agcagcacga ggttgcagtc gcgcgctatt gcgacttgcc
      901 tagcaagctg ggtcataaac tgaatcatca tcaccaccat cactaataa
//