LOCUS       BBa_K1493520            1438 bp    DNA     linear       18-May-2024
DEFINITION  pRHA+LacI
ACCESSION   BBa_K1493520
VERSION     BBa_K1493520.1  
FEATURES             Location/Qualifiers
     promoter        1..122
                     /label=pRha
     promoter        1..122
                     /label=BBa_K914003
     misc_feature    135..138
                     /label=conserved
     RBS             131..142
                     /label=BBa_B0034
     misc_feature    1238..1276
                     /label=LVA
     CDS             149..1276
                     /label=lacI-LVA
     misc_feature    149..1276
                     /label=BBa_C0012
     misc_feature    1277..1301
                     /label=Help:Barcodes
     CDS             149..1276
                     /label=BBa_C0012
     stem_loop       1321..1364
                     /label=stem_loop
     misc_feature    1310..1389
                     /label=BBa_B0010
     terminator      1310..1389
                     /label=BBa_B0010
     stem_loop       1405..1424
                     /label=T7 TE
     misc_feature    1431..1431
                     /label=stop
     polyA_site      1425..1438
                     /label=polya
     misc_feature    1398..1438
                     /label=BBa_B0012
     terminator      1398..1438
                     /label=BBa_B0012
     misc_feature    131..1438
                     /label=BBa_P0412
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aatactagag aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga
      181 tgtcgcagag tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag
      241 ccacgtttct gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat
      301 tcccaaccgc gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac
      361 ctccagtctg gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga
      421 tcaactgggt gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa
      481 agcggcggtg cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct
      541 ggatgaccag gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct
      601 tgatgtctct gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg
      661 actgggcgtg gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc
      721 attaagttct gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa
      781 tcaaattcag ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca
      841 aaccatgcaa atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca
      901 gatggcgctg ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat
      961 ctcggtagtg ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac
     1021 catcaaacag gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc
     1081 tcagggccag gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac
     1141 caccctggcg cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca
     1201 gctggcacga caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc
     1261 tttagtagct taataactct gatagtgcta gtgtagatct ctactagagc caggcatcaa
     1321 ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg
     1381 aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//