LOCUS       BBa_K1493540            2239 bp    DNA     linear       18-May-2024
DEFINITION  pRHA+cIλ+LacI
ACCESSION   BBa_K1493540
VERSION     BBa_K1493540.1  
FEATURES             Location/Qualifiers
     promoter        1..122
                     /label=pRha
     promoter        1..122
                     /label=BBa_K914003
     misc_feature    135..138
                     /label=conserved
     RBS             131..142
                     /label=BBa_B0034
     CDS             152..859
                     /label=cI lambda
     misc_feature    860..892
                     /label=LVA
     misc_feature    899..923
                     /label=Help:Barcodes
     CDS             149..898
                     /label=BBa_C0051
     misc_feature    131..923
                     /label=BBa_S0103
     misc_feature    936..939
                     /label=conserved
     RBS             932..943
                     /label=BBa_B0034
     misc_feature    2039..2077
                     /label=LVA
     CDS             950..2077
                     /label=lacI-LVA
     misc_feature    950..2077
                     /label=BBa_C0012
     misc_feature    2078..2102
                     /label=Help:Barcodes
     CDS             950..2077
                     /label=BBa_C0012
     stem_loop       2122..2165
                     /label=stem_loop
     misc_feature    2111..2190
                     /label=BBa_B0010
     terminator      2111..2190
                     /label=BBa_B0010
     stem_loop       2206..2225
                     /label=T7 TE
     misc_feature    2232..2232
                     /label=stop
     polyA_site      2226..2239
                     /label=polya
     misc_feature    2199..2239
                     /label=BBa_B0012
     terminator      2199..2239
                     /label=BBa_B0012
     misc_feature    932..2239
                     /label=BBa_P0412
ORIGIN
        1 ccacaattca gcaaattgtg aacatcatca cgttcatctt tccctggttg ccaatggccc
       61 attttcctgt cagtaacgag aaggtcgcgt attcaggcgc tttttagact ggtcgtaatg
      121 aatactagag aaagaggaga aatactagat gagcacaaaa aagaaaccat taacacaaga
      181 gcagcttgag gacgcacgtc gccttaaagc aatttatgaa aaaaagaaaa atgaacttgg
      241 cttatcccag gaatctgtcg cagacaagat ggggatgggg cagtcaggcg ttggtgcttt
      301 atttaatggc atcaatgcat taaatgctta taacgccgca ttgcttgcaa aaattctcaa
      361 agttagcgtt gaagaattta gcccttcaat cgccagagaa atctacgaga tgtatgaagc
      421 ggttagtatg cagccgtcac ttagaagtga gtatgagtac cctgtttttt ctcatgttca
      481 ggcagggatg ttctcacctg agcttagaac ctttaccaaa ggtgatgcgg agagatgggt
      541 aagcacaacc aaaaaagcca gtgattctgc attctggctt gaggttgaag gtaattccat
      601 gaccgcacca acaggctcca agccaagctt tcctgacgga atgttaattc tcgttgaccc
      661 tgagcaggct gttgagccag gtgatttctg catagccaga cttgggggtg atgagtttac
      721 cttcaagaaa ctgatcaggg atagcggtca ggtgttttta caaccactaa acccacagta
      781 cccaatgatc ccatgcaatg agagttgttc cgttgtgggg aaagttatcg ctagtcagtg
      841 gcctgaagag acgtttggcg ctgcaaacga cgaaaactac gctttagtag cttaataacg
      901 ctgatagtgc tagtgtagat cgctactaga gaaagaggag aaatactaga tggtgaatgt
      961 gaaaccagta acgttatacg atgtcgcaga gtatgccggt gtctcttatc agaccgtttc
     1021 ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg cgggaaaaag tggaagcggc
     1081 gatggcggag ctgaattaca ttcccaaccg cgtggcacaa caactggcgg gcaaacagtc
     1141 gttgctgatt ggcgttgcca cctccagtct ggccctgcac gcgccgtcgc aaattgtcgc
     1201 ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg gtggtgtcga tggtagaacg
     1261 aagcggcgtc gaagcctgta aagcggcggt gcacaatctt ctcgcgcaac gcgtcagtgg
     1321 gctgatcatt aactatccgc tggatgacca ggatgccatt gctgtggaag ctgcctgcac
     1381 taatgttccg gcgttatttc ttgatgtctc tgaccagaca cccatcaaca gtattatttt
     1441 ctcccatgaa gacggtacgc gactgggcgt ggagcatctg gtcgcattgg gtcaccagca
     1501 aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg cgtctgcgtc tggctggctg
     1561 gcataaatat ctcactcgca atcaaattca gccgatagcg gaacgggaag gcgactggag
     1621 tgccatgtcc ggttttcaac aaaccatgca aatgctgaat gagggcatcg ttcccactgc
     1681 gatgctggtt gccaacgatc agatggcgct gggcgcaatg cgcgccatta ccgagtccgg
     1741 gctgcgcgtt ggtgcggata tctcggtagt gggatacgac gataccgaag acagctcatg
     1801 ttatatcccg ccgttaacca ccatcaaaca ggattttcgc ctgctggggc aaaccagcgt
     1861 ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag ggcaatcagc tgttgcccgt
     1921 ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg caaaccgcct ctccccgcgc
     1981 gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa gcgggcaggc
     2041 tgcaaacgac gaaaactacg ctttagtagc ttaataactc tgatagtgct agtgtagatc
     2101 tctactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt
     2161 tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt
     2221 gggcctttct gcgtttata
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