BBa_K1497020 1 BBa_K1497020 Naringenin sensor (FdeR) with GFP as reporter 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z d We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP fluorescence K1497020 mKate (DsRed variant) fluorescence K1497021 CFP fluorescence K1497022 You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device. false false _1877_ 0 12466 9 It's complicated false d false Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen component2398947 1 BBa_K1497019 component2398949 1 BBa_E0040 annotation2398947 1 BBa_K1497019 range2398947 1 1 1214 annotation2398949 1 BBa_E0040 range2398949 1 1221 1940 BBa_K1497019 1 BBa_K1497019 fdeR (Naringenin binding protein) and fde operon regulatory domain 2014-10-06T11:00:00Z 2015-05-08T01:10:45Z d fdeR - Naringenin binding protein We modified the naringenin biosensor from Siedler et al. by elimination of one EcoRI restriction site and by changing the native ribosomal binding site into the strong RBS Bba_B0034. Based on this reporter protein and 3 different fluorescence proteins, we designed 3 new biosensors for in vivo detection and determination of naringenin. The natural source of FdeR is Herbaspirillum seropedicae. You can use our reporter for measuring naringenin concentrations in your samples. Depending on which fluorophor you want to detect, you can use one of three biosensors: GFP K1497020 mKate (DsRed variant) K1497021 CFP K1497022 You can create your own naringenin sensor or your own naringenin dependent gene expression device as well. For these reasons use FdeR Biobrick K1497019 and clone your parts of interest (without RBS!) behind the device. false false _1877_ 0 12466 9 It's complicated true d false Sascha Hein, Niklas Hummel, Sebastian Barthel, Thomas Dohmen BBa_E0040 1 GFP green fluorescent protein derived from jellyfish Aequeora victoria wild-type GFP (SwissProt: P42212 2004-09-29T11:00:00Z 2016-01-26T02:09:38Z Released HQ 2013 GFP (mut3b) [note that this part does not have a barcode] false true _11_1_ 4206 61 7 In stock false true jcbraff annotation1934520 1 GFP protein range1934520 1 1 720 BBa_K1497019_sequence 1 ctccttgtctcctaccctgcctggcggggtgtggccgctatcggcgcgttgtcatggccaggctcaacagcggcgcatccagtgcgagtgcgccgccggcttgccgcgccagccctttctggtggggccatgcagcggatgagtgaagcttaagagtgagcttgatattgatcaaatggattgttttgattcatgatatggacggcatcaatacattgaccaccccatccgtcgtctatccgagggtcggcaagaacaagcacaacaacggagaccagcgccatgcgtttcaacaagctcgacctcaatcttctggtcgccctggatgcactgctcacggagatgagcatcagccgcgccgccgaaaagatccatctgagccagtcggccatgagcaatgccctggcgcggctgcgcgagtatttcgatgatgaattgctgatccaggtgggccggcgcatggagcccacgccgcgcgccgaggtgctcaaggatgcggtgcatgatgtgctgcggcgtatcgatggctccatcgcggcgctgccggccttcgtgccggccgagtccacgcgcgagtttcgcatctcggtttcggactttacgctctccgtcctcatcccccgggtgctggcgcgcgcgcacgccgagggcaagcacatccgctttgccctgatgccgcaggtgcaagacccgacccgctcgctggatcgggccgaggtggacctgctggtcttgccgcaggaattatgcacgcccgatcatcctgccgaagaggtcttccgcgaacggcatgtctgcgtggtctggcgcgacagtgcgctggcgcaaggcgagctgacgctggaacgctacatggcctcaggccatgtggtgatggtgccgcctggggccaatgcgtcgtcggtggaggcgtggatggccaggaagctgggctttgcgcgccgggtggaagtgaccagcttcagcttcgcttctgcgctggcgctggtacaggggacggaccgcatcgccacggtgcatgcccggctggcgcagctgctggctccgcaatggccggtggtgatcaaggagagtccgctgtcgctgggcgagatgcggcagatgatgcagtggcatcgctaccgcagcaatgatcctggcatccagtggctgcgtcgggtgtttctggagagtgcgcaggagatggatgcggcgctgccaggcatctgctgaca BBa_K1497020_sequence 1 ctccttgtctcctaccctgcctggcggggtgtggccgctatcggcgcgttgtcatggccaggctcaacagcggcgcatccagtgcgagtgcgccgccggcttgccgcgccagccctttctggtggggccatgcagcggatgagtgaagcttaagagtgagcttgatattgatcaaatggattgttttgattcatgatatggacggcatcaatacattgaccaccccatccgtcgtctatccgagggtcggcaagaacaagcacaacaacggagaccagcgccatgcgtttcaacaagctcgacctcaatcttctggtcgccctggatgcactgctcacggagatgagcatcagccgcgccgccgaaaagatccatctgagccagtcggccatgagcaatgccctggcgcggctgcgcgagtatttcgatgatgaattgctgatccaggtgggccggcgcatggagcccacgccgcgcgccgaggtgctcaaggatgcggtgcatgatgtgctgcggcgtatcgatggctccatcgcggcgctgccggccttcgtgccggccgagtccacgcgcgagtttcgcatctcggtttcggactttacgctctccgtcctcatcccccgggtgctggcgcgcgcgcacgccgagggcaagcacatccgctttgccctgatgccgcaggtgcaagacccgacccgctcgctggatcgggccgaggtggacctgctggtcttgccgcaggaattatgcacgcccgatcatcctgccgaagaggtcttccgcgaacggcatgtctgcgtggtctggcgcgacagtgcgctggcgcaaggcgagctgacgctggaacgctacatggcctcaggccatgtggtgatggtgccgcctggggccaatgcgtcgtcggtggaggcgtggatggccaggaagctgggctttgcgcgccgggtggaagtgaccagcttcagcttcgcttctgcgctggcgctggtacaggggacggaccgcatcgccacggtgcatgcccggctggcgcagctgctggctccgcaatggccggtggtgatcaaggagagtccgctgtcgctgggcgagatgcggcagatgatgcagtggcatcgctaccgcagcaatgatcctggcatccagtggctgcgtcgggtgtttctggagagtgcgcaggagatggatgcggcgctgccaggcatctgctgacatactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa BBa_E0040_sequence 1 atgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaataataa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z