LOCUS       BBa_K1499205            1391 bp    DNA     linear       27-May-2024
DEFINITION  MntH with amber stop codon with promoter for expression, no tRNA
ACCESSION   BBa_K1499205
VERSION     BBa_K1499205.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     promoter        1..74
                     /label=BBa_J13002
     CDS             81..1391
                     /label=MntH
     misc_feature    81..83
                     /label=ATG Start
     misc_feature    570..572
                     /label=TAG Amber Stop
     misc_feature    1389..1391
                     /label=TGA Stop
     CDS             81..1391
                     /label=BBa_K1499204
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggattccc gttctcccag cctgcccgac gaccgccccg
      121 acccgccaga acagcatctc gacgcgcggg ccggggcgac gctgcgcggg acggcgggac
      181 cacgcggagt gcggcgcatc ctgccgtttc tcgggccggc ggtcatcgcg tctatcgcct
      241 acatggaccc cggcaacttt gcgaccaaca tcgagggcgg tgcccgttac ggctacagcc
      301 tgctgtgggt gattctggcc gccaacctga tggcgatggt gattcagaac ctcagcgcca
      361 acctgggcat cgccagtggg cgcaacctgc ccgaactgat ccgtgagcgc tggccccggc
      421 ccctggtgtg gttttactgg attcaggccg aactcgtggc gatggcgacc gacctcgccg
      481 agtttctggg ggccgccctc gccattcaac tgctcaccgg cctgccgatg ttctggggcg
      541 cggtggtcac gggcgtcgtc accttctggt agctgaacct gcaaaagcgc ggcacccgtc
      601 cgctggaact cgcggtgggc gccttcgtgc tgatgatcgg ggtggcgtac ctcgtgcagg
      661 tcgtgctggc gcggcccgac ctcgcggcgg tgggcgcggg cttcgtgccc cgcttgcagg
      721 ggccgggcag cgcttacctc gcggtgggca tcatcggggc gacggtcatg ccacacgtca
      781 tctacctgca ctcggcgctc acgcagggac gcatacagac ggacaccact gaggaaaagc
      841 gccgcctcgt gcgcctgaac cgggtggacg tgatcgccgc gatggggctg gcgggcctga
      901 tcaacatgag catgctcgcc gtcgctgccg ccacctttca cggcaagaac gtggaaaacg
      961 cgggcgacct gaccaccgcc taccagacgc tcacgccgct gctggggccg gcggcgtcgg
     1021 tgctgttcgc cgtcgccctg ctcgcctcgg gcctgagcag ctcggcggtg ggcaccatgg
     1081 ccggcgacgt gatcatgcag ggcttcatgg gctttcacat cccgctgtgg ctgcgccgcc
     1141 tgatcaccat gctgcccgct ttcatcgtca ttctgctggg catggacccg tcgtcggtgc
     1201 tgatcttgtc gcaggtcatt ctgtgtttcg gggtgccctt cgcgctggtg ccgctgctgc
     1261 tgttcacggc gcggcgggac gtgatgggcg cactcgtgac ccggcgctcc ttcacagtga
     1321 ttggctgggt catcgcggtc atcatcatcg cgctcaacgg ctacctgctg tgggagctgc
     1381 tggggggctg a
//