LOCUS       BBa_K1499250             881 bp    DNA     linear       01-Jun-2024
DEFINITION  GFP (E0040) with two amber stop codons
ACCESSION   BBa_K1499250
VERSION     BBa_K1499250.1  
FEATURES             Location/Qualifiers
     misc_feature    62..64
                     /label=Start Codon
     misc_feature    776..781
                     /label=Double TAA Stop
     misc_feature    104..106
                     /label=TAG Amber Stop1
     misc_feature    416..418
                     /label=TAG Amber Stop2
     CDS             62..781
                     /label=GFP (E0040) Ambered
     misc_feature    1..55
                     /label=J23038
     misc_feature    782..861
                     /label=Terminator (B0010)
     promoter        1..35
                     /label=Strong promoter
     RBS             44..55
                     /label=Strong RBS
     primer_bind     862..881
                     /label=Internal amplif primer_R
ORIGIN
        1 ttgacagcta gctcagtcct aggtattgtg ctagctacta gagaaagagg agaaagacca
       61 gatgcgtaaa ggagaagaac ttttcactgg agttgtccca atttaggttg aattagatgg
      121 tgatgttaat gggcacaaat tttctgtcag tggagagggt gaaggtgatg caacatacgg
      181 aaaacttacc cttaaattta tttgcactac tggaaaacta cctgttccat ggccaacact
      241 tgtcactact ttcggttatg gtgttcaatg ctttgcgaga tacccagatc atatgaaaca
      301 gcatgacttt ttcaagagtg ccatgcccga aggttatgta caggaaagaa ctatattttt
      361 caaagatgac gggaactaca agacacgtgc tgaagtcaag tttgaaggtg atacctaggt
      421 taatagaatc gagttaaaag gtattgattt taaagaagat ggaaacattc ttggacacaa
      481 attggaatac aactataact cacacaatgt atacatcatg gcagacaaac aaaagaatgg
      541 aatcaaagtt aacttcaaaa ttagacacaa cattgaagat ggaagcgttc aactagcaga
      601 ccattatcaa caaaatactc caattggcga tggccctgtc cttttaccag acaaccatta
      661 cctgtccaca caatctgccc tttcgaaaga tcccaacgaa aagagagacc acatggtcct
      721 tcttgagttt gtaacagctg ctgggattac acatggcatg gatgaactat acaaataata
      781 accaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
      841 tgtttgtcgg tgaacgctct ccaattccaa ccgcctgaac g
//