LOCUS       BBa_K1500008            2026 bp    DNA     linear       31-May-2024
DEFINITION  IPTG-GFP mutated MutY
ACCESSION   BBa_K1500008
VERSION     BBa_K1500008.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    1..220
                     /label=BBa_J04500
     CDS             227..946
                     /label=GFP protein
     CDS             227..946
                     /label=BBa_E0040
     misc_feature    1..946
                     /label=BBa_K1500002
     RBS             955..967
                     /label=RBS-3\Weak
     RBS             961..964
                     /label=RBS
     misc_feature    955..967
                     /label=BBa_B0032
     RBS             955..967
                     /label=BBa_B0032
     misc_feature    1107..1108
                     /label=V45A
     CDS             974..2026
                     /label=MutY
     CDS             974..2026
                     /label=BBa_K1500992
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatgc gtaaaggaga
      241 agaacttttc actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca
      301 caaattttct gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa
      361 atttatttgc actactggaa aactacctgt tccatggcca acacttgtca ctactttcgg
      421 ttatggtgtt caatgctttg cgagataccc agatcatatg aaacagcatg actttttcaa
      481 gagtgccatg cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa
      541 ctacaagaca cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt
      601 aaaaggtatt gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta
      661 taactcacac aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt
      721 caaaattaga cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa
      781 tactccaatt ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc
      841 tgccctttcg aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac
      901 agctgctggg attacacatg gcatggatga actatacaaa taataatact agagtcacac
      961 aggaaagtac tagatgcaag cgtcgcaatt ttcagcccag gttctggact ggtacgataa
     1021 atacgggcga aaaactctgc cctggcaaat tgacaagacg ccctacaaag tatggctctc
     1081 agaagtgatg ttgcaacaaa ctcaggcggc gaccgttatc ccctattttg aacgctttat
     1141 ggcgcgcttc ccgacggtga ccgatctcgc caatgcgccg ctcgacgaag ttctccactt
     1201 gtggaccggg cttggctatt acgcccgcgc gcgcaatctg cataaagcgg cacaacaagt
     1261 ggcgacctta cacggcggta aattcccgga aacctttgag gaagttgcag cactgccggg
     1321 cgtcgggcgt tccaccgcag gcgcgattct ctcgctttct ctgggtaagc actttccgat
     1381 tctcgacggt aacgtcaaac gcgtgctggc gcgctgctat gctgtaagcg gctggcctgg
     1441 gaaaaaagag gtcgagaata aattatggag tttgagcgag caggtgacgc ccgcggttgg
     1501 cgtggaacgg tttaatcagg cgatgatgga tttgggtgcg atgatttgta cgcgctcgaa
     1561 accgaaatgt tcgctctgtc cgctacaaaa cggatgtatt gccgccgcca acaatagctg
     1621 ggcgctttat ccgggcaaaa aaccgaaaca gacgctgccg gagcgcaccg gctacttttt
     1681 gctattacag cacgaagatg aagtattgct ggcgcagcgt ccgccgagcg gattgtgggg
     1741 cggtttatac tgtttcccgc agtttgccga cgaagaaagt ttgcggcagt ggctggcgca
     1801 acggcagatt gctgccgata acctgacgca actgaccgcg tttcggcata ccttcagcca
     1861 tttccactta gatattgtgc ctatgtggct tcccgtgtcg tcattcaccg gctgcatgga
     1921 tgaaggcaat gcgctctggt ataacttagc gcaaccgccg tcagttggcc tagcggctcc
     1981 cgtggagcgt ttgttacagc agttacgcac tggcgcgccg gtttag
//