LOCUS BBa_K150009 3180 bp DNA linear 10-May-2024 DEFINITION ColicinE1 Producer Controlled by 3OC6HSL Receiver Device ACCESSION BBa_K150009 VERSION BBa_K150009.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 55..62 /label=BBa_K150100 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 75..80 /label=BBa_K150107 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 misc_feature 862..869 /label=BBa_K150100 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 misc_feature 950..957 /label=BBa_K150100 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 999..1006 /label=BBa_K150100 misc_feature 1007..1026 /label=LuxR/HSL promoter 1026..1031 /label=-35 promoter 1048..1053 /label=-10 misc_feature 1059..1059 /label=start misc_feature 1007..1061 /label=BBa_R0062 promoter 1007..1061 /label=BBa_R0062 misc_feature 1062..1069 /label=linkerHD04 misc_feature 1062..1069 /label=BBa_K150104 RBS 1070..1082 /label=BBa_K150005 RBS 1070..1082 /label=BBa_K150005 misc_feature 1083..1088 /label=BBa_K150008 CDS 1089..2657 /label=colE1 protein CDS 2654..2995 /label=colE1 immunity protein CDS 3043..3180 /label=kil protein misc_feature 1089..3180 /label=BBa_K150007 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt 1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ggaaacagaa 1081 ccggatccat ggaaaccgcg gtagcgtact ataaagatgg tgttccttat gatgataagg 1141 gacaggtaat tattactctt ttgaatggta ctcctgacgg gagtggctct ggcggcggag 1201 gtggaaaagg aggcagtaaa agtgaaagtt cagcagctat tcatgcaact gctaaatggt 1261 ctactgctca attaaagaaa acacaggcag agcaggctgc ccgggcaaaa gcagcagcgg 1321 aagcacaggc gaaagcaaag gcaaacaggg atgcgctgac tcagcgcctg aaggatatcg 1381 tgaatgaggc tcttcgtcac aatgcctcac gtacgccttc agcaacagag cttgctcatg 1441 ctaataatgc agctatgcag gcggaagcag agcgtttgcg ccttgcgaaa gcagaagaaa 1501 aagcccgtaa agaagcggaa gcagcagaaa aggcttttca ggaagcagaa caacgacgta 1561 aagagattga acgggagaag gctgaaacag aacgccagtt gaaactggct gaagctgaag 1621 agaaacgact ggctgcattg agtgaagaag ctaaagctgt tgagatcgcc caaaaaaaac 1681 tttctgctgc acaatctgaa gtggtgaaaa tggatggaga gattaagact ctcaattctc 1741 gtttaagctc cagtatccat gcccgtgatg cagaaatgaa aacgctcgct ggaaaacgaa 1801 atgaactggc tcaggcatcc gctaaatata aagaactgga tgagctggtc aaaaaactat 1861 caccaagagc caatgatccg cttcagaacc gtcctttttt tgaagcaacc agacgacggg 1921 ttggggccgg taagattaga gaagaaaaac aaaaacaggt aacagcatca gaaacacgta 1981 ttaaccggat aaatgctgat ataactcaga tccagaaggc tatttctcag gtcagtaata 2041 atcgtaatgc cggtatcgct cgtgttcatg aagctgaaga aaatttgaaa aaagcacaga 2101 ataatctcct taattcacag attaaggatg ctgttgatgc aacagttagc ttttatcaaa 2161 cgctgactga aaaatatggt gaaaaatatt cgaaaatggc acaggaactt gctgataagt 2221 ctaaaggtaa gaaaatcggc aatgtgaatg aagctctcgc tgcttttgaa aaatacaagg 2281 atgttttaaa taagaaattc agcaaagccg atcgtgatgc tatttttaat gcgttggcat 2341 cggtgaagta tgatgactgg gctaaacatt tagatcagtt tgccaagtac ttgaagatta 2401 cggggcatgt ttcttttgga tatgatgtgg tatctgatat cctaaaaatt aaggatacag 2461 gtgactggaa gccactattt cttacattag agaagaaagc agcagatgca ggggtgagtt 2521 atgttgttgc tttacttttt agcttgcttg ctggaactac attaggtatt tggggtattg 2581 ctattgttac aggtattcta tgctcctata ttgataagaa taaacttaat actataaatg 2641 aggtgttagg gatttaatta ttctttattg atataaaaag tcctagcaat ccaaatggga 2701 ttgctaggac caaacaaagt agattatata gcataaatag gtttaatttt gctacggggg 2761 cgttatttag gttttttctt ctttcgaaaa aatctttctt tatgaagtta aaagctatgt 2821 attcaatagc atattttgaa tatggacata gaatagtgct tatcactatt gcatatagca 2881 tcttatctga cacaaggaaa taataccctt cgctgttttt tgttataagg tatatatata 2941 taagtgtgca gtacaggcca aataaaatat tttttatgta gtatcttaag ctcataaatt 3001 aaacctcgcc atatattctt ttcattttat aaggatcgag ttatgaggaa aagatttttt 3061 gtgggaatat tcgcgataaa cctccttgtt ggatgtcagg ctaactatat acgtgatgtt 3121 cagggaggga ccatcgcacc atcctcctct tctaaactga cggggatcgc ggttcagtgg //