BBa_R0062
1
lux pR
Promoter (luxR & HSL regulated -- lux pR)
2003-01-31T12:00:00Z
2015-05-08T01:14:15Z
<em>V. fischeri</em>
Released HQ 2013
Promoter activated by LuxR in concert with HSL</p> <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the LuxR activator protein complexed with the autoinducer, 3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a complex with two molecules of the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
false
true
_1_
0
24
7
In stock
false
<P> <P>This promoter is based on the <em>Vibrio fischeri </em>quorum sensing gene promoters. Two genes LuxI and LuxR and transcribed in opposite directions as shown below. The original sequence from which the parts <bb_part>BBa_R0062</bb_part> and <bb_part>BBa_R0063</bb_part> were derived is shown in the picture below. <p><img src="<bb_file>Image1.gif</bb_file>" width="614" height="362"><P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation2048
1
start
range2048
1
53
53
annotation2046
1
-35
range2046
1
20
25
annotation7070
1
BBa_R0062
range7070
1
1
55
annotation2047
1
-10
range2047
1
42
47
annotation2045
1
LuxR/HSL
range2045
1
1
20
BBa_K150005
1
BBa_K150005
ribosome binding site of pTrc99a
2008-10-20T11:00:00Z
2015-05-08T01:10:46Z
-
ribosome binding site of pTrc99a
false
false
_234_
0
3299
9
Not in stock
false
-
false
Pascal Kraemer
annotation1983190
1
BBa_K150005
range1983190
1
1
13
BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation4184
1
stem_loop
range4184
1
12
55
annotation7018
1
BBa_B0010
range7018
1
1
80
BBa_K150104
1
BBa_K150104
linkerHD04
2008-10-20T11:00:00Z
2015-05-08T01:10:47Z
-
DNA linker region between lux pR promoter BBa_R0062 and ribosome binding site pTrc99a (BBa_) for the ColicinE1 Producer Controlled by 3OC6HSL Receiver Device (BBa_).
false
false
_234_
0
3299
9
Not in stock
false
-
false
Pascal Kraemer
annotation1983191
1
linkerHD04
range1983191
1
1
8
BBa_R0040
1
p(tetR)
TetR repressible promoter
2003-01-31T12:00:00Z
2015-05-08T01:14:14Z
Lutz, R., Bujard, H., <em>Nucleic Acids Research</em> (1997) 25, 1203-1210.
Released HQ 2013
Sequence for pTet inverting regulator driven by the TetR protein.</P>
false
true
_1_
0
24
7
In stock
false
<P> <P>BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.<P>
true
June Rhee, Connie Tao, Ty Thomson, Louis Waldman
annotation1986783
1
TetR 1
range1986783
1
1
19
annotation1986786
1
TetR 2
range1986786
1
26
44
annotation1986784
1
BBa_R0040
range1986784
1
1
54
annotation1986785
1
-35
range1986785
1
20
25
annotation1986787
1
-10
range1986787
1
43
48
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation1687
1
stop
range1687
1
34
34
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation7020
1
BBa_B0012
range7020
1
1
41
BBa_K150008
1
BamHI
BamHI restriction enzyme site
2008-10-20T11:00:00Z
2015-05-08T01:10:46Z
-
BamHI restriction site
false
true
_234_
0
3299
9
Not in stock
false
-
false
Pascal Kraemer
BBa_K150011
1
BBa_K150011
ColicinE9 Producer Controlled by 3OC6HSL Receiver Device
2008-10-20T11:00:00Z
2015-05-08T01:10:46Z
-
The constitutive expressed LuxR protein is able to build a complex with the AHL molecule. This complex function as transcription factor for the lux pR promoter and activates by this the colicinE9 production. This activation of the lux pR promoter can additionally lead to expression of the atypical lysis protein by read through of the terminator behind the colE9 gene and therefore to lysis of the cell and colcin release. The colicinE9 immunity protein located on the reverse strand is expressed under a constitutive promoter.
false
false
_234_
0
3299
9
It's complicated
false
-
false
Pascal Kraemer, Marika Ziesack, Kathrin Nussbaum and Andreas Kuehne
component2305672
1
BBa_K150010
component2305649
1
BBa_B0010
component2305648
1
BBa_K150100
component2305658
1
BBa_R0062
component2305641
1
BBa_K150107
component2305644
1
BBa_C0062
component2305666
1
BBa_K150008
component2305665
1
BBa_K150005
component2305656
1
BBa_K150100
component2305651
1
BBa_K150100
component2305652
1
BBa_B0012
component2305638
1
BBa_K150100
component2305663
1
BBa_K150104
component2305640
1
BBa_B0034
component2305633
1
BBa_R0040
annotation2305638
1
BBa_K150100
range2305638
1
55
62
annotation2305652
1
BBa_B0012
range2305652
1
958
998
annotation2305656
1
BBa_K150100
range2305656
1
999
1006
annotation2305663
1
BBa_K150104
range2305663
1
1062
1069
annotation2305640
1
BBa_B0034
range2305640
1
63
74
annotation2305666
1
BBa_K150008
range2305666
1
1083
1088
annotation2305633
1
BBa_R0040
range2305633
1
1
54
annotation2305644
1
BBa_C0062
range2305644
1
81
836
annotation2305648
1
BBa_K150100
range2305648
1
862
869
annotation2305658
1
BBa_R0062
range2305658
1
1007
1061
annotation2305672
1
BBa_K150010
range2305672
1
1089
3128
annotation2305651
1
BBa_K150100
range2305651
1
950
957
annotation2305649
1
BBa_B0010
range2305649
1
870
949
annotation2305665
1
BBa_K150005
range2305665
1
1070
1082
annotation2305641
1
BBa_K150107
range2305641
1
75
80
BBa_C0062
1
luxr
luxR repressor/activator, (no LVA?)
2003-01-31T12:00:00Z
2015-08-31T04:07:23Z
<em>V. fischeri</em> <genbank>AF170104</genbank>
Released HQ 2013
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. <p>The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.</p>
false
true
_1_
0
24
7
In stock
false
<P> <P>2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in <em>E. coli</em> with codons used at a similar frequency. <P>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
annotation7039
1
BBa_C0062
range7039
1
1
756
annotation1762
1
prefix
range1762
1
1
2
annotation2213986
1
Help:Barcodes
range2213986
1
757
781
annotation1765
1
A
range1765
1
492
492
annotation1764
1
T
range1764
1
174
174
annotation1766
1
luxR
range1766
1
1
750
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_K150100
1
BBa_K150100
linkerHD00
2008-10-20T11:00:00Z
2015-05-08T01:10:47Z
-
original scar from BioBricks
false
false
_234_
0
3314
9
Not in stock
false
-
false
Christian Moritz
BBa_K150107
1
BBa_K150107
linkerHD07
2008-10-20T11:00:00Z
2015-05-08T01:10:47Z
-
linkerHD07
false
false
_234_
0
3299
9
Not in stock
false
-
false
Pascal Kraemer
BBa_K150010
1
BBa_K150010
ColicinE9 operon
2008-10-20T11:00:00Z
2015-05-08T01:10:46Z
naturally occurring on the pColE9-J plasmid
This colicinE1 operon contains all three important genes for colicinE9 expression and release. The colE9 gene codes for the lethal colicinE9 protein. The gene for the colE9 immunity protein is responsible for the immunity of the producing host bacteria to the colicinE9 and is therefore controlled by a constitutive promoter. Expression of the atypical lysis protein leads finally to the lysis of the host bacteria and thereby to the release of the colicinE9.
false
false
_234_
0
3299
9
Not in stock
false
-
false
Pascal Kraemer
annotation1983616
1
colE9 immunity protein
range1983616
1
622
882
annotation1983617
1
atypical lysis protein
range1983617
1
944
1024
annotation1983615
1
colE9 protein
range1983615
1
1
620
annotation1983618
1
colE5 immunity protein
range1983618
1
1423
1674
annotation1983619
1
canonical lysis protein
range1983619
1
1772
1915
BBa_R0062_sequence
1
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaa
BBa_B0034_sequence
1
aaagaggagaaa
BBa_K150107_sequence
1
tactag
BBa_K150011_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaggaaacagaaccggatccatcgatttgcccatgacccaatggctggcggtcacagaatgtggcaaatggccgggcttaaagctcagcgggcgcagacggatgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgcattaagtgccgcgcaggagcgccgcaaacagaaggaaaataaagaaaaggacgctaaggataaattagataaggagagtaaacggaataagccagggaaggcgacaggtaaaggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctgataagttgcgtgataaagaatttaaaagcttcgacgattttcggaaggctgtatgggaagaggtgtcgaaagatcctgagcttagtaaaaatttaaacccaagcaataagtctagtgtttcaaaaggttattctctgtttactccaaagaatcaacaggtcggagggagaaaagtctatgaacttcatcatgacaagccaattagtcaaggtggtgaggtttatgacatggataatatccgagtgactacacctaagcgacatatcgatattcaccgaggtaagtaaaatggaactgaagcatagcattagtgattatacagaagctgaatttttacagcttgtaacaacaatttgtaatgcggacacttccagtgaagaagaactggttaaattggttacacactttgaggaaatgactgagcaccctagtggtagtgatttaatatattacccaaaagaaggtgatgatgactcaccttcaggtattgtaaacacagtaaaacaatggcgagccgctaacggtaagtcaggatttaaacagggctaaaatatgagtgccggttgtttaaggatgaatggctggcattctttcacaacaaggagtcgttatgaaaaaaataacagggattattttattgcttcttgcagtcattattctgtctgcatggggttctaagccgaaaacctagaaaattccgtaaccaaagccagtaattgacagatttgcatgacgttgaataggtgacgggttatgtgacgaaatctgatgcagaaatcgttgtttcagtgacagtcactcaatcggtcgtttatgtgacaacccacgccgttactggtcgcggaaaaatccaggtttccggcctggaaccccgaaatgatccagcaacagtgtatggttctcctggtaaatatgttgttgtcaatgatcgtactggtgaggttactcagattagtgataagacagatccgggttgggtggacgattcgagagttcaatggggaaataaaaatggggtggacgattcgagagttcaatggggaaataaaaatgaccaataaattatttgaacatacggtgttatatgatagtggtgatgccttttttgaattaaaaggaaatgcttctatgaagttatcaccaaaagctgcaatagaagtttgtaatgaagcagcgaaaaaaggcttatggattttgggcattgatggtgggcattggctgaatcctggattcaggatagatagttcagcatcatggacatatgatatgccggaggaatacaaatcaaaaacccctgaaaataatagattggctattgaaaatattaaagatgatattgagaatggatacactgctttcattatcacgttaaagatgtaaatagtgttatagaattttatgtttcatggatgatttcaacctttggatttcaggtttttatggatgaatgcctgagtccatatatacaaggaacagtatgaaaaaaataacagggattattttattgcttcttgcagccattattcttgctgcatgtcaggcaaactatatccgcgatgttcagggcgggactgtatccccgtcatcctcagctgaactgaccggattagcaacgcagtaacccgaaatcctctttgacaaaaacaaagcgtgtcaggctgattctgatgcgctttttttttgaaatgtcacaaaagttctcatgtg
BBa_K150005_sequence
1
aggaaacagaacc
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_K150104_sequence
1
tactagag
BBa_K150100_sequence
1
tactagag
BBa_R0040_sequence
1
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
BBa_K150008_sequence
1
ggatcc
BBa_K150010_sequence
1
atcgatttgcccatgacccaatggctggcggtcacagaatgtggcaaatggccgggcttaaagctcagcgggcgcagacggatgtaaataataagcaggctgcatttgatgctgctgcaaaagagaagtcagatgctgatgctgcattaagtgccgcgcaggagcgccgcaaacagaaggaaaataaagaaaaggacgctaaggataaattagataaggagagtaaacggaataagccagggaaggcgacaggtaaaggtaaaccagttggtgataaatggctggatgatgcaggtaaagattcaggagcgccaattccagatcgcattgctgataagttgcgtgataaagaatttaaaagcttcgacgattttcggaaggctgtatgggaagaggtgtcgaaagatcctgagcttagtaaaaatttaaacccaagcaataagtctagtgtttcaaaaggttattctctgtttactccaaagaatcaacaggtcggagggagaaaagtctatgaacttcatcatgacaagccaattagtcaaggtggtgaggtttatgacatggataatatccgagtgactacacctaagcgacatatcgatattcaccgaggtaagtaaaatggaactgaagcatagcattagtgattatacagaagctgaatttttacagcttgtaacaacaatttgtaatgcggacacttccagtgaagaagaactggttaaattggttacacactttgaggaaatgactgagcaccctagtggtagtgatttaatatattacccaaaagaaggtgatgatgactcaccttcaggtattgtaaacacagtaaaacaatggcgagccgctaacggtaagtcaggatttaaacagggctaaaatatgagtgccggttgtttaaggatgaatggctggcattctttcacaacaaggagtcgttatgaaaaaaataacagggattattttattgcttcttgcagtcattattctgtctgcatggggttctaagccgaaaacctagaaaattccgtaaccaaagccagtaattgacagatttgcatgacgttgaataggtgacgggttatgtgacgaaatctgatgcagaaatcgttgtttcagtgacagtcactcaatcggtcgtttatgtgacaacccacgccgttactggtcgcggaaaaatccaggtttccggcctggaaccccgaaatgatccagcaacagtgtatggttctcctggtaaatatgttgttgtcaatgatcgtactggtgaggttactcagattagtgataagacagatccgggttgggtggacgattcgagagttcaatggggaaataaaaatggggtggacgattcgagagttcaatggggaaataaaaatgaccaataaattatttgaacatacggtgttatatgatagtggtgatgccttttttgaattaaaaggaaatgcttctatgaagttatcaccaaaagctgcaatagaagtttgtaatgaagcagcgaaaaaaggcttatggattttgggcattgatggtgggcattggctgaatcctggattcaggatagatagttcagcatcatggacatatgatatgccggaggaatacaaatcaaaaacccctgaaaataatagattggctattgaaaatattaaagatgatattgagaatggatacactgctttcattatcacgttaaagatgtaaatagtgttatagaattttatgtttcatggatgatttcaacctttggatttcaggtttttatggatgaatgcctgagtccatatatacaaggaacagtatgaaaaaaataacagggattattttattgcttcttgcagccattattcttgctgcatgtcaggcaaactatatccgcgatgttcagggcgggactgtatccccgtcatcctcagctgaactgaccggattagcaacgcagtaacccgaaatcctctttgacaaaaacaaagcgtgtcaggctgattctgatgcgctttttttttgaaatgtcacaaaagttctcatgtg
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_C0062_sequence
1
atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z