LOCUS       BBa_K150013             2112 bp    DNA     linear       12-May-2024
DEFINITION  ColicinE9 Producer Controlled by 3OC6HSL Receiver Device (short)
ACCESSION   BBa_K150013
VERSION     BBa_K150013.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    55..62
                     /label=BBa_K150100
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    75..80
                     /label=BBa_K150107
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     misc_feature    862..869
                     /label=BBa_K150100
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     misc_feature    950..957
                     /label=BBa_K150100
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     misc_feature    999..1006
                     /label=BBa_K150100
     misc_feature    1007..1026
                     /label=LuxR/HSL
     promoter        1026..1031
                     /label=-35
     promoter        1048..1053
                     /label=-10
     misc_feature    1059..1059
                     /label=start
     misc_feature    1007..1061
                     /label=BBa_R0062
     promoter        1007..1061
                     /label=BBa_R0062
     misc_feature    1062..1069
                     /label=linkerHD04
     misc_feature    1062..1069
                     /label=BBa_K150104
     RBS             1070..1082
                     /label=BBa_K150005
     RBS             1070..1082
                     /label=BBa_K150005
     misc_feature    1083..1088
                     /label=BBa_K150008
     CDS             1089..1708
                     /label=colE9 protein
     CDS             1710..1970
                     /label=colE9 immunity protein
     CDS             2032..2112
                     /label=atypical lysis protein
     misc_feature    1089..2112
                     /label=BBa_K150012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagacct gtaggatcgt
     1021 acaggtttac gcaagaaaat ggtttgttat agtcgaataa atactagaga ggaaacagaa
     1081 ccggatccat cgatttgccc atgacccaat ggctggcggt cacagaatgt ggcaaatggc
     1141 cgggcttaaa gctcagcggg cgcagacgga tgtaaataat aagcaggctg catttgatgc
     1201 tgctgcaaaa gagaagtcag atgctgatgc tgcattaagt gccgcgcagg agcgccgcaa
     1261 acagaaggaa aataaagaaa aggacgctaa ggataaatta gataaggaga gtaaacggaa
     1321 taagccaggg aaggcgacag gtaaaggtaa accagttggt gataaatggc tggatgatgc
     1381 aggtaaagat tcaggagcgc caattccaga tcgcattgct gataagttgc gtgataaaga
     1441 atttaaaagc ttcgacgatt ttcggaaggc tgtatgggaa gaggtgtcga aagatcctga
     1501 gcttagtaaa aatttaaacc caagcaataa gtctagtgtt tcaaaaggtt attctccgtt
     1561 tactccaaag aatcaacagg tcggagggag aaaagtctat gaacttcatc atgacaagcc
     1621 aattagtcaa ggtggtgagg tttatgacat ggataatatc cgagtgacta cacctaagcg
     1681 acatatcgat attcaccgag gtaagtaaaa tggaactgaa gcatagcatt agtgattata
     1741 cagaagctga atttttacag cttgtaacaa caatttgtaa tgcggacact tccagtgaag
     1801 aagaactggt taaattggtt acacactttg aggaaatgac tgagcaccct agtggtagtg
     1861 atttaatata ttacccaaaa gaaggtgatg atgactcacc ttcaggtatt gtaaacacag
     1921 taaaacaatg gcgagccgct aacggtaagt caggatttaa acagggctaa aatatgagtg
     1981 ccggttgttt aaggatgaat ggctggcatt ctttcacaac aaggagtcgt tatgaaaaaa
     2041 ataacaggga ttattttatt gcttcttgca gtcattattc tgtctgcatg gggttctaag
     2101 ccgaaaacct ag
//