BBa_B0010
1
BBa_B0010
T1 from E. coli rrnB
2003-11-19T12:00:00Z
2015-08-31T04:07:20Z
Transcriptional terminator consisting of a 64 bp stem-loop.
false
false
_1_
0
24
7
In stock
false
true
Randy Rettberg
annotation7018
1
BBa_B0010
range7018
1
1
80
annotation4184
1
stem_loop
range4184
1
12
55
BBa_B0015
1
BBa_B0015
double terminator (B0010-B0012)
2003-07-16T11:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
Double terminator consisting of BBa_B0010 and BBa_B0012
false
true
_1_
0
24
7
In stock
false
true
Reshma Shetty
component1916612
1
BBa_B0012
component1916610
1
BBa_B0010
annotation1916610
1
BBa_B0010
range1916610
1
1
80
annotation1916612
1
BBa_B0012
range1916612
1
89
129
BBa_B0034
1
BBa_B0034
RBS (Elowitz 1999) -- defines RBS efficiency
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Released HQ 2013
RBS based on Elowitz repressilator.
false
true
_1_
0
24
7
In stock
false
Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS.
Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a>
true
Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
annotation23325
1
conserved
range23325
1
5
8
BBa_J23119
1
BBa_J23119
constitutive promoter family member
2006-08-23T11:00:00Z
2015-08-31T04:08:40Z
Overlap extension of synthetic oligonucleotides
Released HQ 2013
Later
false
true
_52_
0
483
95
In stock
false
N/A
true
John Anderson
BBa_K1507006
1
BBa_K1507006
Rose fragrance generator (J23119-RBS34-CCD1-B0015)
2014-10-06T11:00:00Z
2015-05-08T01:10:47Z
CCD1 is cloned from Arabidopsis thaliana
This part can convert beta carotene into beta ionone (generally known as rose fragrance when condensation is low)
false
false
_1887_
0
23158
9
Not in stock
false
You have to use a special plasmid which can generate beta carotene.
false
Willy Hsu
component2398734
1
BBa_J23119
component2398736
1
BBa_B0034
component2398744
1
BBa_B0015
component2398737
1
BBa_K1507002
annotation2398736
1
BBa_B0034
range2398736
1
44
55
annotation2398744
1
BBa_B0015
range2398744
1
1687
1815
annotation2398734
1
BBa_J23119
range2398734
1
1
35
annotation2398737
1
BBa_K1507002
range2398737
1
62
1678
BBa_B0012
1
BBa_B0012
TE from coliphageT7
2003-01-31T12:00:00Z
2015-08-31T04:07:20Z
Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
Released HQ 2013
Transcription terminator for the <i>E.coli</i> RNA polymerase.
false
false
_1_
0
24
7
In stock
false
<P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
false
Reshma Shetty
annotation7020
1
BBa_B0012
range7020
1
1
41
annotation1690
1
polya
range1690
1
28
41
annotation1686
1
T7 TE
range1686
1
8
27
annotation1687
1
stop
range1687
1
34
34
BBa_K1507002
1
BBa_K1507002
Carotenoid Cleavage Dioxygenase 1 (CCD1)
2014-10-06T11:00:00Z
2015-05-08T01:10:47Z
cloned from Arabidopsis thaliana.
Encodes a protein with 9-cis-epoxycarotenoid dioxygenase activity. The enzyme was shown to act on a variety of carotenoid including β-carotene, lutein, zeaxanthin, and all-trans-violaxanthin. When those compounds are used as substrates, the major reaction product detected is a C14 dialdehyde: 4,9-dimethyldodeca-2,4,6,8,10-pentaene-1,12-dial. The enzyme did not cleave as efficiently carotenoids containing 9-cis-double or allenic bonds.
false
false
_1887_
0
23158
9
It's complicated
false
You have to find a competent cell which is suitable to express eukaryotic genes
false
Willy Hsu
BBa_K1507002_sequence
1
atggcggagaaactcagtgatggcagcagcatcatctcagtccatcctagaccctccaagggtttctcctcgaagcttctcgatcttctcgagagacttgttgtcaagctcatgcacgatgcttctctccctctccactacctctcaggcaacttcgctcccatccgtgatgaaactcctcccgtcaaggatctccccgtccatggatttcttcccgaatgcttgaatggtgaatttgtgagggttggtccaaaccccaagtttgatgctgtcgctggatatcactggtttgatggagatgggatgattcatggggtacgcatcaaagatgggaaagctacttatgtttctcgatatgttaagacatcacgtcttaagcaggaagagttcttcggagctgccaaattcatgaagattggtgaccttaaggggtttttcggattgctaatggtcaatgtccaacagctgagaacgaagctcaaaatattggacaacacttatggaaatggaactgccaatacagcactcgtatatcaccatggaaaacttctagcattacaggaggcagataagccgtacgtcatcaaagttttggaagatggagacctgcaaactcttggtataatagattatgacaagagattgacccactccttcactgctcacccaaaagttgacccggttacgggtgaaatgtttacattcggctattcgcatacgccaccttatctcacatacagagttatctcgaaagatggcattatgcatgacccagtcccaattactatatcagagcctatcatgatgcatgattttgctattactgagacttatgcaatcttcatggatcttcctatgcacttcaggccaaaggaaatggtgaaagagaagaaaatgatatactcatttgatcccacaaaaaaggctcgttttggtgttcttccacgctatgccaaggatgaacttatgattagatggtttgagcttcccaactgctttattttccacaacgccaatgcttgggaagaagaggatgaagtcgtcctcatcacttgtcgtcttgagaatccagatcttgacatggtcagtgggaaagtgaaagaaaaactcgaaaattttggcaacgaactgtacgaaatgagattcaacatgaaaacgggctcagcttctcaaaaaaaactatccgcatctgcggttgatttccccagaatcaatgagtgctacaccggaaagaaacagagatacgtatatggaacaattctggacagtatcgcaaaggttaccggaatcatcaagtttgatctgcatgcagaagctgagacagggaaaagaatgctggaagtaggaggtaatatcaaaggaatatatgacctgggagaaggcagatatggttcagaggctatctatgttccgcgtgagacagcagaagaagacgacggttacttgatattctttgttcatgatgaaaacacagggaaatcatgcgtgactgtgatagacgcaaaaacaatgtcggctgaaccggtggcagtggtggagctgccgcacagggtcccatatggcttccatgccttgtttgttacagaggaacaactccaggaacaaactcttatataa
BBa_B0010_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
BBa_B0034_sequence
1
aaagaggagaaa
BBa_B0012_sequence
1
tcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_K1507006_sequence
1
ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatggcggagaaactcagtgatggcagcagcatcatctcagtccatcctagaccctccaagggtttctcctcgaagcttctcgatcttctcgagagacttgttgtcaagctcatgcacgatgcttctctccctctccactacctctcaggcaacttcgctcccatccgtgatgaaactcctcccgtcaaggatctccccgtccatggatttcttcccgaatgcttgaatggtgaatttgtgagggttggtccaaaccccaagtttgatgctgtcgctggatatcactggtttgatggagatgggatgattcatggggtacgcatcaaagatgggaaagctacttatgtttctcgatatgttaagacatcacgtcttaagcaggaagagttcttcggagctgccaaattcatgaagattggtgaccttaaggggtttttcggattgctaatggtcaatgtccaacagctgagaacgaagctcaaaatattggacaacacttatggaaatggaactgccaatacagcactcgtatatcaccatggaaaacttctagcattacaggaggcagataagccgtacgtcatcaaagttttggaagatggagacctgcaaactcttggtataatagattatgacaagagattgacccactccttcactgctcacccaaaagttgacccggttacgggtgaaatgtttacattcggctattcgcatacgccaccttatctcacatacagagttatctcgaaagatggcattatgcatgacccagtcccaattactatatcagagcctatcatgatgcatgattttgctattactgagacttatgcaatcttcatggatcttcctatgcacttcaggccaaaggaaatggtgaaagagaagaaaatgatatactcatttgatcccacaaaaaaggctcgttttggtgttcttccacgctatgccaaggatgaacttatgattagatggtttgagcttcccaactgctttattttccacaacgccaatgcttgggaagaagaggatgaagtcgtcctcatcacttgtcgtcttgagaatccagatcttgacatggtcagtgggaaagtgaaagaaaaactcgaaaattttggcaacgaactgtacgaaatgagattcaacatgaaaacgggctcagcttctcaaaaaaaactatccgcatctgcggttgatttccccagaatcaatgagtgctacaccggaaagaaacagagatacgtatatggaacaattctggacagtatcgcaaaggttaccggaatcatcaagtttgatctgcatgcagaagctgagacagggaaaagaatgctggaagtaggaggtaatatcaaaggaatatatgacctgggagaaggcagatatggttcagaggctatctatgttccgcgtgagacagcagaagaagacgacggttacttgatattctttgttcatgatgaaaacacagggaaatcatgcgtgactgtgatagacgcaaaaacaatgtcggctgaaccggtggcagtggtggagctgccgcacagggtcccatatggcttccatgccttgtttgttacagaggaacaactccaggaacaaactcttatataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
BBa_J23119_sequence
1
ttgacagctagctcagtcctaggtataatgctagc
BBa_B0015_sequence
1
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z