BBa_K1510930

BBa_K1510930 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1510930

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Wang,Ching-Yun

Date created: 2014-10-09 11:00:00

Date modified: 2015-05-08 01:10:48

Constitutive promoter and RBS region for Streptococcus mutans transcription.

Details

• Types: DnaRegion
• Roles: Regulatory; promoter
• Sequences: BBa_K1510930_sequence (Version 1)

Description

We are going to express the luxI gene which would code for the AHL quorum sensing signal in Streptococcus mutans. The goal is to let the engineered probiotics with luxR protein bind with AHL and yield the downstream expression of S.mutans killing module.

Notes

The promoter and RBS region would only be in need in the functional measurement stage of our project so that we could test whether the S.mutans with luxI gene below our constitutive promoter and RBS region could normally express the quorum sensing signal AHL or not.
Whereas, in the working circuit, this promoter and RBS region won't be added because the luxI gene and its terminator are ligated to a complete sequence with CSP-sensing promoter region.

Source

The constitutive promoter and RBS region is obtained from the genome of Streptococcus mutans by self-designed PCr primers.

Other Properties

• igem#sampleStatus: In stock
• igem#status: Available
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1510930/1

Member of these Collections

• iGEM Parts Registry