LOCUS BBa_K1520514 4197 bp DNA linear 23-May-2024 DEFINITION MC31-rbs-tetR-Ter-PtetO-rbs-rfp-Ter-Plac-rbs-tetR-Ter-Pcons2-rbs-la cI-Ter ACCESSION BBa_K1520514 VERSION BBa_K1520514.1 FEATURES Location/Qualifiers promoter 1..132 /label=BBa_K1520002 RBS 141..158 /label=rbs CDS 159..843 /label=tetR misc_feature 141..843 /label=BBa_K1520505 misc_feature 852..870 /label=TetR 1 misc_feature 852..905 /label=BBa_R0040 promoter 871..876 /label=-35 misc_feature 877..895 /label=TetR 2 promoter 894..899 /label=-10 promoter 852..905 /label=BBa_R0040 misc_feature 914..1645 /label=BBa_K1520500 terminator 1654..1782 /label=BBa_K1520029 misc_feature 852..1782 /label=BBa_K1520014 CDS 1791..1878 /label=end of LacI coding region (inactive) misc_feature 1791..1990 /label=BBa_R0010 misc_feature 1879..1916 /label=CAP binding site promoter 1927..1932 /label=-35 promoter 1951..1956 /label=-10 misc_feature 1956..1990 /label=LacI binding site misc_feature 1963..1963 /label=start promoter 1791..1990 /label=BBa_R0010 RBS 1999..2016 /label=rbs CDS 2017..2701 /label=tetR misc_feature 1999..2701 /label=BBa_K1520505 terminator 2710..2838 /label=BBa_K1520029 misc_feature 1791..2838 /label=BBa_K1520015 misc_feature 852..2838 /label=BBa_K1520025 promoter 2847..2881 /label=BBa_J23100 RBS 2890..2907 /label=rbs CDS 2908..4060 /label=lacI misc_feature 2890..4060 /label=BBa_K1520502 terminator 4069..4197 /label=BBa_K1520029 misc_feature 2847..4197 /label=BBa_K1520008 misc_feature 852..4197 /label=BBa_K1520026 ORIGIN 1 aaacaaacaa agctgtcacc ggatgtgctt tccggtctga tgagtccgtg tccgggagtg 61 cgtgcacctg cacctggggg ggtgttcgct tgggacggga cgaggacgaa acagcaaaaa 121 gaaaaataaa aatactagag aaagaggaga aattaagcat gtccagatta gataaaagta 181 aagtgattaa cagcgcatta gagctgctta atgaggtcgg aatcgaaggt ttaacaaccc 241 gtaaactcgc ccagaagcta ggtgtagagc agcctacatt gtattggcat gtaaaaaata 301 agcgggcttt gctcgacgcc ttagccattg agatgttaga taggcaccat actcactttt 361 gccctttaga aggggaaagc tggcaagatt ttttacgtaa taacgctaaa agttttagat 421 gtgctttact aagtcatcgc gatggagcaa aagtacattt aggtacacgg cctacagaaa 481 aacagtatga aactctcgaa aatcaattag cctttttatg ccaacaaggt ttttcactag 541 agaatgcatt atatgcactc agcgctgtgg ggcattttac tttaggttgc gtattggaag 601 atcaagagca tcaagtcgct aaagaagaaa gggaaacacc tactactgat agtatgccgc 661 cattattacg acaagctatc gaattatttg atcaccaagg tgcagagcca gccttcttat 721 tcggccttga attgatcata tgcggattag aaaaacaact taaatgtgaa agtgggtccg 781 ctgcaaacga cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat 841 cactactaga gtccctatca gtgatagaga ttgacatccc tatcagtgat agagatactg 901 agcactacta gagaaagagg agaaattaag catggtgagc aagggcgagg aggataacat 961 ggccatcatc aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca 1021 cgagttcgag atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa 1081 gctgaaggtg accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt 1141 catgtacggc tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct 1201 gtccttcccc gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt 1261 gaccgtgacc caggactcct ccttgcagga cggcgagttc atctacaagg tgaagctgcg 1321 cggcaccaac ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc 1381 ctcctccgag cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct 1441 gaagctgaag gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa 1501 gcccgtgcag ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa 1561 cgaggactac accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg 1621 catggacgag ctgtacaagt aataatacta gagccaggca tcaaataaaa cgaaaggctc 1681 agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct ctctactaga 1741 gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag caatacgcaa 1801 accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca ggtttcccga 1861 ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc attaggcacc 1921 ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga gcggataaca 1981 atttcacaca tactagagaa agaggagaaa ttaagcatgt ccagattaga taaaagtaaa 2041 gtgattaaca gcgcattaga gctgcttaat gaggtcggaa tcgaaggttt aacaacccgt 2101 aaactcgccc agaagctagg tgtagagcag cctacattgt attggcatgt aaaaaataag 2161 cgggctttgc tcgacgcctt agccattgag atgttagata ggcaccatac tcacttttgc 2221 cctttagaag gggaaagctg gcaagatttt ttacgtaata acgctaaaag ttttagatgt 2281 gctttactaa gtcatcgcga tggagcaaaa gtacatttag gtacacggcc tacagaaaaa 2341 cagtatgaaa ctctcgaaaa tcaattagcc tttttatgcc aacaaggttt ttcactagag 2401 aatgcattat atgcactcag cgctgtgggg cattttactt taggttgcgt attggaagat 2461 caagagcatc aagtcgctaa agaagaaagg gaaacaccta ctactgatag tatgccgcca 2521 ttattacgac aagctatcga attatttgat caccaaggtg cagagccagc cttcttattc 2581 ggccttgaat tgatcatatg cggattagaa aaacaactta aatgtgaaag tgggtccgct 2641 gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta gtgtagatca 2701 ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt 2761 ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg 2821 ggcctttctg cgtttatata ctagagttga cggctagctc agtcctaggt acagtgctag 2881 ctactagaga aagaggagaa attaagcatg gtgaatgtga aaccagtaac gttatacgat 2941 gtcgcagagt atgccggtgt ctcttatcag accgtttccc gcgtggtgaa ccaggccagc 3001 cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga tggcggagct gaattacatt 3061 cccaaccgcg tggcacaaca actggcgggc aaacagtcgt tgctgattgg cgttgccacc 3121 tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat 3181 caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa 3241 gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg 3301 gatgaccagg atgccattgc tgtggaagct gcctgcacta atgttccggc gttatttctt 3361 gatgtctctg accagacacc catcaacagt attattttct cccatgaaga cggtacgcga 3421 ctgggcgtgg agcatctggt cgcattgggt caccagcaaa tcgcgctgtt agcgggccca 3481 ttaagttctg tctcggcgcg tctgcgtctg gctggctggc ataaatatct cactcgcaat 3541 caaattcagc cgatagcgga acgggaaggc gactggagtg ccatgtccgg ttttcaacaa 3601 accatgcaaa tgctgaatga gggcatcgtt cccactgcga tgctggttgc caacgatcag 3661 atggcgctgg gcgcaatgcg cgccattacc gagtccgggc tgcgcgttgg tgcggatatc 3721 tcggtagtgg gatacgacga taccgaagac agctcatgtt atatcccgcc gttaaccacc 3781 atcaaacagg attttcgcct gctggggcaa accagcgtgg accgcttgct gcaactctct 3841 cagggccagg cggtgaaggg caatcagctg ttgcccgtct cactggtgaa aagaaaaacc 3901 accctggcgc ccaatacgca aaccgcctct ccccgcgcgt tggccgattc attaatgcag 3961 ctggcacgac aggtttcccg actggaaagc gggcaggctg caaacgacga aaactacgct 4021 ttagtagctt aataactctg atagtgctag tgtagatctc tactagagcc aggcatcaaa 4081 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga 4141 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata //