LOCUS       BBa_K1520520            3596 bp    DNA     linear       18-May-2024
DEFINITION  PgolTS-golS-PgolB-tetR-Ter-PtetO-rfp-Ter-Plac-tetR-Ter
ACCESSION   BBa_K1520520
VERSION     BBa_K1520520.1  
FEATURES             Location/Qualifiers
     promoter        1..200
                     /label=PgolTS
     CDS             212..553
                     /label=golS
     promoter        554..753
                     /label=PgolB
     promoter        1..753
                     /label=BBa_K1520506
     RBS             762..779
                     /label=rbs
     CDS             780..1464
                     /label=tetR
     misc_feature    762..1464
                     /label=BBa_K1520505
     terminator      1473..1601
                     /label=BBa_K1520029
     misc_feature    1..1601
                     /label=BBa_K1520518
     misc_feature    1610..1628
                     /label=TetR 1
     misc_feature    1610..1663
                     /label=BBa_R0040
     promoter        1629..1634
                     /label=-35
     misc_feature    1635..1653
                     /label=TetR 2
     promoter        1652..1657
                     /label=-10
     promoter        1610..1663
                     /label=BBa_R0040
     misc_feature    1672..2403
                     /label=BBa_K1520500
     terminator      2412..2540
                     /label=BBa_K1520029
     misc_feature    1610..2540
                     /label=BBa_K1520014
     CDS             2549..2636
                     /label=end of LacI coding region (inactive)
     misc_feature    2549..2748
                     /label=BBa_R0010
     misc_feature    2637..2674
                     /label=CAP binding site
     promoter        2685..2690
                     /label=-35
     promoter        2709..2714
                     /label=-10
     misc_feature    2714..2748
                     /label=LacI binding site
     misc_feature    2721..2721
                     /label=start
     promoter        2549..2748
                     /label=BBa_R0010
     RBS             2757..2774
                     /label=rbs
     CDS             2775..3459
                     /label=tetR
     misc_feature    2757..3459
                     /label=BBa_K1520505
     terminator      3468..3596
                     /label=BBa_K1520029
     misc_feature    2549..3596
                     /label=BBa_K1520015
ORIGIN
        1 acaacgaagg aaaggtcaag cgttcctgac gggtttttac ggggcgtggg tcggcatcgt
       61 ggcgtaaatg tctcgcatca tcctctttta tgagccatct cacattctcg ccgaaccgtg
      121 cagcctgaat acgcttgacc ttcccacaat ggcaagcttt aggctttctg ataccgaata
      181 gtcaggatgg ggaagtcgtc ggaggagcgt catgaacatc ggtaaagcag ctaaagcatc
      241 gaaagtctcg gccaaaatga ttcgctacta tgaacagatt ggtctgattc ccgcggcaag
      301 tcggacggat tccggctatc gggcctatac ccaggctgat gttaatcaat tgcattttat
      361 acgccgcgcg cgcgacctcg gtttttcagt tgctgaaatc agcgacttac tgaatctttg
      421 gaataaccag tcgcggcaaa gcgctgacgt caaacgcctg gcgcagacgc acattgatga
      481 actggacaga cgtatccaga acatgcagca catggcgcaa accctcaaag cgctgattca
      541 ctgctgcgcc ggcgacgcgc tgccagattg ccccattctg catacgcttg gacaacctga
      601 cgatagcgag ccggaggcgc gtaccggagc ggtattgcga cgtcctcgtc gccacggact
      661 ggcaaagcgt ctgtaagtcc tgagattacg cttgaccttc caacactggc aaggtccaga
      721 ctggcaacag ttcccacaca aaaggagttc acttactaga gaaagaggag aaattaagca
      781 tgtccagatt agataaaagt aaagtgatta acagcgcatt agagctgctt aatgaggtcg
      841 gaatcgaagg tttaacaacc cgtaaactcg cccagaagct aggtgtagag cagcctacat
      901 tgtattggca tgtaaaaaat aagcgggctt tgctcgacgc cttagccatt gagatgttag
      961 ataggcacca tactcacttt tgccctttag aaggggaaag ctggcaagat tttttacgta
     1021 ataacgctaa aagttttaga tgtgctttac taagtcatcg cgatggagca aaagtacatt
     1081 taggtacacg gcctacagaa aaacagtatg aaactctcga aaatcaatta gcctttttat
     1141 gccaacaagg tttttcacta gagaatgcat tatatgcact cagcgctgtg gggcatttta
     1201 ctttaggttg cgtattggaa gatcaagagc atcaagtcgc taaagaagaa agggaaacac
     1261 ctactactga tagtatgccg ccattattac gacaagctat cgaattattt gatcaccaag
     1321 gtgcagagcc agccttctta ttcggccttg aattgatcat atgcggatta gaaaaacaac
     1381 ttaaatgtga aagtgggtcc gctgcaaacg acgaaaacta cgctttagta gcttaataac
     1441 actgatagtg ctagtgtaga tcactactag agccaggcat caaataaaac gaaaggctca
     1501 gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg gtgaacgctc tctactagag
     1561 tcacactggc tcaccttcgg gtgggccttt ctgcgtttat atactagagt ccctatcagt
     1621 gatagagatt gacatcccta tcagtgatag agatactgag cactactaga gaaagaggag
     1681 aaattaagca tggtgagcaa gggcgaggag gataacatgg ccatcatcaa ggagttcatg
     1741 cgcttcaagg tgcacatgga gggctccgtg aacggccacg agttcgagat cgagggcgag
     1801 ggcgagggcc gcccctacga gggcacccag accgccaagc tgaaggtgac caagggtggc
     1861 cccctgccct tcgcctggga catcctgtcc cctcagttca tgtacggctc caaggcctac
     1921 gtgaagcacc ccgccgacat ccccgactac ttgaagctgt ccttccccga gggcttcaag
     1981 tgggagcgcg tgatgaactt cgaggacggc ggcgtggtga ccgtgaccca ggactcctcc
     2041 ttgcaggacg gcgagttcat ctacaaggtg aagctgcgcg gcaccaactt cccctccgac
     2101 ggccccgtaa tgcagaagaa gaccatgggc tgggaggcct cctccgagcg gatgtacccc
     2161 gaggacggcg ccctgaaggg cgagatcaag cagaggctga agctgaagga cggcggccac
     2221 tacgacgctg aggtcaagac cacctacaag gccaagaagc ccgtgcagct gcccggcgcc
     2281 tacaacgtca acatcaagtt ggacatcacc tcccacaacg aggactacac catcgtggaa
     2341 cagtacgaac gcgccgaggg ccgccactcc accggcggca tggacgagct gtacaagtaa
     2401 taatactaga gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg
     2461 ttttatctgt tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg
     2521 tgggcctttc tgcgtttata tactagagca atacgcaaac cgcctctccc cgcgcgttgg
     2581 ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc
     2641 aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt
     2701 ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagaaag
     2761 aggagaaatt aagcatgtcc agattagata aaagtaaagt gattaacagc gcattagagc
     2821 tgcttaatga ggtcggaatc gaaggtttaa caacccgtaa actcgcccag aagctaggtg
     2881 tagagcagcc tacattgtat tggcatgtaa aaaataagcg ggctttgctc gacgccttag
     2941 ccattgagat gttagatagg caccatactc acttttgccc tttagaaggg gaaagctggc
     3001 aagatttttt acgtaataac gctaaaagtt ttagatgtgc tttactaagt catcgcgatg
     3061 gagcaaaagt acatttaggt acacggccta cagaaaaaca gtatgaaact ctcgaaaatc
     3121 aattagcctt tttatgccaa caaggttttt cactagagaa tgcattatat gcactcagcg
     3181 ctgtggggca ttttacttta ggttgcgtat tggaagatca agagcatcaa gtcgctaaag
     3241 aagaaaggga aacacctact actgatagta tgccgccatt attacgacaa gctatcgaat
     3301 tatttgatca ccaaggtgca gagccagcct tcttattcgg ccttgaattg atcatatgcg
     3361 gattagaaaa acaacttaaa tgtgaaagtg ggtccgctgc aaacgacgaa aactacgctt
     3421 tagtagctta ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat
     3481 aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa
     3541 cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//