LOCUS BBa_K1521002 4719 bp DNA linear 20-May-2024 DEFINITION LasR functional construction + GFP reporter ACCESSION BBa_K1521002 VERSION BBa_K1521002.1 FEATURES Location/Qualifiers promoter 1..237 /label=promoter promoter 1..237 /label=BBa_K823003 RBS 246..257 /label=rbs CDS 264..983 /label=cds misc_feature 246..983 /label=BBa_K1521001 stem_loop 1003..1046 /label=stem_loop misc_feature 992..1071 /label=BBa_B0010 terminator 992..1071 /label=BBa_B0010 stem_loop 1087..1106 /label=T7 TE misc_feature 1113..1113 /label=stop polyA_site 1107..1120 /label=polya misc_feature 1080..1120 /label=BBa_B0012 terminator 1080..1120 /label=BBa_B0012 terminator 992..1120 /label=BBa_B0015 promoter 1129..1365 /label=promoter promoter 1129..1365 /label=BBa_K823003 RBS 1374..1388 /label=RBS-1\Strong RBS 1381..1385 /label=RBS misc_feature 1374..1388 /label=BBa_B0030 RBS 1374..1388 /label=BBa_B0030 CDS 1395..1997 /label=LasI misc_feature 1998..2030 /label=LVA misc_feature 2037..2061 /label=Help:Barcodes CDS 1395..2061 /label=BBa_C0078 misc_feature 2070..2108 /label=B1006 stem_loop 2079..2100 /label=modified thr terminator polyA_site 2101..2108 /label=PolyA polyA_site 2070..2078 /label=PolyA terminator 2070..2108 /label=BBa_B1006 misc_feature 2162..2179 /label=OP2 misc_feature 2222..2241 /label=OP1 promoter 2233..2238 /label=-35 promoter 2256..2261 /label=-10 promoter 2117..2273 /label=BBa_R0079 misc_feature 1374..2273 /label=BBa_K081001 RBS 2282..2296 /label=RBS-1\Strong RBS 2289..2293 /label=RBS misc_feature 2282..2296 /label=BBa_B0030 RBS 2282..2296 /label=BBa_B0030 CDS 2303..3022 /label=GFP protein CDS 2303..3022 /label=BBa_E0040 stem_loop 3042..3085 /label=stem_loop misc_feature 3031..3110 /label=BBa_B0010 terminator 3031..3110 /label=BBa_B0010 stem_loop 3126..3145 /label=T7 TE misc_feature 3152..3152 /label=stop polyA_site 3146..3159 /label=polya misc_feature 3119..3159 /label=BBa_B0012 terminator 3119..3159 /label=BBa_B0012 misc_feature 2282..3159 /label=BBa_E0840 promoter 3168..3404 /label=promoter promoter 3168..3404 /label=BBa_K823003 RBS 3413..3423 /label=RBS-4\Weaker RBS 3419..3422 /label=RBS misc_feature 3413..3423 /label=BBa_B0033 RBS 3413..3423 /label=BBa_B0033 misc_feature 4519..4557 /label=LVA CDS 3430..4557 /label=lacI-LVA misc_feature 3430..4557 /label=BBa_C0012 misc_feature 4558..4582 /label=Help:Barcodes CDS 3430..4557 /label=BBa_C0012 stem_loop 4602..4645 /label=stem_loop misc_feature 4591..4670 /label=BBa_B0010 terminator 4591..4670 /label=BBa_B0010 stem_loop 4686..4705 /label=T7 TE misc_feature 4712..4712 /label=stop polyA_site 4706..4719 /label=polya misc_feature 4679..4719 /label=BBa_B0012 terminator 4679..4719 /label=BBa_B0012 misc_feature 3413..4719 /label=BBa_P0312 ORIGIN 1 ggagttctga gaattggtat gccttataag tccaattaac agttgaaaac ctgcatagga 61 gagctatgcg ggttttttat tttacataat gatacataat ttaccgaaac ttgcggaaca 121 taattgagga atcatagaat tttgtcaaaa taattttatt gacaacgtct tattaacgtt 181 gatataattt aaattttatt tgacaaaaat gggctcgtgt tgtacaataa atgtagttac 241 tagagaaagg tggtgaatac tagatggcct tggttgacgg ttttcttgag ctggaacgct 301 caagtggaaa attggagtgg agcgccatcc tccagaagat ggcgagcgac cttggattct 361 cgaagatcct gttcggcctg ttgcctaagg acagccagga ctacgagaac gccttcatcg 421 tcggcaacta cccggccgcc tggcgcgagc attacgaccg ggctggctac gcgcgggtcg 481 acccgacggt cagtcactgt acccagagcg tactgccgat tttctgggaa ccgtccatct 541 accagacgcg aaagcagcac gagttcttcg aggaagcctc ggccgccggc ctggtgtatg 601 ggctgaccat gccgctgcat ggtgctcgcg gcgaactcgg cgcgctgagc ctcagcgtgg 661 aagcggaaaa ccgggccgag gccaaccgtt tcatagagtc ggtcctgccg accctgtgga 721 tgctcaaaga ctacgcactg caaagcggtg ccggactggc cttcgaacat ccggtcagca 781 aaccggtggt tctgaccagc cgggagaagg aagtgttgca gtggtgcgcc atcggcaaga 841 ccagttggga gatatcggtt atctgcaact gctcggaagc caatgtgaac ttccatatgg 901 gaaatattcg gcggaagttc ggtgtgacct cccgccgcgt agcggccatt atggccgtta 961 atttgggtct tattactctc tgatactaga gccaggcatc aaataaaacg aaaggctcag 1021 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt 1081 cacactggct caccttcggg tgggcctttc tgcgtttata tactagaggg agttctgaga 1141 attggtatgc cttataagtc caattaacag ttgaaaacct gcataggaga gctatgcggg 1201 ttttttattt tacataatga tacataattt accgaaactt gcggaacata attgaggaat 1261 catagaattt tgtcaaaata attttattga caacgtctta ttaacgttga tataatttaa 1321 attttatttg acaaaaatgg gctcgtgttg tacaataaat gtagttacta gagattaaag 1381 aggagaaata ctagatgatc gttcagatcg gtcgtcgtga agagttcgac aaaaaactgc 1441 tgggtgaaat gcacaaactg cgtgctcagg ttttcaaaga acgtaaaggt tgggacgttt 1501 ccgttatcga cgaaatggaa atcgacggtt acgacgctct gtccccgtac tacatgctga 1561 tccaggaaga caccccggaa gctcaggttt tcggttgctg gcgtatcttc gacaccaccg 1621 gtccgtacat gctgaaaaac accttcccgg aactgctgca cggtaaagaa gctccgtgct 1681 ccccgcacat ctgggaactg tcccgtttcg ctatcaactc cggtcagaaa ggttccctgg 1741 gtttctccga ctgcaccctg gaagctatgc gtgctctggc tcgttactcc ttgcagaacg 1801 acatccagac cctggttacc gttaccaccg ttggtgttga aaaaatgatg atccgtgctg 1861 gtctggacgt ttcccgtttc ggtccgcacc tgaaaatcgg tatcgaacgt gctgttgctc 1921 tgcgtatcga actgaacgct aaaacccaga tcgctctgta cggtggtgtt ctggttgaac 1981 agcgtctggc tgtttccgct gctaacgacg aaaactacgc tctggttgct taataactct 2041 gatagtgcta gtgtagatct ctactagaga aaaaaaaacc ccgcccctga cagggcgggg 2101 ttttttttta ctagaggccc ctcgctgagc gcgtcccgga gctgggggca acctagctgc 2161 cacctgcttt tctgctagct attccagcga aaacatacag atttccggcg aaatcaaggc 2221 tacctgccag ttctggcagg tttggccgcg ggttcttttt ggtacacgaa agctactaga 2281 gattaaagag gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc 2341 aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg 2401 tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact 2461 acctgttcca tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag 2521 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt 2581 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa 2641 gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga 2701 tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat 2761 ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga 2821 tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt 2881 ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga 2941 aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat 3001 ggatgaacta tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt 3061 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 3121 acactggctc accttcgggt gggcctttct gcgtttatat actagaggga gttctgagaa 3181 ttggtatgcc ttataagtcc aattaacagt tgaaaacctg cataggagag ctatgcgggt 3241 tttttatttt acataatgat acataattta ccgaaacttg cggaacataa ttgaggaatc 3301 atagaatttt gtcaaaataa ttttattgac aacgtcttat taacgttgat ataatttaaa 3361 ttttatttga caaaaatggg ctcgtgttgt acaataaatg tagttactag agtcacacag 3421 gactactaga tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt 3481 gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg 3541 cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa 3601 caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac 3661 gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg 3721 gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt 3781 ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt 3841 gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca 3901 cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg 3961 gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg 4021 cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg 4081 gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat 4141 gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg 4201 cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac 4261 gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc 4321 ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag 4381 ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg 4441 caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc 4501 cgactggaaa gcgggcaggc tgcaaacgac gaaaactacg ctttagtagc ttaataactc 4561 tgatagtgct agtgtagatc tctactagag ccaggcatca aataaaacga aaggctcagt 4621 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc 4681 acactggctc accttcgggt gggcctttct gcgtttata //