LOCUS       BBa_K1529263            1435 bp    DNA     linear       15-May-2024
DEFINITION  Plux-CmR-LasI
ACCESSION   BBa_K1529263
VERSION     BBa_K1529263.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     RBS             69..72
                     /label=RBS
     misc_feature    80..740
                     /label=CmR
     misc_feature    64..742
                     /label=BBa_K395160
     misc_feature    755..758
                     /label=conserved
     RBS             751..762
                     /label=BBa_B0034
     CDS             769..1371
                     /label=LasI
     misc_feature    1372..1404
                     /label=LVA
     misc_feature    1411..1435
                     /label=Help:Barcodes
     CDS             769..1435
                     /label=BBa_C0078
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagagctaag gaagctaaaa tggagaaaaa aatcacggga tataccaccg ttgatatatc
      121 ccaatggcat cgtaaagaac attttgaggc atttcagtca gttgctcaat gtacctataa
      181 ccagaccgtt cagctggata ttacggcctt tttaaagacc gtaaagaaaa ataagcacaa
      241 gttttatccg gcctttattc acattcttgc ccgcctgatg aacgctcacc cggagtttcg
      301 tatggccatg aaagacggtg agctggtgat ctgggatagt gttcaccctt gttacaccgt
      361 tttccatgag caaactgaaa cgttttcgtc cctctggagt gaataccacg acgatttccg
      421 gcagtttctc cacatatatt cgcaagatgt ggcgtgttac ggtgaaaacc tggcctattt
      481 ccctaaaggg tttattgaga atatgttttt tgtctcagcc aatccctggg tgagtttcac
      541 cagttttgat ttaaacgtgg ccaatatgga caacttcttc gcccccgttt tcacgatggg
      601 caaatattat acgcaaggcg acaaggtgct gatgccgctg gcgatccagg ttcatcatgc
      661 cgtttgtgat ggcttccatg tcggccgcat gcttaatgaa ttacaacagt actgtgatga
      721 gtggcagggc ggggcgtaat aatactagag aaagaggaga aatactagat gatcgttcag
      781 atcggtcgtc gtgaagagtt cgacaaaaaa ctgctgggtg aaatgcacaa actgcgtgct
      841 caggttttca aagaacgtaa aggttgggac gtttccgtta tcgacgaaat ggaaatcgac
      901 ggttacgacg ctctgtcccc gtactacatg ctgatccagg aagacacccc ggaagctcag
      961 gttttcggtt gctggcgtat cttcgacacc accggtccgt acatgctgaa aaacaccttc
     1021 ccggaactgc tgcacggtaa agaagctccg tgctccccgc acatctggga actgtcccgt
     1081 ttcgctatca actccggtca gaaaggttcc ctgggtttct ccgactgcac cctggaagct
     1141 atgcgtgctc tggctcgtta ctccttgcag aacgacatcc agaccctggt taccgttacc
     1201 accgttggtg ttgaaaaaat gatgatccgt gctggtctgg acgtttcccg tttcggtccg
     1261 cacctgaaaa tcggtatcga acgtgctgtt gctctgcgta tcgaactgaa cgctaaaacc
     1321 cagatcgctc tgtacggtgg tgttctggtt gaacagcgtc tggctgtttc cgctgctaac
     1381 gacgaaaact acgctctggt tgcttaataa ctctgatagt gctagtgtag atctc
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