LOCUS       BBa_K1532006            1183 bp    DNA     linear       02-Jun-2024
DEFINITION  Plac->LuxR
ACCESSION   BBa_K1532006
VERSION     BBa_K1532006.1  
FEATURES             Location/Qualifiers
     misc_feature    3..8
                     /label=Sal I cutting site
     misc_feature    1..10
                     /label=BBa_K1532001
     CDS             19..106
                     /label=end of LacI coding region (inactive)
     misc_feature    19..218
                     /label=BBa_R0010
     misc_feature    107..144
                     /label=CAP binding site
     promoter        155..160
                     /label=-35
     promoter        179..184
                     /label=-10
     misc_feature    184..218
                     /label=LacI binding site
     misc_feature    191..191
                     /label=start
     promoter        19..218
                     /label=BBa_R0010
     RBS             227..241
                     /label=RBS-1\Strong
     RBS             234..238
                     /label=RBS
     misc_feature    227..241
                     /label=BBa_B0030
     RBS             227..241
                     /label=BBa_B0030
     misc_feature    248..249
                     /label=prefix
     misc_feature    421..421
                     /label=T
     misc_feature    739..739
                     /label=A
     CDS             248..997
                     /label=luxR
     misc_feature    248..1003
                     /label=BBa_C0062
     misc_feature    1004..1028
                     /label=Help:Barcodes
     CDS             248..1003
                     /label=BBa_C0062
     stem_loop       1048..1091
                     /label=stem_loop
     misc_feature    1037..1116
                     /label=BBa_B0010
     terminator      1037..1116
                     /label=BBa_B0010
     stem_loop       1132..1151
                     /label=T7 TE
     misc_feature    1158..1158
                     /label=stop
     polyA_site      1152..1165
                     /label=polya
     misc_feature    1125..1165
                     /label=BBa_B0012
     terminator      1125..1165
                     /label=BBa_B0012
     terminator      1037..1165
                     /label=BBa_B0015
     misc_feature    1176..1181
                     /label=Sal I cutting site
     misc_feature    1174..1183
                     /label=BBa_K1532001
ORIGIN
        1 gcgtcgacgg tactagagca atacgcaaac cgcctctccc cgcgcgttgg ccgattcatt
       61 aatgcagctg gcacgacagg tttcccgact ggaaagcggg cagtgagcgc aacgcaatta
      121 atgtgagtta gctcactcat taggcacccc aggctttaca ctttatgctt ccggctcgta
      181 tgttgtgtgg aattgtgagc ggataacaat ttcacacata ctagagatta aagaggagaa
      241 atactagatg aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc
      301 ttgtagaagc aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg
      361 tgaatattat ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat
      421 cctagataat taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata
      481 tgatcctata gtagattatt ctaactccaa tcattcacca attaattgga atatatttga
      541 aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct
      601 tatcactggg tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt
      661 tgcacattca gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat
      721 accattaatt gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa
      781 atcaaacaac gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa
      841 aagctcttgg gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt
      901 aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat
      961 tttaacagga gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg
     1021 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1081 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1141 tcgggtgggc ctttctgcgt ttatatacta gaggcgtcga cgg
//