BBa_K1539002

BBa_K1539002 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1539002

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Coleen Tran

Date created: 2014-10-08 11:00:00

Date modified: 2015-05-08 01:10:52

LPromoter->LRBS->mCherry

Details

• Types: DnaRegion
• Roles: Coding; CDS
• Sequences: BBa_K1539002_sequence (Version 1)

Description

This coding sequence contains a low efficiency promoter followed by a low efficiency RBS allowing for the expression of mCherry

Notes

RBS primer construction needed to maintain the EcoRI and XbaI site. The promoter primer design could not maintain the EcoRI site but does contain the XbaI sequence. EcoRI was reestablished when the entire coding sequence was ligated to the backbone.

Source

mCherry from BBa_J06504
RBS and promoter from created primers

Sequence Annotations

Sequence Annotation	Location	Component / Role(s)
BBa_K1539089	1,35	promoter, feature/promoter
BBa_K1539034	44,56	ribosome_entry_site, feature/rbs
BBa_J06504	63,773	feature/cds, CDS

Other Properties

• igem#sampleStatus: It's complicated
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1539002/1

Member of these Collections

• iGEM Parts Registry