LOCUS       BBa_K1555003            1021 bp    DNA     linear       01-Jun-2024
DEFINITION  GFP induced by micro-aerobic promoter vgb
ACCESSION   BBa_K1555003
VERSION     BBa_K1555003.1  
FEATURES             Location/Qualifiers
     promoter        1..135
                     /label=vgb promoter
     promoter        1..135
                     /label=BBa_K561001
     RBS             144..158
                     /label=RBS-1\Strong
     RBS             151..155
                     /label=RBS
     misc_feature    144..158
                     /label=BBa_B0030
     RBS             144..158
                     /label=BBa_B0030
     CDS             165..884
                     /label=GFP protein
     CDS             165..884
                     /label=BBa_E0040
     stem_loop       904..947
                     /label=stem_loop
     misc_feature    893..972
                     /label=BBa_B0010
     terminator      893..972
                     /label=BBa_B0010
     stem_loop       988..1007
                     /label=T7 TE
     misc_feature    1014..1014
                     /label=stop
     polyA_site      1008..1021
                     /label=polya
     misc_feature    981..1021
                     /label=BBa_B0012
     terminator      981..1021
                     /label=BBa_B0012
     misc_feature    144..1021
                     /label=BBa_E0840
ORIGIN
        1 acaggacgct ggggttaaaa gtatttgagt tttgatgtgg attaagtttt aagaggcaat
       61 aaagattata ataagtgctg ctacaccata ctgatgtatg gcaaaaccat aataatgaac
      121 ttaaggaaga ccctctacta gagattaaag aggagaaata ctagatgcgt aaaggagaag
      181 aacttttcac tggagttgtc ccaattcttg ttgaattaga tggtgatgtt aatgggcaca
      241 aattttctgt cagtggagag ggtgaaggtg atgcaacata cggaaaactt acccttaaat
      301 ttatttgcac tactggaaaa ctacctgttc catggccaac acttgtcact actttcggtt
      361 atggtgttca atgctttgcg agatacccag atcatatgaa acagcatgac tttttcaaga
      421 gtgccatgcc cgaaggttat gtacaggaaa gaactatatt tttcaaagat gacgggaact
      481 acaagacacg tgctgaagtc aagtttgaag gtgataccct tgttaataga atcgagttaa
      541 aaggtattga ttttaaagaa gatggaaaca ttcttggaca caaattggaa tacaactata
      601 actcacacaa tgtatacatc atggcagaca aacaaaagaa tggaatcaaa gttaacttca
      661 aaattagaca caacattgaa gatggaagcg ttcaactagc agaccattat caacaaaata
      721 ctccaattgg cgatggccct gtccttttac cagacaacca ttacctgtcc acacaatctg
      781 ccctttcgaa agatcccaac gaaaagagag accacatggt ccttcttgag tttgtaacag
      841 ctgctgggat tacacatggc atggatgaac tatacaaata ataatactag agccaggcat
      901 caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg ttgtttgtcg
      961 gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt ctgcgtttat
     1021 a
//