LOCUS       BBa_K1555004            2904 bp    DNA     linear       19-May-2024
DEFINITION  the expression of T7 polymerase controlled by copa promoter
ACCESSION   BBa_K1555004
VERSION     BBa_K1555004.1  
FEATURES             Location/Qualifiers
     promoter        1..241
                     /label=copa promoter
     misc_feature    250..252
                     /label=start
     misc_feature    250..2904
                     /label=BBa_K145001
     CDS             253..2898
                     /label=T7 polymerase
     misc_feature    2899..2901
                     /label=stop
     misc_feature    2902..2904
                     /label=stop
ORIGIN
        1 cctttttata gatgcgggag gtaattcctc accccggtgc cgattttcag gcatcctgat
       61 ttaacttagc acccgcaact taactacagg aaaacaaaga gataaatgtc taatcctgat
      121 gcaaatcgag ccgatttttt aatctttacg gacttttacc cgcctggttt attaatttct
      181 tgaccttccc cttgctggaa ggtttaacct ttatcacagc cagtcaaaac tgtcttaaag
      241 gtactagaga tgaacacgat taacatcgct aagaacgact tctctgacat cgaactggct
      301 gctatcccgt tcaacactct ggctgaccat tacggtgagc gtttagctcg cgaacagttg
      361 gcccttgagc atgagtctta cgagatgggt gaagcacgct tccgcaagat gtttgagcgt
      421 caacttaaag ctggtgaggt tgcggataac gctgccgcca agcctctcat cactacccta
      481 ctccctaaga tgattgcacg catcaacgac tggtttgagg aagtgaaagc taagcgcggc
      541 aagcgcccga cagccttcca gttcctgcaa gaaatcaagc cggaagccgt agcgtacatc
      601 accattaaga ccactctggc ttgcctaacc agtgctgaca atacaaccgt tcaggctgta
      661 gcaagcgcaa tcggtcgggc cattgaggac gaggctcgct tcggtcgtat ccgtgacctt
      721 gaagctaagc acttcaagaa aaacgttgag gaacaactca acaagcgcgt agggcacgtc
      781 tacaagaaag catttatgca agttgtcgag gctgacatgc tctctaaggg tctactcggt
      841 ggcgaggcgt ggtcttcgtg gcataaggaa gactctattc atgtaggagt acgctgcatc
      901 gagatgctca ttgagtcaac cggaatggtt agcttacacc gccaaaatgc tggcgtagta
      961 ggtcaagact ctgagactat cgaactcgca cctgaatacg ctgaggctat cgcaacccgt
     1021 gcaggtgcgc tggctggcat ctctccgatg ttccaacctt gcgtagttcc tcctaagccg
     1081 tggactggca ttactggtgg tggctattgg gctaacggtc gtcgtcctct ggcgctggtg
     1141 cgtactcaca gtaagaaagc actgatgcgc tacgaagacg tttacatgcc tgaggtgtac
     1201 aaagcgatta acattgcgca aaacaccgca tggaaaatca acaagaaagt cctagcggtc
     1261 gccaacgtaa tcaccaagtg gaagcattgt ccggtcgagg acatccctgc gattgagcgt
     1321 gaagaactcc cgatgaaacc ggaagacatc gacatgaatc ctgaggctct caccgcgtgg
     1381 aaacgtgctg ccgctgctgt gtaccgcaag gacaaggctc gcaagtctcg ccgtatcagc
     1441 cttgagttca tgcttgagca agccaataag tttgctaacc ataaggccat ctggttccct
     1501 tacaacatgg actggcgcgg tcgtgtttac gctgtgtcaa tgttcaaccc gcaaggtaac
     1561 gatatgacca aaggactgct tacgctggcg aaaggtaaac caatcggtaa ggaaggttac
     1621 tactggctga aaatccacgg tgcaaactgt gcgggtgtcg ataaggttcc gttccctgag
     1681 cgcatcaagt tcattgagga aaaccacgag aacatcatgg cttgcgctaa gtctccactg
     1741 gagaacactt ggtgggctga gcaagattct ccgttctgct tccttgcgtt ctgctttgag
     1801 tacgctgggg tacagcacca cggcctgagc tataactgct cccttccgct ggcgtttgac
     1861 gggtcttgct ctggcatcca gcacttctcc gcgatgctcc gagatgaggt aggtggtcgc
     1921 gcggttaact tgcttcctag tgaaaccgtt caggacatct acgggattgt tgctaagaaa
     1981 gtcaacgaga ttctacaagc agacgcaatc aatgggaccg ataacgaagt agttaccgtg
     2041 accgatgaga acactggtga aatctctgag aaagtcaagc tgggcactaa ggcactggct
     2101 ggtcaatggc tggcttacgg tgttactcgc agtgtgacta agcgttcagt catgacgctg
     2161 gcttacgggt ccaaagagtt cggcttccgt caacaagtgc tggaagatac cattcagcca
     2221 gctattgatt ccggcaaggg tctgatgttc actcagccga atcaggctgc tggatacatg
     2281 gctaagctga tttgggaatc tgtgagcgtg acggtggtag ctgcggttga agcaatgaac
     2341 tggcttaagt ctgctgctaa gctgctggct gctgaggtca aagataagaa gactggagag
     2401 attcttcgca agcgttgcgc tgtgcattgg gtaactcctg atggtttccc tgtgtggcag
     2461 gaatacaaga agcctattca gacgcgcttg aacctgatgt tcctcggtca gttccgctta
     2521 cagcctacca ttaacaccaa caaagatagc gagattgatg cacacaaaca ggagtctggt
     2581 atcgctccta actttgtaca cagccaagac ggtagccacc ttcgtaagac tgtagtgtgg
     2641 gcacacgaga agtacggaat cgaatctttt gcactgattc acgactcctt cggtaccatt
     2701 ccggctgacg ctgcgaacct gttcaaagca gtgcgcgaaa ctatggttga cacatatgag
     2761 tcttgtgatg tactggctga tttctacgac cagttcgctg accagttgca cgagtctcaa
     2821 ttggacaaaa tgccagcact tccggctaaa ggtaacttga acctccgtga catcttagag
     2881 tcggacttcg cgttcgcgta ataa
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