BBa_K157008
1
BBa_K157008
Split-Venus-nYFP
2008-10-25T11:00:00Z
2015-05-08T01:10:54Z
Gene synthesis by GeneArt, optimized for expression in homo sapiens.
N-terminal fragment (Amino acids1-156) of the monomeric[5] yellow fluorescent protein variant ???Venus??? [4], designed for fusion/BiFC[1,3]. Reassembly with Part Bba_K157007 (Split-Venus-cYFP) generates the complete, working YFP ???Venus???, whereas combination with the C-terminal fragment (Part Bba_K157005) of the monomeric cyan fluorescent Protein ???CeruleanR206K??? (Part Bba_I757009) results in green fluorescence[2].See "Part Design" for references.
false
false
_232_
0
1673
9
It's complicated
false
Between BioBrick 1.0 sites this part is flanked 5' by NgoMI(=NgoMIV) and 3' AgeI(=PinAI) to facilitate in frame cloning for protein fusions.
false
iGEM Team Freiburg 2008
annotation2064750
1
N-terminal split venus YFP
range2064750
1
1
468
BBa_K157008_sequence
1
atggtgtccaagggcgaggaactgttcaccggcgtggtgcccatcctggtggagctggacggcgacgtgaacggccacaagttcagcgtgtccggcgagggcgaaggcgacgccacctacggcaagctgaccctgaagctgatctgcaccaccggcaagctgcccgtgccctggcccaccctggtgaccaccctgtacctccagtgcttcgccagataccccgaccacatgaagcagcacgatttcttcaagagcgccatgcccgagggctacgtgcaggaacggaccatcttcttcaaggacgacggcaactacaagaccagagccgaagtgaagttcgagggcgacacactggtgaaccggatcgagctgaagggcatcgacttcaaagaggacggcaatatcctgggccacaagctggaatacaactacaacagccacaacgtgtacatcaccgccgacaagcag
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z