Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Stefan Robert Marsden
Date created: 2015-09-07 11:00:00
Date modified: 2015-09-08 07:43:48
RBS
| Types | DnaRegion |
| Roles | ribosome_entry_site RBS |
| Sequences | BBa_K1583061_sequence (Version 1) |
Description
This RBS was used in our constructs Mfp5+CsgA and CsgA+Mfp3.CsgA is a protein monomer which can aggregate to form amyloid nanowires in natural biofilms taken from E.coli K-12 MG1655. Inspired by mussels, the Mfp5 (mussel foot protein) has high adhesive properties towards wet polar surfaces.
This DNA was synthesized. The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]
1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
Notes
The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.
Source
The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).The design was based on the paper "Strong underwater adhesives made by self-assembling multi-protein nanofibres".[1]
1. C.Zhong, T.Gurry, A.Cheng, J.Downey, Z.Deng, C. Stultz, T.Lu, Nature Nanotechnology, 2014, 9, 858-866.