LOCUS       BBa_K1583101             660 bp    DNA     linear       23-May-2024
DEFINITION  pRha + CsgA + His-tag
ACCESSION   BBa_K1583101
VERSION     BBa_K1583101.1  
FEATURES             Location/Qualifiers
     misc_feature    1..8
                     /label=BBa_G0002
     promoter        9..130
                     /label=pRha
     promoter        9..130
                     /label=BBa_K914003
     misc_feature    131..155
                     /label=Scar
     misc_feature    131..155
                     /label=BBa_K1583050
     RBS             156..167
                     /label=BBa_K1583008
     CDS             169..620
                     /label=CsgA
     misc_feature    621..639
                     /label=Linker
     misc_feature    640..660
                     /label=HIS-tag
     CDS             169..660
                     /label=BBa_K1583003
ORIGIN
        1 tactagagcc acaattcagc aaattgtgaa catcatcacg ttcatctttc cctggttgcc
       61 aatggcccat tttcctgtca gtaacgagaa ggtcgcgtat tcaggcgctt tttagactgg
      121 tcgtaatgaa tactagagca gcaaggaaat actagaagga ggtatataat gaaactgctg
      181 aaagtagcag caattgcagc aatcgtattc tccggtagcg ctctggcagg tgttgttcct
      241 cagtacggcg gcggcggtaa ccacggtggt ggcggtaata atagcggccc aaattctgag
      301 ctgaacattt accagtacgg tggcggtaac tctgcacttg ctctgcaaac tgatgcccgt
      361 aactctgact tgactattac ccagcatggc ggcggtaatg gtgcagatgt tggtcagggc
      421 tcagatgaca gctcaatcga tctgacccaa cgtggcttcg gtaacagcgc tactcttgat
      481 cagtggaacg gcaaaaattc tgaaatgacg gttaaacagt tcggtggtgg caacggtgca
      541 gcagttgacc agactgcatc taactcctcc gtcaacgtga ctcaggttgg ctttggtaac
      601 aacgcgaccg ctcatcagta cggcagcggt ggcagtggcc atcaccacca tcaccactaa
//