BBa_K1583050 1 BBa_K1583050 Scar 1 2015-09-03T11:00:00Z 2015-09-18T07:33:29Z This scar is part of a synthesized construct. Scar in constructs BBa_K1583100 - BBa_K1583112 false false _2000_ 24478 24478 9 false This scar originates from the part BBa_K1316015, on which the composite part BBa_K1583100 is based. false Max van 't Hof annotation2475064 1 Scar range2475064 1 1 25 BBa_K1583014 1 BBa_K1583014 CsgA missing the stop codon for creation of fusion proteins 2015-09-03T11:00:00Z 2015-09-18T07:00:29Z The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055). This part was designed to create a fusion protein of CsgA with Mfp3 (mussel foot protein). This protein has got high adhesive properties towards wet polar surfaces. Like this, an amyloid nanowire with high binding properties is created. false false _2000_ 24478 24463 9 false The stop codon was deleted to create a fusion protein. false Stefan Robert Marsden annotation2474839 1 CsgA range2474839 1 1 453 BBa_K1583015 1 BBa_K1583015 Linker in fusion protein 2015-09-07T11:00:00Z 2015-09-08T04:50:17Z The DNA was synthesized. The sequence of CsgA originates from the genomic DNA of E. coli K-12 MG1655.(http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres"[1] 1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5. A linker with the protein sequence GSGGSG was placed in between the CsgA and the affinity tag for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from engineered curli nanofibres"[1] 1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5. false false _2000_ 24463 24463 9 false We followed the design of "Programmable biofilm-based materials from engineered curli nanofibres"[1] 1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5. false Stefan Robert Marsden annotation2474933 1 Linker range2474933 1 1 18 BBa_K1583051 1 BBa_K1583051 Scar 2 2015-09-03T11:00:00Z 2015-09-18T07:34:25Z This scar is part of a synthesized construct. Scar in constructs BBa_K1583100 - BBa_K1583112 false false _2000_ 24478 24478 9 false This scar originates from the part BBa_K1316015, on which the composite part BBa_K1583100 is based. false Max van 't Hof annotation2475065 1 Scar range2475065 1 1 14 BBa_K1583102 1 BBa_K1583102 pRha + CsgA + Hydroxyapatite-affinity tag 2015-09-07T11:00:00Z 2015-09-18T11:07:01Z The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055). This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655. The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations). Upon induction with rhamnose, CsgA is expressed and exported into the extracellular space. There, the membrane protein CsgB can trigger the CsgA protein monomers to aggregate and form amyloid nanofibres. false false _2000_ 24478 24463 9 true The stop codon needed to be removed from CsgA to add the N-terminal hydroxyapatite affinity tag. false Stefan Robert Marsden, Hector Sanguesa Ferrer component2461032 1 BBa_K1583014 component2461029 1 BBa_K914003 component2461034 1 BBa_K1583060 component2461030 1 BBa_K1583050 component2461035 1 BBa_K1583051 component2461031 1 BBa_K1583008 component2461033 1 BBa_K1583015 annotation2461029 1 BBa_K914003 range2461029 1 1 122 annotation2461032 1 BBa_K1583014 range2461032 1 161 613 annotation2461034 1 BBa_K1583060 range2461034 1 632 670 annotation2461031 1 BBa_K1583008 range2461031 1 148 159 annotation2461030 1 BBa_K1583050 range2461030 1 123 147 annotation2461033 1 BBa_K1583015 range2461033 1 614 631 annotation2461035 1 BBa_K1583051 range2461035 1 671 684 BBa_K914003 1 BBa_K914003 L-rhamnose-inducible promoter (pRha) 2012-09-19T11:00:00Z 2015-05-08T01:13:45Z Amplification of the plasmid pJOE3075 (Dr. Altenbuchner). Released HQ 2013 L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose. false false _1179_ 0 13487 9 In stock true One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair. false Denis Samuylov annotation2188559 1 pRha range2188559 1 1 122 BBa_K1583008 1 BBa_K1583008 RBS 2015-09-03T11:00:00Z 2015-09-04T05:18:43Z Synthesized RBS false false _2000_ 24478 24478 9 false Based on RBS of BBa_K1316015. false Max van BBa_K1583060 1 BBa_K1583060 Hydroxyapatite affinity tag 2015-09-07T11:00:00Z 2015-09-08T04:31:43Z The sequence was obtained from the paper "Identification of a Highly Specific Hydroxyapatite-binding Peptide using Phage Display" [1] 1. M. Roy, S. Stanley, E. Amis, M. Becker, Adv. Mater., 2008, 20, 1830-1836. This part generates a peptide tag which shows high adhesive properties towards hydroxyapatite, a main component of teeth. false false _2000_ 24463 24463 9 false No specific design considerations were made. false Stefan Robert Marsden annotation2473127 1 Hydroxyapatite-affinity tag range2473127 1 1 39 BBa_K1583102_sequence 1 ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagagcagcaaggaaatactagaaggaggtatataatgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacggcagcggtggcagtggctctgtttctgttggtatgaaaccgtctccgcgtccgtaactactagaaggagg BBa_K1583060_sequence 1 tctgtttctgttggtatgaaaccgtctccgcgtccgtaa BBa_K1583051_sequence 1 ctactagaaggagg BBa_K1583015_sequence 1 ggcagcggtggcagtggc BBa_K1583014_sequence 1 atgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtac BBa_K1583050_sequence 1 tactagagcagcaaggaaatactag BBa_K1583008_sequence 1 aaggaggtatat BBa_K914003_sequence 1 ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z