BBa_K1583050
1
BBa_K1583050
Scar 1
2015-09-03T11:00:00Z
2015-09-18T07:33:29Z
This scar is part of a synthesized construct.
Scar in constructs BBa_K1583100 - BBa_K1583112
false
false
_2000_
24478
24478
9
false
This scar originates from the part BBa_K1316015, on which the composite part BBa_K1583100 is based.
false
Max van 't Hof
annotation2475064
1
Scar
range2475064
1
1
25
BBa_K1583014
1
BBa_K1583014
CsgA missing the stop codon for creation of fusion proteins
2015-09-03T11:00:00Z
2015-09-18T07:00:29Z
The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
This part was designed to create a fusion protein of CsgA with Mfp3 (mussel foot protein). This protein has got high adhesive properties towards wet polar surfaces. Like this, an amyloid nanowire with high binding properties is created.
false
false
_2000_
24478
24463
9
false
The stop codon was deleted to create a fusion protein.
false
Stefan Robert Marsden
annotation2474839
1
CsgA
range2474839
1
1
453
BBa_K1583015
1
BBa_K1583015
Linker in fusion protein
2015-09-07T11:00:00Z
2015-09-08T04:50:17Z
The DNA was synthesized. The sequence of CsgA originates from the genomic DNA of E. coli K-12 MG1655.(http://www.ncbi.nlm.nih.gov/gene/949055). The design was based on the paper "Programmable biofilm-based materials from
engineered curli nanofibres"[1]
1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
A linker with the protein sequence GSGGSG was placed in between the CsgA and the affinity tag for increased performance reasons. The design was based on the paper "Programmable biofilm-based materials from
engineered curli nanofibres"[1]
1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
false
false
_2000_
24463
24463
9
false
We followed the design of "Programmable biofilm-based materials from engineered curli nanofibres"[1]
1. Nguyen, P. Q., Botyanszki, Z., Tay, P. K. R., & Joshi, N. S. (2014). Programmable biofilm-based materials from engineered curli nanofibres. Nature communications, 5.
false
Stefan Robert Marsden
annotation2474933
1
Linker
range2474933
1
1
18
BBa_K1583051
1
BBa_K1583051
Scar 2
2015-09-03T11:00:00Z
2015-09-18T07:34:25Z
This scar is part of a synthesized construct.
Scar in constructs BBa_K1583100 - BBa_K1583112
false
false
_2000_
24478
24478
9
false
This scar originates from the part BBa_K1316015, on which the composite part BBa_K1583100 is based.
false
Max van 't Hof
annotation2475065
1
Scar
range2475065
1
1
14
BBa_K1583102
1
BBa_K1583102
pRha + CsgA + Hydroxyapatite-affinity tag
2015-09-07T11:00:00Z
2015-09-18T11:07:01Z
The DNA was synthesized. The sequence originates from the genomic DNA of E. coli K-12 MG1655 (http://www.ncbi.nlm.nih.gov/gene/949055).
This part was synthesized. The rhamnose promoter was used by the iGEM 2014 TU Delft team and originally added by the iGEM12 Paris Bettencourt team. RBS and CsgA originate from E. coli K-12 MG1655.
The nucleotides encoding for the second and third amino acid of the CsgA gene were changed to optimize synthesis success. (silent mutations).
Upon induction with rhamnose, CsgA is expressed and exported into the extracellular space. There, the membrane protein CsgB can trigger the CsgA protein monomers to aggregate and form amyloid nanofibres.
false
false
_2000_
24478
24463
9
true
The stop codon needed to be removed from CsgA to add the N-terminal hydroxyapatite affinity tag.
false
Stefan Robert Marsden, Hector Sanguesa Ferrer
component2461032
1
BBa_K1583014
component2461029
1
BBa_K914003
component2461034
1
BBa_K1583060
component2461030
1
BBa_K1583050
component2461035
1
BBa_K1583051
component2461031
1
BBa_K1583008
component2461033
1
BBa_K1583015
annotation2461029
1
BBa_K914003
range2461029
1
1
122
annotation2461032
1
BBa_K1583014
range2461032
1
161
613
annotation2461034
1
BBa_K1583060
range2461034
1
632
670
annotation2461031
1
BBa_K1583008
range2461031
1
148
159
annotation2461030
1
BBa_K1583050
range2461030
1
123
147
annotation2461033
1
BBa_K1583015
range2461033
1
614
631
annotation2461035
1
BBa_K1583051
range2461035
1
671
684
BBa_K914003
1
BBa_K914003
L-rhamnose-inducible promoter (pRha)
2012-09-19T11:00:00Z
2015-05-08T01:13:45Z
Amplification of the plasmid pJOE3075 (Dr. Altenbuchner).
Released HQ 2013
L-rhamnose-inducible promoter is capable of high-level recombinant protein expression in the presence of L-rhamnose, it is also tightly regulated in the absence of L-rhamnose by the addition of D-glucose.
false
false
_1179_
0
13487
9
In stock
true
One base pair modified (90: A -> T) to avoid EcoRI site. Mutation made in a less conserved base pair.
false
Denis Samuylov
annotation2188559
1
pRha
range2188559
1
1
122
BBa_K1583008
1
BBa_K1583008
RBS
2015-09-03T11:00:00Z
2015-09-04T05:18:43Z
Synthesized
RBS
false
false
_2000_
24478
24478
9
false
Based on RBS of BBa_K1316015.
false
Max van
BBa_K1583060
1
BBa_K1583060
Hydroxyapatite affinity tag
2015-09-07T11:00:00Z
2015-09-08T04:31:43Z
The sequence was obtained from the paper "Identification of a Highly Specific Hydroxyapatite-binding Peptide using Phage Display" [1]
1. M. Roy, S. Stanley, E. Amis, M. Becker, Adv. Mater., 2008, 20, 1830-1836.
This part generates a peptide tag which shows high adhesive properties towards hydroxyapatite, a main component of teeth.
false
false
_2000_
24463
24463
9
false
No specific design considerations were made.
false
Stefan Robert Marsden
annotation2473127
1
Hydroxyapatite-affinity tag
range2473127
1
1
39
BBa_K1583102_sequence
1
ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaatactagagcagcaaggaaatactagaaggaggtatataatgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtacggcagcggtggcagtggctctgtttctgttggtatgaaaccgtctccgcgtccgtaactactagaaggagg
BBa_K1583060_sequence
1
tctgtttctgttggtatgaaaccgtctccgcgtccgtaa
BBa_K1583051_sequence
1
ctactagaaggagg
BBa_K1583015_sequence
1
ggcagcggtggcagtggc
BBa_K1583014_sequence
1
atgaaactgctgaaagtagcagcaattgcagcaatcgtattctccggtagcgctctggcaggtgttgttcctcagtacggcggcggcggtaaccacggtggtggcggtaataatagcggcccaaattctgagctgaacatttaccagtacggtggcggtaactctgcacttgctctgcaaactgatgcccgtaactctgacttgactattacccagcatggcggcggtaatggtgcagatgttggtcagggctcagatgacagctcaatcgatctgacccaacgtggcttcggtaacagcgctactcttgatcagtggaacggcaaaaattctgaaatgacggttaaacagttcggtggtggcaacggtgctgcggttgaccagactgcatctaactcctccgtcaacgtgactcaggttggctttggtaacaacgcgaccgctcatcagtac
BBa_K1583050_sequence
1
tactagagcagcaaggaaatactag
BBa_K1583008_sequence
1
aaggaggtatat
BBa_K914003_sequence
1
ccacaattcagcaaattgtgaacatcatcacgttcatctttccctggttgccaatggcccattttcctgtcagtaacgagaaggtcgcgtattcaggcgctttttagactggtcgtaatgaa
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
James Alastair McLaughlin
Chris J. Myers
2017-03-06T15:00:00.000Z