LOCUS       BBa_K1583107            1591 bp    DNA     linear       18-May-2024
DEFINITION  pRha + CsgA with His-tag & pTet + RFP
ACCESSION   BBa_K1583107
VERSION     BBa_K1583107.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..755
                     /label=mrfp1
     misc_feature    762..786
                     /label=Help:Barcodes
     CDS             81..761
                     /label=BBa_E1010
     stem_loop       806..849
                     /label=stem_loop
     misc_feature    795..874
                     /label=BBa_B0010
     terminator      795..874
                     /label=BBa_B0010
     stem_loop       890..909
                     /label=T7 TE
     misc_feature    916..916
                     /label=stop
     polyA_site      910..923
                     /label=polya
     misc_feature    883..923
                     /label=BBa_B0012
     terminator      883..923
                     /label=BBa_B0012
     misc_feature    1..923
                     /label=BBa_I13521
     misc_feature    932..939
                     /label=BBa_G0002
     promoter        940..1061
                     /label=pRha
     promoter        940..1061
                     /label=BBa_K914003
     misc_feature    1062..1086
                     /label=Scar
     misc_feature    1062..1086
                     /label=BBa_K1583050
     RBS             1087..1098
                     /label=BBa_K1583008
     CDS             1100..1551
                     /label=CsgA
     misc_feature    1552..1570
                     /label=Linker
     misc_feature    1571..1591
                     /label=HIS-tag
     CDS             1100..1591
                     /label=BBa_K1583003
     misc_feature    932..1591
                     /label=BBa_K1583101
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggcttcct ccgaagacgt tatcaaagag ttcatgcgtt
      121 tcaaagttcg tatggaaggt tccgttaacg gtcacgagtt cgaaatcgaa ggtgaaggtg
      181 aaggtcgtcc gtacgaaggt acccagaccg ctaaactgaa agttaccaaa ggtggtccgc
      241 tgccgttcgc ttgggacatc ctgtccccgc agttccagta cggttccaaa gcttacgtta
      301 aacacccggc tgacatcccg gactacctga aactgtcctt cccggaaggt ttcaaatggg
      361 aacgtgttat gaacttcgaa gacggtggtg ttgttaccgt tacccaggac tcctccctgc
      421 aagacggtga gttcatctac aaagttaaac tgcgtggtac caacttcccg tccgacggtc
      481 cggttatgca gaaaaaaacc atgggttggg aagcttccac cgaacgtatg tacccggaag
      541 acggtgctct gaaaggtgaa atcaaaatgc gtctgaaact gaaagacggt ggtcactacg
      601 acgctgaagt taaaaccacc tacatggcta aaaaaccggt tcagctgccg ggtgcttaca
      661 aaaccgacat caaactggac atcacctccc acaacgaaga ctacaccatc gttgaacagt
      721 acgaacgtgc tgaaggtcgt cactccaccg gtgcttaata acgctgatag tgctagtgta
      781 gatcgctact agagccaggc atcaaataaa acgaaaggct cagtcgaaag actgggcctt
      841 tcgttttatc tgttgtttgt cggtgaacgc tctctactag agtcacactg gctcaccttc
      901 gggtgggcct ttctgcgttt atatactaga gtactagagc cacaattcag caaattgtga
      961 acatcatcac gttcatcttt ccctggttgc caatggccca ttttcctgtc agtaacgaga
     1021 aggtcgcgta ttcaggcgct ttttagactg gtcgtaatga atactagagc agcaaggaaa
     1081 tactagaagg aggtatataa tgaaactgct gaaagtagca gcaattgcag caatcgtatt
     1141 ctccggtagc gctctggcag gtgttgttcc tcagtacggc ggcggcggta accacggtgg
     1201 tggcggtaat aatagcggcc caaattctga gctgaacatt taccagtacg gtggcggtaa
     1261 ctctgcactt gctctgcaaa ctgatgcccg taactctgac ttgactatta cccagcatgg
     1321 cggcggtaat ggtgcagatg ttggtcaggg ctcagatgac agctcaatcg atctgaccca
     1381 acgtggcttc ggtaacagcg ctactcttga tcagtggaac ggcaaaaatt ctgaaatgac
     1441 ggttaaacag ttcggtggtg gcaacggtgc agcagttgac cagactgcat ctaactcctc
     1501 cgtcaacgtg actcaggttg gctttggtaa caacgcgacc gctcatcagt acggcagcgg
     1561 tggcagtggc catcaccacc atcaccacta a
//