LOCUS       BBa_K1583113            1270 bp    DNA     linear       23-May-2024
DEFINITION  pAra + fusion protein BFP_Spycatcher_His
ACCESSION   BBa_K1583113
VERSION     BBa_K1583113.1  
FEATURES             Location/Qualifiers
     misc_feature    1..6
                     /label=Scar
     misc_feature    1..6
                     /label=BBa_K1583054
     promoter        7..137
                     /label=promoter
     misc_feature    46..63
                     /label=AraI1
     misc_feature    67..84
                     /label=AraI2
     promoter        7..136
                     /label=BBa_K206000
     misc_feature    137..144
                     /label=BBa_G0002
     RBS             147..158
                     /label=RBS
     RBS             147..158
                     /label=BBa_K1583061
     CDS             162..860
                     /label=BFP
     CDS             162..860
                     /label=BBa_K1583017
     CDS             861..1247
                     /label=SpyCatcher
     misc_feature    1248..1265
                     /label=His-tag
     misc_feature    1266..1268
                     /label=Stop-codon
     CDS             861..1268
                     /label=BBa_K1583018
ORIGIN
        1 tactagacat tgattatttg cacggcgtca cactttgcta tgccatagca agatagtcca
       61 taagattagc ggatcctacc tgacgctttt tatcgcaact ctctactgtt tctccatacc
      121 gtttttttgg gctagctact agagaaaaag aggagaaata catgagcgaa ctgatcaaag
      181 agaacatgca catgaagctg tacatggaag gcaccgttga caaccaccac tttaagtgca
      241 cgtctgaggg tgagggtaag ccgtacgaag gcacccaaac catgcgtatc aaagttgtgg
      301 agggcggtcc actgccgttc gcttttgaca ttctggcgac cagcttcctg tacggttcca
      361 aaacgttcat taaccatact cagggcattc cggatttctt caaacagagc tttccggaag
      421 gtttcacctg ggagcgtgtc accacgtatg aagatggtgg tgtgttgacc gccacccaag
      481 atacctccct gcaagatggc tgtctgatct ataacgtgaa aattcgtggc gtcaacttta
      541 cgagcaatgg tccggtgatg cagaagaaaa ccctgggttg ggaggcgttt acggaaaccc
      601 tgtatccggc cgatggtggc ctggagggcc gtaacgacat ggcactgaag ctggttggtg
      661 gcagccattt gatcgcaaat atcaagacga cgtaccgcag caagaaaccg gcgaaaaatc
      721 tgaagatgcc gggtgtttac tatgtcgact accgtctgga acgcattaaa gaagcgaata
      781 atgagactta cgtggagcag cacgaggttg cagtcgcgcg ctattgcgac ttgcctagca
      841 agctgggtca taaactgaat gattacgaca tcccaacgac cgaaaacctg tattttcagg
      901 gcgccatggt tgatacctta tcaggtttat caagtgagca aggtcagtcc ggtgatatga
      961 caattgaaga agatagtgct acccatatta aattctcaaa acgtgatgag gacggcaaag
     1021 agttagctgg tgcaactatg gagttgcgtg attcatctgg taaaactatt agtacatgga
     1081 tttcagatgg acaagtgaaa gatttctacc tgtatccagg aaaatataca tttgtcgaaa
     1141 ccgcagcacc agacggttat gaggtagcaa ctgctattac ctttacagtt aatgagcaag
     1201 gtcaggttac tgtaaatggc aaagcaacta aaggtgacgc tcatattcat catcaccatc
     1261 accactaaat
//