BBa_K1585103

BBa_K1585103 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1585103
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Tobias Schwanemann
Date created: 2015-08-31 11:00:00
Date modified: 2015-09-01 03:08:18

Anderson Promoter with lacI binding site



Types
DnaRegion

Roles
promoter

Regulatory

Sequences BBa_K1585103_sequence (Version 1)

Description

Promoters of the Anderson Promoter Collection with lacI binding site derived from R_0010 to make them IPTG iducible.

Notes

We choosed lacI-binding-site of part BBa_R0010 because we assumed older parts would be more reliable.

The part is shipped in backbone J61002 because the functionality is directly visible by adding IPTG. RFP (E1010) should get expressed then.

design.png |600px

Source

Assambly of J23103 and lacI-binding-site of R_0010

The "lacI binding site" of Part R_0010 differs from lacO from Part BBa_J33207.

igem#experience
Fails
 
igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1585103/1