LOCUS BBa_K1585320 3671 bp DNA linear 06-May-2024 DEFINITION GlgAB for polycistronic expression ACCESSION BBa_K1585320 VERSION BBa_K1585320.1 FEATURES Location/Qualifiers misc_feature 5..8 /label=conserved RBS 1..12 /label=BBa_B0034 CDS 19..1455 /label=glgA misc_feature 180..180 /label=Replaced G with C to remove AviII site misc_feature 423..423 /label=Replaced G with C to remove AvrII site misc_feature 591..591 /label=Replaced C with T to remove EcoRI site misc_feature 987..987 /label=Replaced G with A to remove PvuII site misc_feature 1023..1023 /label=Replaced G with A to remove PstI site misc_feature 1215..1215 /label=Replaced T with C to remove AgeI site CDS 19..1455 /label=BBa_K1585300 misc_feature 1..1455 /label=BBa_K1585310 misc_feature 1468..1471 /label=conserved RBS 1464..1475 /label=BBa_B0034 CDS 1482..3671 /label=BBa_K1585301 misc_feature 1464..3671 /label=BBa_K1585311 ORIGIN 1 aaagaggaga aatactagat gcaggtttta catgtatgtt cagagatgtt cccgctgctt 61 aaaaccggcg gtctggctga tgttattggg gcattacccg cagcacaaat cgcagacggc 121 gttgacgctc gcgtactgtt gcctgcattt cccgatattc gccgtggcgt gaccgatgcc 181 caggtagtat cccgtcgtga taccttcgcc ggacatatca cgctgttgtt cggtcattac 241 aacggggttg gcatttacct gattgacgcg ccgcatctct atgatcgtcc gggaagcccg 301 tatcacgata ccaacttatt tgcctatacc gacaacgtat tgcgttttgc gctgctgggg 361 tgggttgggg cagaaatggc cagcgggctt gacccattct ggcgtcctga tgtggtgcat 421 gcccacgact ggcatgcagg ccttgcgcct gcgtatctgg cggcgcgcgg gcgtccggcg 481 aagtcggtgt ttactgtgca caacctggcc tatcaaggca tgttttatgc acatcacatg 541 aatgacatcc aattgccatg gtcattcttt aatattcatg ggctggaatt taacggacaa 601 atctctttcc tgaaggccgg tctgtactat gccgatcaca ttacggcggt cagtccaacc 661 tacgctcgcg agatcaccga accgcagttt gcctacggta tggaaggtct gttgcaacag 721 cgtcaccgtg aagggcgtct ttccggcgta ctgaacggcg tggacgagaa aatctggagt 781 ccagagacgg acttactgtt ggcctcgcgt tacacccgcg atacgttgga agataaagcg 841 gaaaataagc gccagttaca aatcgcaatg gggcttaagg ttgacgataa agtgccgctt 901 tttgcagtgg tgagccgtct gaccagccag aaaggtctcg acctggtgct ggaagcctta 961 ccgggtcttc tggagcaggg cgggcaactg gcgctactcg gcgcgggcga tccggtgctg 1021 caagaaggtt tccttgcggc ggcagcggaa taccccggtc aggtgggcgt tcagattggc 1081 tatcacgaag cattttcgca tcgcattatg ggcggcgcgg acgtcattct ggtgcccagc 1141 cgttttgaac cgtgcggctt aacgcaactt tatggattga agtacggtac gctgccgtta 1201 gtgcggcgca ccggcgggct tgctgatacg gtttctgact gttctcttga gaaccttgca 1261 gatggcgtcg ccagtgggtt tgtctttgaa gatagtaatg cctggtcgct gttacgggct 1321 attcgacgtg cttttgtact gtggtcccgt ccttcactgt ggcggtttgt gcaacgtcag 1381 gctatggcaa tggattttag ctggcaggtc gcggcgaagt cgtaccgtga gctttactat 1441 cgcttgaaat aataatacta gagaaagagg agaaatacta gatgtccgat cgtatcgata 1501 gagacgtgat taacgcgcta attgcaggcc attttgccga tcctttttcc gtactgggaa 1561 tgcataaaac caccgcggga ctggaagtcc gtgccctttt acccgacgct accgatgtgt 1621 gggtgattga accgaaaacc gggcgcaaac tcgcaaaact ggagtgtctc gactcacggg 1681 gattctttag cggcgtcatt ccgcgacgta agaatttttt ccgctatcag ttggctgttg 1741 tctggcatgg tcagcaaaac ctgattgatg atccttaccg ttttggtccg ctaatccagg 1801 aaatggatgc ctggctatta tctgaaggta ctcacctgcg cccgtatgaa accttaggcg 1861 cgcatgcaga tactatggat ggcgtcacag gtacgcgttt ctctgtctgg gctccaaacg 1921 cccgtcgggt ctcggtggtt gggcaattca actactggga cggtcgccgt cacccgatgc 1981 gcctgcgtaa agagagcggc atctgggaac tgtttatccc tggggcgcat aacggtcagc 2041 tctataaata cgagatgatt gatgccaatg gcaacttgcg tctgaagtcc gacccttatg 2101 cctttgaagc gcaaatgcgc ccggaaaccg cgtctcttat ttgcgggctg ccggaaaagg 2161 ttgtacagac tgaagaacgc aaaaaagcga atcagtttga tgcgccaatc tctatttatg 2221 aagttcacct gggttcctgg cgtcgccaca ccgacaacaa tttctggttg agctaccgcg 2281 agctggccga tcaactggtg ccttatgcta aatggatggg ctttacccac ctcgaactac 2341 tgcccattaa cgagcatccc ttcgatggca gttggggtta tcagccaacc ggcctgtatg 2401 cgccaacccg ccgttttggt actcgcgacg acttccgtta tttcattgat gccgcacacg 2461 cggctggtct gaacgtgatt ctcgactggg tgccaggcca cttcccgact gatgactttg 2521 cgcttgccga atttgatggc acgaacttgt atgaacacag cgatccgcgt gaaggctatc 2581 atcaggactg gaacacgctg atctacaact atggtcgccg tgaagtcagt aacttcctcg 2641 tcggtaacgc gctttactgg attgaacgtt ttggtattga tgcgctgcgc gtcgatgcgg 2701 tggcgtcaat gatttatcgc gactacagcc gtaaagaggg ggagtggatt ccgaacgaat 2761 ttggcgggcg cgagaatctt gaagcgattg aatttttgcg taataccaac cgtattcttg 2821 gtgagcaggt ttccggtgcg gtgacaatgg ctgaggagtc taccgatttc cctggcgttt 2881 ctcgtccgca ggatatgggc ggtctgggct tctggtacaa gtggaacctc ggctggatgc 2941 atgacaccct ggactacatg aagctcgacc cggtttatcg tcagtatcat cacgataaac 3001 tgaccttcgg gattctctac aactacactg aaaacttcgt cctgccgttg tcgcatgatg 3061 aagtggtcca cggtaaaaaa tcgattctcg accgcatgcc gggcgacgca tggcagaaat 3121 tcgcgaacct gcgcgcctac tatggctgga tgtgggcatt cccgggcaag aaactactgt 3181 tcatgggtaa cgaatttgcc cagggccgcg agtggaacca tgacgccagc ctcgactggc 3241 atctgttgga aggcggcgat aactggcacc acggtgtcca gcgtctggtg cgcgatctga 3301 acctcaccta ccgccaccat aaagcaatgc atgaactgga ttttgacccg tacggctttg 3361 aatggctggt ggtggatgac aaagaacgct cggtgctgat ctttgtgcgt cgcgataaag 3421 agggtaacga aatcatcgtt gccagtaact ttacgccggt accgcgtcat gattatcgct 3481 tcggcataaa ccagccgggc aaatggcgtg aaatcctcaa taccgattcc atgcactatc 3541 acggcagtaa tgcaggcaat ggcggcacgg tacacagcga tgagattgcc agccacggtc 3601 gtcagcattc actaagcctg acgctaccac cgctggccac tatctggctg gttcgggagg 3661 cagaataata a //