BBa_K1598003 1 BBa_K1598003 RBS-ChAT-6xHis-Terminator 2015-08-26T11:00:00Z 2015-08-28T10:29:27Z DNA synthesis This part consists of <a href="http://parts.igem.org/Part:BBa_B0034">B0034 RBS</a>, human choline acetyltransferase (CHAT) gene with C-terminal His tag, and <a href="http://parts.igem.org/Part:BBa_B0015">B0015 terminator</a>. false false _2015_ 27259 27180 9 false - human CHAT gene (UniProt accession no: P28329) was codon-optimized for E.coli and provided with C-terminal 6xHis tag - due to its length, the sequence was synthesized as two gBlock fragments with 30 bp overlap between each other and with 20 bp overlaps with respective PSB1C3 ends - the construct was cloned into PSB1C3 using Gibson Assembly false Marta Napiorkowska annotation2438652 1 RBS B0034 range2438652 1 1 12 annotation2438653 1 CHAT range2438653 1 19 1932 annotation2438654 1 Terminator B0015 range2438654 1 1941 2069 BBa_K1598003_sequence 1 aaagaggagaaatactagatggcagccaaaacccccagtagtgaagaaagcggcctgcctaaattgccagtgccgcctctgcaacagacgctggccacgtatttacagtgcatgcgtcaccttgtcagcgaggagcagtttcggaaatcccaggcaatcgttcagcagttcggcgcgccagggggcctgggtgaaacactgcaacagaaactgctggagcgccaggaaaaaactgctaactgggtttcggaatattggttaaacgatatgtacctgaataaccgtctggccctgcccgttaactcgagtcctgccgtcatcttcgcgcgccaacacttcccgggtactgatgatcagctgcgttttgcggcctctctgattagcggtgtgttgtcgtataaagccctgttggattcccatagcattccgaccgactgcgcgaaagggcagctgagcgggcagcccctgtgtatgaagcagtactatggcctgttttctagctatcgcctgcccggccacacccaagatacgctcgtggcccagaactcgtcgattatgccagagccagaacacgtgattgttgcttgctgtaatcaattttttgtcctggatgtcgtgattaatttccgccgtctcagcgagggtgatctgttcacccagctgcgcaaaattgtaaaaatggctagcaacgaagacgaacgtctgccgcctatcggcttactgacctctgatgggcgctcagaatgggcggaagcacgcaccgtgctggtcaaagattccactaatcgtgactcactggacatgatcgagcgctgcatctgcctggtctgtttagatgcaccgggtggcgttgaactctcggacactcaccgcgcgcttcagcttctgcacggcggggggtactcgaaaaatggcgcgaatcgttggtatgataaatcgctgcaattcgttgtgggacgggacgggacctgcggcgtggtctgtgaacacagcccatttgatggtattgttcttgtgcaatgcaccgagcatctgttaaaacacatgacccagagcagccgtaaactgatccgtgcagactcagtttccgagctgccggcaccgcgccgtttacgttggaaatgctcgccggaaattcaaggacacctcgcaagttcggccgaaaaattacagcgcattgtgaaaaatctcgattttattgtgtataaattcgataattacgggaagaccttcattaaaaagcaaaaatgttcgcctgatgcgttcattcaagtcgcgttacagctggcgttctatcgcctccatcggcgtctcgtgccaacctacgagagcgcttcaatccgccgctttcaggaaggtcgtgtagataacattcgttcggccacccctgaagctcttgcttttgtgcgcgctgttacggaccacaaagctgccgtccccgcgagcgaaaaattactgctgctgaaagacgccattcgcgcgcagacagcttacacggttatggccattacgggaatggctattgacaaccatctgctcgccctgcgggaactcgcccgcgctatgtgtaaggaactgccagaaatgtttatggacgaaacgtatttgatgagcaaccgttttgtgctcagcactagccaggtaccgacgactactgaaatgttctgttgttatggcccggttgttcccaacgggtatggcgcctgctacaatccgcaacccgagacgatcctgttttgtatcagtagtttccattcttgcaaagagacctcgagcagtaaatttgcgaaggccgtggaggagtctctgatcgatatgcgtgatctgtgttccctgctgcctccgacggaaagcaaaccgctcgcaaccaaagaaaaggccacgcgtccgtcccagggccatcagccacatcatcaccatcaccactaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z