BBa_K1598004

BBa_K1598004 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1598004
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Marta Napiorkowska
Date created: 2015-08-26 11:00:00
Date modified: 2015-08-27 03:11:46

RBS-GAD-6xHis-Terminator



    • Details

    Types
    DnaRegion

    Roles
    engineered_region

    Generator

    Sequences BBa_K1598004_sequence (Version 1)

    Description


    This part consists of B0034 RBS, human glutamate decarboxylase (GAD) gene with C-terminal His tag, and B0015 terminator.


    Notes


    - human CHAT gene (UniProt accession no: Q99259) was codon-optimized for E.coli and provided with C-terminal 6xHis tag

    - illegal restriction enzyme sites were removed

    - part was obtained through de novo DNA synthesis




    Source

    DNA synthesis

    Sequence Annotation Location Component / Role(s)
    RBS B0034
    GAD
    Terminator B0015
    		1,12
    19,1440
    1447,1575
    		feature/rbs ribosome_entry_site
    CDS feature/cds
    feature/stop stop_codon

    igem#status
    Planning
     
    synbiohub#ownedBy
    user/james
     
    synbiohub#ownedBy
    user/myers
     
    synbiohub#topLevel
    BBa_K1598004/1
     

    Attachments

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