BBa_K1598004
1
BBa_K1598004
RBS-GAD-6xHis-Terminator
2015-08-26T11:00:00Z
2015-08-27T03:11:46Z
DNA synthesis
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This part consists of <a href="http://parts.igem.org/Part:BBa_B0034">B0034 RBS</a>, human glutamate decarboxylase (GAD) gene with C-terminal His tag, and <a href="http://parts.igem.org/Part:BBa_B0015">B0015 terminator</a>.
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false
false
_2015_
27180
27180
9
false
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- human CHAT gene (UniProt accession no: Q99259) was codon-optimized for E.coli and provided with C-terminal 6xHis tag<br>
- illegal restriction enzyme sites were removed <br>
- part was obtained through de novo DNA synthesis
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false
Marta Napiorkowska
annotation2438657
1
Terminator B0015
range2438657
1
1447
1575
annotation2438655
1
RBS B0034
range2438655
1
1
12
annotation2438656
1
GAD
range2438656
1
19
1440
BBa_K1598004_sequence
1
aaagaggagaaatactagatggaccagaagctgttaacggatttccgctcagaactactcgattcacgttttggcgcaaaggccatttctactatcgcggagtcaaaacgatttccgctgcacgaaatgcgcgatgatgtcgcatttcagattatcaatgatgaattatatcttgatggcaacgctcgtcagaacctggccactttctgccagacctgggacgacgaaaacgtccataaattgatggatttgtcgatcaataaaaactggatcgacaaagaagaatatccgcaatccgcagccatcgacctgcgttgcgtaaatatggttgccgatctgtggcatgcgcctgcgccgaaaaatggtcaggccgttggcaccaacaccattggttcttccgaggcctgtatgctcggcgggatggcgatgaaatggcgttggcgcaagcgtatggaagctgcgggcaaaccaacggataaaccaaacctggtgtgcggtccggtacaaatctgctggcataaattcgcccgctactgggatgtggagctgcgtgagatccctatgcgccccggtcagttgtttatggacccgaaacgcatgattgaagcctgtgacgaaaacaccatcggcgtggtgccgactttcggcgtgacctacaccggtaactatgagttcccacaaccgctgcacgatgcgctggataaattccaggccgacaccggtatcgacatcgacatgcacatcgacgctgccagcggtggcttcctggcaccgttcgtcgccccggatatcgtctgggacttccgcctgccgcgtgtgaaatcgatcagtgcttcaggccataaattcggtctggctccgctgggctgcggctgggttatctggcgtgacgaagaagcgctgccgcaggaactggtgttcaacgttgactacctgggtggtcaaattggtacttttgccatcaacttctcccgcccggcgggtcaggtaattgcacagtactatgaatttctgcgcctcggtcgtgaaggctataccaaagtacagaacgcctcttaccaggttgccgcttatctggcggatgaaatcgccaaactggggccgtatgagttcatctgtacgggtcgcccggacgaaggcatcccggcggtttgcttcaaactgaaagatggtgaagatccgggatacaccctgtacgacctctctgaacgtctgcgtctgcgcggctggcaggttccggccttcactctcggcggtgaagccaccgacatcgtggtgatgcgcattatgtgtcgtcgcggcttcgaaatggactttgctgaactgttgctggaagactacaaagcctccctgaaatatctcagcgatcacccgaaactgcaaggtattgcccagcagaacagctttaaacacacccatcatcaccatcaccactaataatactagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata
igem2sbol
1
iGEM to SBOL conversion
Conversion of the iGEM parts registry to SBOL2.1
Chris J. Myers
James Alastair McLaughlin
2017-03-06T15:00:00.000Z