BBa_K1598007 1 BBa_K1598007 J23101 promoter+RBS+FDXR+RBS+FDX1+ RBS + CYP11A1 gene+rrnb double terminator 2015-09-17T11:00:00Z 2015-09-18T09:09:05Z http://www.ncbi.nlm.nih.gov/nuccore/153218645?report=fasta http://www.ncbi.nlm.nih.gov/nuccore/16877631?report=fasta http://www.ncbi.nlm.nih.gov/nuccore/39645178?report=fasta http://parts.igem.org/cgi/partsdb/puttext.cgi http://parts.igem.org/cgi/partsdb/puttext.cgi Also called Cytochrome P450scc, Cholesterol Side Chain Cleavage Enzyme (P450scc) is a mitochondrial enzyme that converts cholesterol into pregnenolone, which is a neurosteroid as well as the precursor to a variety of steroid hormones and neurotransmitters. The gene that encodes it is CYP11A1. Furthermore, the catalysis involves 3 monooxygenase reactions, which requires two electron transport proteins: ferrodoxin (FDX1) and ferredoxin reductase (FDXR). Our biobrick consists of the iGEM prefix followed by the J23101 constitutive promoter, RBS, FDXR gene, RBS, FDX1 GENE, RBS, CYP11A1 gene, rrnb double terminator, and the iGEM suffix. This will be ligated onto a PSB1C3 backbone. Emerging preclinical and clinical evidence suggests that pregnenolone may be a promising novel therapeutic candidate in schizophrenia. It has also been shown to reduce the side effects of benzodiazepines. Furthermore, cholesterol side chain cleavage is the 1st step in the biosynthesis of steroids such as testosterone, progesterone, cortisol, estrogen, etc. Therefore, this biobrick has a plethora of applications, and is in a way several biobricks in 1. true false _2015_ 27259 27256 9 false We had to make sure that that the genes for Cytochrome P450scc and all it's electron transport proteins were included in the biobrick and came after ribosome binding sites. The biobrick was broken into 5 parts for quick synthesize and assembled using Golden Gate assembly. Hence, each part was made compatible for golden gate assembly, and had overhangs for SapI. false Yash Mishra annotation2475238 1 BBa_J23101 range2475238 1 1 35 BBa_K1598007_sequence 1 tactagaaagaggagaaatactaggaattcgcggccgcttctagagatggcttcaagatgttggagatggtggggctggtcggcatggccgcgaacacggctcccacccgctggatcgacccctagtttctgccaccacttctcaacccaggagaagactcctcaaatatgtgtcgtagggtcaggccccgcaggattttacaccgctcagcatcttctcaagcatccacaagcccatgtagatatttatgaaaagcaacccgtaccgttcggattggtccgtttcggtgttgctccagaccacccagaagtaaaaaatgtcatcaataccttcactcagacggcgcattcgggccgatgtgcgttttgggggaatgtggaggtcgggcgggacgtaacagtacccgagctacaggaggcatatcatgcagtcgtcctgtcatatggggcagaagaccaccgtgcactcgaaatccctggagaggagctccctggagtatgctcagcgcgcgccttcgttgggtggtataatgggttgccagaaaaccaggaactcgagcccgatctttcttgtgacacagctgttattctaggacagggcaatgtcgcgttagacgttgcccgcatcctcctaaccccgccagagcacttagaacggacggacatcacaaaggccgcattgggcgttctccgccaaagtagagttaagactgtctggctagttggcagaaggggtccgctgcaagtggcttttacgattaaagaactccgcgaaatgatacaactcccaggcgcccgcccgattttagaccccgtggacttcttgggcctgcaagataagatcaaagaggtgccccgtcccaggaaacgcctcacggagttactcctacgtacggccaccgaaaaacctggaccggcagaagcggcgcggcaagcctccgcatcgcgcgcctggggtttacgcttttttaggagcccccagcaagtccttccgtctcctgatgggcggagagcagcgggggtgaggctcgcagtgacaagattagaaggagtcgatgaagcaactagggcagtgccgaccggtgatatggaggacttaccctgtgggcttgtattatcgtccattggttataaatctcgtcctgtcgatccgtccgtaccattcgactccaagttaggggttatacccaacgtggagggcagagtcatggacgtacccggcttatactgttcgggctgggtgaagaggggcccaactggagtcatagcgacaactatgacagatagttttcttacaggacaaatgctattacaagatttgaaggctggtttgttacctagcggccctcgccccggatatgctgcgatccaagcgttactttccagtcgcggcgtgcgtccagtatccttctccgactgggaaaagctcgacgcagaggaagtggctcgagggcaggggaccggaaaacctagggaaaagctggtcgatccacaagagatgttaaggctcttagggcactaggtttcttcaaagaggagaaatactagatggccgcagcggggggagctagactgttacgagctgcgagtgcagtcttaggtgggcccgcggggcgttggctacatcatgccgggtccagagccggctcatcggggctactccgtaaccgcggtccaggagggtctgctgaggcgagcagatcgttaagcgtaagcgcccgggcaagaagctcaagtgaggacaaaataacggtccatttcataaaccgtgatggggaaactctcacaacgaaaggtaaggtgggggactctctcttagatgtcgtcgttgagaacaacttggatattgatggatttggtgcctgtgaaggtacattagcctgctctacctgtcatttgatattcgaggaccacatctacgagaaactagacgctatcactgacgaagaaaacgacatgcttgatttagcgtatggccttacggaccgaagtcggctaggatgtcaaatttgtcttacgaagtcaatggacaacatgacagtccgtgttcctgaaacggttgctgatgcacgccagtcgatagacgtagggaaaacgagctaggtttcttcaaagaggagaaatactagatgctcgctaagggattaccgccgcgaagtgtcctcgttaaggggtgccaaacgtttctgtctgctccaagagaaggattagggaggctgagggtccccaccggagaaggcgctggtatctctacccgatctcctcgccccttcaacgagattccgtctcccggtgataacgggtggctcaatttgtatcacttctggcgcgaagaaggaacgcacaaggtgcaccttcaccatgtccagaatttccagaaatatggccccatttatcgagagaaattaggaaatgtcgaatccgtgtatgttatcgatcccgaagatgtggcattgctgtttaagtctgagggccctaatcctgaacggttcctaatcccgccatgggtcgcgtatcaccaatactatcagcgtccgatcggggtcctcctcaaaaaatccgctgcgtggaagaaagatagggtagctctgaatcaagaagtcatggccccggaggctacaaaaaatttcctcccgttgctagacgcagtgagtcgtgacttcgtgtcagtgttacaccgtaggataaagaaagcgggatctggtaattactccggtgatatttcagatgacctgttccggtttgcgttcgagagcattacaaacgtgatttttggcgaacgtcaaggaatgttagaagaggtcgtaaacccggaggcacaacggtttatcgatgctatctaccaaatgtttcatacatccgtaccgatgctgaacctccctcccgacctgttccggctttttaggacgaaaacgtggaaagatcatgtagctgcgtgggacgtgatattctctaaggctgatatttacacgcagaacttctactgggaactccgacaaaaaggttccgttcaccacgactatcgcgggatactttatcggttattgggtgacagcaaaatgtctttcgaggatataaaggcgaatgtaacggagatgttggccgggggggtcgatacaacatccatgactctccagtggcatctctatgagatggctcgaaacctcaaggttcaggatatgctccgagccgaagtgttggcggcccgacaccaggctcaaggtgacatggcgacaatgcttcagctagtgccacttctcaaggctagcattaaggaaacactcaggcttcatcctatatcagtaacattgcaacgttacctagtgaatgacttggtgctaagagactatatgattcccgccaagacacttgtccaagtggcgatatacgcactcggtcgagagccaaccttcttcttcgatcctgagaactttgatccaacgcggtggctctctaaagataagaacatcacttactttcgaaacttgggattcggttggggggttagacaatgcctcggccgtcgaatcgctgagctggagatgactatcttcctgataaacatgctcgaaaatttcagggtagaaattcagcacctgtcggatgtagggacgacctttaacctgatcctgatgccagagaagccaatctcttttacgttttggccgtttaatcaggaagctactcaacaatgaccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z