BBa_K1598008 1 CYP11A1 J23101 promoter + RBS + Human FDXR + RBS + Human FDX1 + RBS + Human CYP11A1 + rrnb double terminator 2015-09-17T11:00:00Z 2015-09-21T03:58:55Z http://www.ncbi.nlm.nih.gov/nuccore/153218645?report=fasta http://www.ncbi.nlm.nih.gov/nuccore/16877631?report=fasta http://www.ncbi.nlm.nih.gov/nuccore/39645178?report=fasta http://parts.igem.org/cgi/partsdb/puttext.cgi http://parts.igem.org/cgi/partsdb/puttext.cgi Also called Cytochrome P450scc, Cholesterol Side Chain Cleavage Enzyme (P450scc) is a mitochondrial enzyme that converts cholesterol into pregnenolone, which is a neurosteroid as well as the precursor to a variety of steroid hormones and neurotransmitters. The gene that encodes it is CYP11A1. Furthermore, the catalysis involves 3 monooxygenase reactions, which requires two electron transport proteins: ferrodoxin (FDX1) and ferredoxin reductase (FDXR). Our biobrick consists of the iGEM prefix followed by the J23101 constitutive promoter, RBS, FDXR gene, RBS, FDX1 GENE, RBS, CYP11A1 gene, rrnb double terminator, and the iGEM suffix. This will be ligated onto a PSB1C3 backbone. Emerging preclinical and clinical evidence suggests that pregnenolone may be a promising novel therapeutic candidate in schizophrenia. It has also been shown to reduce the side effects of benzodiazepines. Furthermore, cholesterol side chain cleavage is the 1st step in the biosynthesis of steroids such as testosterone, progesterone, cortisol, estrogen, etc. Therefore, this biobrick has a plethora of applications, and is in a way several biobricks in 1. false false _2015_ 27256 27256 9 true We had to make sure that that the genes for Cytochrome P450scc and all it's electron transport proteins were included in the biobrick and came after ribosome binding sites. The biobrick was broken into 5 parts for quick synthesize and assembled using Golden Gate assembly. Hence, each part was made compatible for golden gate assembly, and had overhangs for SapI. false Yash Mishra annotation2476244 1 rrnb Double Terminator preceded by some random bases range2476244 1 3717 3910 annotation2476241 1 BBa_J34801 range2476241 1 1543 1555 annotation2476243 1 Human CYP11A1 Gene range2476243 1 2151 3716 annotation2476237 1 Human FDX1 Gene range2476237 1 1562 2117 annotation2476240 1 BBa_J34801 range2476240 1 39 51 annotation2476239 1 Human FDXR Gene range2476239 1 58 1534 annotation2476238 1 BBa_J23101 range2476238 1 1 33 annotation2476242 1 BBa_J34801 range2476242 1 2126 2138 BBa_K1598008_sequence 1 tttaccgcgtcatcagtactgggaataatgctgtactagaaagaggagaaatactagatggcttcaagatgttggagatggtggggctggtcggcatggccgcgaacacggctcccacccgctggatcgacccctagtttctgccaccacttctcaacccaggagaagactcctcaaatatgtgtcgtagggtcaggccccgcaggattttacaccgctcagcatcttctcaagcatccacaagcccatgtagatatttatgaaaagcaacccgtaccgttcggattggtccgtttcggtgttgctccagaccacccagaagtaaaaaatgtcatcaataccttcactcagacggcgcattcgggccgatgtgcgttttgggggaatgtggaggtcgggcgggacgtaacagtacccgagctacaggaggcatatcatgcagtcgtcctgtcatatggggcagaagaccaccgtgcactcgaaatccctggagaggagctccctggagtatgctcagcgcgcgccttcgttgggtggtataatgggttgccagaaaaccaggaactcgagcccgatctttcttgtgacacagctgttattctaggacagggcaatgtcgcgttagacgttgcccgcatcctcctaaccccgccagagcacttagaacggacggacatcacaaaggccgcattgggcgttctccgccaaagtagagttaagactgtctggctagttggcagaaggggtccgctgcaagtggcttttacgattaaagaactccgcgaaatgatacaactcccaggcgcccgcccgattttagaccccgtggacttcttgggcctgcaagataagatcaaagaggtgccccgtcccaggaaacgcctcacggagttactcctacgtacggccaccgaaaaacctggaccggcagaagcggcgcggcaagcctccgcatcgcgcgcctggggtttacgcttttttaggagcccccagcaagtccttccgtctcctgatgggcggagagcagcgggggtgaggctcgcagtgacaagattagaaggagtcgatgaagcaactagggcagtgccgaccggtgatatggaggacttaccctgtgggcttgtattatcgtccattggttataaatctcgtcctgtcgatccgtccgtaccattcgactccaagttaggggttatacccaacgtggagggcagagtcatggacgtacccggcttatactgttcgggctgggtgaagaggggcccaactggagtcatagcgacaactatgacagatagttttcttacaggacaaatgctattacaagatttgaaggctggtttgttacctagcggccctcgccccggatatgctgcgatccaagcgttactttccagtcgcggcgtgcgtccagtatccttctccgactgggaaaagctcgacgcagaggaagtggctcgagggcaggggaccggaaaacctagggaaaagctggtcgatccacaagagatgttaaggctcttagggcactaggtttcttcaaagaggagaaatactagatggccgcagcggggggagctagactgttacgagctgcgagtgcagtcttaggtgggcccgcggggcgttggctacatcatgccgggtccagagccggctcatcggggctactccgtaaccgcggtccaggagggtctgctgaggcgagcagatcgttaagcgtaagcgcccgggcaagaagctcaagtgaggacaaaataacggtccatttcataaaccgtgatggggaaactctcacaacgaaaggtaaggtgggggactctctcttagatgtcgtcgttgagaacaacttggatattgatggctttggtgcctgcgaaggtacattagcctgctctacctgtcatttgatattcgaggaccacatctacgagaaactagacgctatcactgacgaagaaaacgacatgcttgatttagcgtatggccttacggaccgaagtcggctaggatgtcaaatttgtcttacgaagtcaatggacaacatgacagtccgtgttcctgaaacggttgctgatgcacgccagtcgatagacgtagggaaaacgagctaggtttcttcaaagaggagaaatactagatgctcgctaagggattaccgccgcgaagtgtcctcgttaaggggtgccaaacgtttctgtctgctccaagagaaggattagggaggctgagggtccccaccggagaaggcgctggtatctctacccgatctcctcgccccttcaacgagattccgtctcccggtgataacgggtggctcaatttgtatcacttctggcgcgaagaaggaacgcacaaggtgcaccttcaccatgtccagaatttccagaaatatggccccatttatcgagagaaattaggaaatgtcgaatccgtgtatgttatcgatcccgaagatgtggcattgctgtttaagtctgagggccctaatcctgaacggttcctaatcccgccatgggtcgcgtatcaccaatactatcagcgtccgatcggggtcctcctcaaaaaatccgctgcgtggaagaaagatagggtagctctgaatcaagaagtcatggccccggaggctacaaaaaatttcctcccgttgctagacgcagtgagtcgtgacttcgtgtcagtgttacaccgtaggataaagaaagcgggatctggtaattactccggtgatatttcagatgacctgttccggtttgcgttcgagagcattacaaacgtgatttttggcgaacgtcaaggaatgttagaagaggtcgtaaacccggaggcacaacggtttatcgatgctatctaccaaatgtttcatacatccgtaccgatgctgaacctccctcccgacctgttccggctttttaggacgaaaacgtggaaagatcatgtagctgcgtgggacgtgatattctctaaggctgatatttacacgcagaacttctactgggaactccgacaaaaaggttccgttcaccacgactatcgcgggatactttatcggttattgggtgacagcaaaatgtctttcgaggatataaaggcgaatgtaacggagatgttggccgggggggtcgatacaacatccatgactctccagtggcatctctatgagatggctcgaaacctcaaggttcaggatatgctccgagccgaagtgttggcggcccgacaccaggctcaaggtgacatggcgacaatgcttcagctagtgccacttctcaaggctagcattaaggaaacactcaggcttcatcctatatcagtaacattgcaacgttacctagtgaatgacttggtgctaagagactatatgattcccgccaagacacttgtccaagtggcgatatacgcactcggtcgagagccaaccttcttcttcgatcctgagaactttgatccaacgcggtggctctctaaagataagaacatcacttactttcgaaacttgggattcggttggggggttagacaatgcctcggccgtcgaatcgctgagctggagatgactatcttcctgataaacatgctcgaaaatttcagggtagaaattcagcacctgtcggatgtagggacgacctttaacctgatcctgatgccagagaagccaatctcttttacgttttggccgtttaatcaggaagctactcaacaatgataactcgagccccaagggcgacaccccctaattagcccgggcgaaaggcccagtctttcgactgagcctttcgttttatttgatgcctggcagttccctactctcgcatggggagtccccacactaccatcggcgctacggcgtttcacttctgagttcggcatggggtcaggtgggaccaccgcgctactgccgccaggcaaa igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z