LOCUS       BBa_K1600002            2942 bp    DNA     linear       07-May-2024
DEFINITION  Promoter+RBS+SacB+RBS+GlgC+Terminator
ACCESSION   BBa_K1600002
VERSION     BBa_K1600002.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23100
     misc_feature    48..51
                     /label=conserved
     RBS             44..55
                     /label=BBa_B0034
     misc_feature    1067..1069
                     /label=G336D
     misc_feature    632..637
                     /label=EcoRI site removed
     misc_feature    1129..1134
                     /label=EcoRI site removed
     CDS             62..1360
                     /label=BBa_K118016
     RBS             1369..1371
                     /label=RBS
     CDS             1381..2802
                     /label=Coding sequence
     misc_feature    1579..1584
                     /label=Removed EcoRI site
     misc_feature    1369..2805
                     /label=BBa_K322921
     stem_loop       2825..2868
                     /label=stem_loop
     misc_feature    2814..2893
                     /label=BBa_B0010
     terminator      2814..2893
                     /label=BBa_B0010
     stem_loop       2909..2928
                     /label=T7 TE
     misc_feature    2935..2935
                     /label=stop
     polyA_site      2929..2942
                     /label=polya
     misc_feature    2902..2942
                     /label=BBa_B0012
     terminator      2902..2942
                     /label=BBa_B0012
     terminator      2814..2942
                     /label=BBa_B0015
ORIGIN
        1 ttgacggcta gctcagtcct aggtacagtg ctagctacta gagaaagagg agaaatacta
       61 gatggttagt ttagagaaga acgatcactt aatgttggcg cgccagctgc cattgaaatc
      121 tgttgccctg atactggcgg gaggacgtgg tacccgcctg aaggatttaa ccaataagcg
      181 agcaaaaccg gccgtacact tcggcggtaa gttccgcatt atcgactttg cgctgtctaa
      241 ctgcatcaac tccgggatcc gtcgtatggg cgtgatcacc cagtaccagt cccacactct
      301 ggtgcagcac attcagcgcg gctggtcatt cttcaatgaa gaaatgaacg agtttgtcga
      361 tctgctgcca gcacagcaga gaatgaaagg ggaaaactgg tatcgcggca ccgcagatgc
      421 ggtcacccaa aacctcgaca ttatccgccg ttataaagcg gaatacgtgg tgatcctggc
      481 gggcgaccat atctacaagc aagactactc gcgtatgctt atcgatcacg tcgaaaaagg
      541 cgcacgttgc accgttgctt gtatgccagt accgattgaa gaagcctccg catttggcgt
      601 tatggcggtt gatgagaacg ataaaattat cgaatttgtt gaaaaacctg ctaacccgcc
      661 gtcaatgccg aacgatccga gcaaatctct ggcgagtatg ggtatctacg tctttgacgc
      721 cgactatctg tatgaactgc tggaagaaga cgatcgcgat gagaactcca gccacgactt
      781 tggcaaagat ttgattccca agatcaccga agccggtctg gcctatgcgc acccgttccc
      841 gctctcttgc gtacaatccg acccggatgc cgagccgtac tggcgcgatg tgggtacgct
      901 ggaagcttac tggaaagcga acctcgatct ggcctctgtg gtgccggaac tggatatgta
      961 cgatcgcaat tggccaattc gcacctacaa tgaatcatta ccgccagcga aattcgtgca
     1021 ggatcgctcc ggtagccacg ggatgaccct taactcactg gtttccgacg gttgtgtgat
     1081 ctccggttcg gtggtggtgc agtccgttct gttctcgcgc gttcgcgtga actcattctg
     1141 caacattgat tccgccgtat tgttaccgga agtatgggta ggtcgctcgt gccgtctgcg
     1201 ccgctgcgtc atcgatcgtg cttgtgttat tccggaaggc atggtgattg gtgaaaacgc
     1261 agaggaagat gcacgtcgtt tctatcgttc agaagaaggc atcgtgctgg taacgcgcga
     1321 aatgctacgg aagttagggc ataaacagga gcgataataa tactagagga gacatgaacg
     1381 atgaacatca aaaagtttgc aaaacaagca acagtattaa cctttactac cgcactgctg
     1441 gcaggaggcg caactcaagc gtttgcgaaa gaaacgaacc aaaagccata taaggaaaca
     1501 tacggcattt cccatattac acgccatgat atgctgcaaa tccctgaaca gcaaaaaaat
     1561 gaaaaatatc aagttcctga gttcgattcg tccacaatta aaaatatctc ttctgcaaaa
     1621 ggcctggacg tttgggacag ctggccatta caaaacgctg acggcactgt cgcaaactat
     1681 cacggctacc acatcgtctt tgcattagcc ggagatccta aaaatgcgga tgacacatcg
     1741 atttacatgt tctatcaaaa agtcggcgaa acttctattg acagctggaa aaacgctggc
     1801 cgcgtcttta aagacagcga caaattcgat gcaaatgatt ctatcctaaa agaccaaaca
     1861 caagaatggt caggttcagc cacatttaca tctgacggaa aaatccgttt attctacact
     1921 gatttctccg gtaaacatta cggcaaacaa acactgacaa ctgcacaagt taacgtatca
     1981 gcatcagaca gctctttgaa catcaacggt gtagaggatt ataaatcaat ctttgacggt
     2041 gacggaaaaa cgtatcaaaa tgtacagcag ttcatcgatg aaggcaacta cagctcaggc
     2101 gacaaccata cgctgagaga tcctcactac gtagaagata aaggccacaa atacttagta
     2161 tttgaagcaa acactggaac tgaagatggc taccaaggcg aagaatcttt atttaacaaa
     2221 gcatactatg gcaaaagcac atcattcttc cgtcaagaaa gtcaaaaact tctgcaaagc
     2281 gataaaaaac gcacggctga gttagcaaac ggcgctctcg gtatgattga gctaaacgat
     2341 gattacacac tgaaaaaagt gatgaaaccg ctgattgcat ctaacacagt aacagatgaa
     2401 attgaacgcg cgaacgtctt taaaatgaac ggcaaatggt acctgttcac tgactcccgc
     2461 ggatcaaaaa tgacgattga cggcattacg tctaacgata tttacatgct tggttatgtt
     2521 tctaattctt taactggccc atacaagccg ctgaacaaaa ctggccttgt gttaaaaatg
     2581 gatcttgatc ctaacgatgt aacctttact tactcacact tcgctgtacc tcaagcgaaa
     2641 ggaaacaatg tcgtgattac aagctatatg acaaacagag gattctacgc agacaaacaa
     2701 tcaacgtttg cgccaagctt cctgctgaac atcaaaggca agaaaacatc tgttgtcaaa
     2761 gacagcatcc ttgaacaagg acaattaaca gttaacaaat aataatacta gagccaggca
     2821 tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc
     2881 ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta
     2941 ta
//