LOCUS       BBa_K1605019             927 bp    DNA     linear       12-May-2024
DEFINITION  Ptet mRFP hesB RBS
ACCESSION   BBa_K1605019
VERSION     BBa_K1605019.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     RBS             63..78
                     /label=BBa_K1605018
     CDS             85..759
                     /label=mrfp1
     misc_feature    766..790
                     /label=Help:Barcodes
     CDS             85..790
                     /label=BBa_E1010
     stem_loop       810..853
                     /label=stem_loop
     misc_feature    799..878
                     /label=BBa_B0010
     terminator      799..878
                     /label=BBa_B0010
     stem_loop       894..913
                     /label=T7 TE
     misc_feature    920..920
                     /label=stop
     polyA_site      914..927
                     /label=polya
     misc_feature    887..927
                     /label=BBa_B0012
     terminator      887..927
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agttaaagga tttaacccta ctagatggct tcctccgaag acgttatcaa agagttcatg
      121 cgtttcaaag ttcgtatgga aggttccgtt aacggtcacg agttcgaaat cgaaggtgaa
      181 ggtgaaggtc gtccgtacga aggtacccag accgctaaac tgaaagttac caaaggtggt
      241 ccgctgccgt tcgcttggga catcctgtcc ccgcagttcc agtacggttc caaagcttac
      301 gttaaacacc cggctgacat cccggactac ctgaaactgt ccttcccgga aggtttcaaa
      361 tgggaacgtg ttatgaactt cgaagacggt ggtgttgtta ccgttaccca ggactcctcc
      421 ctgcaagacg gtgagttcat ctacaaagtt aaactgcgtg gtaccaactt cccgtccgac
      481 ggtccggtta tgcagaaaaa aaccatgggt tgggaagctt ccaccgaacg tatgtacccg
      541 gaagacggtg ctctgaaagg tgaaatcaaa atgcgtctga aactgaaaga cggtggtcac
      601 tacgacgctg aagttaaaac cacctacatg gctaaaaaac cggttcagct gccgggtgct
      661 tacaaaaccg acatcaaact ggacatcacc tcccacaacg aagactacac catcgttgaa
      721 cagtacgaac gtgctgaagg tcgtcactcc accggtgctt aataacgctg atagtgctag
      781 tgtagatcgc tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg
      841 cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac actggctcac
      901 cttcgggtgg gcctttctgc gtttata
//