BBa_K1606029

BBa_K1606029 Version 1

Component

Source: http://parts.igem.org/Part:BBa_K1606029

Generated By: https://synbiohub.org/public/igem/igem2sbol/1

Created by: Dorukcan ??zk??se

Date created: 2015-08-26 11:00:00

Date modified: 2015-08-28 04:23:36

Gluten Updated m-cherry reporter gene

Details

• Types: DnaRegion
• Roles: Reporter; engineered_region
• Sequences: BBa_K1606029_sequence (Version 1)

Description

we modernized m-cherry (BBa_J04450) part to report the absence of the gluten protein in a solution or in a dough that our updated Kumamax enzyme is inactive in the environment. When the absence of the protein of the gluten our repoter gene is expressed and give red light to environment.

Notes

atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacga

gttcgaaatcgaaggtgaaggtg

aaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggac

atcctgtccccgcagttccagta

cggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaat

gggaacgtgttatgaacttcgaa

gacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtgg

taccaacttcccgtccgacggtc

cggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggt

gaaatcaaaatgcgtctgaaact

gaaacca caa ctt ccagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaa

aaccgacatcaaactggac

atcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgctta

ataacgctgatagtgctagtgta

gatcgc

Source

It comes from genomic sequence and we add a sequence that square with the amino acid sequence of the gluten protein of PQLP amino acid sequence that is digested by Kumamax enzyme.

Other Properties

• igem#experience: None
• igem#status: Planning
• synbiohub#ownedBy: user/james
• synbiohub#ownedBy: user/myers
• synbiohub#topLevel: BBa_K1606029/1

Member of these Collections

• iGEM Parts Registry