BBa_B0034 1 BBa_B0034 RBS (Elowitz 1999) -- defines RBS efficiency 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 RBS based on Elowitz repressilator. false true _1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. <p>No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation23325 1 conserved range23325 1 5 8 BBa_B0012 1 BBa_B0012 TE from coliphageT7 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>. Released HQ 2013 Transcription terminator for the <i>E.coli</i> RNA polymerase. false false _1_ 0 24 7 In stock false <P> <P>Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG).<P> Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator. false Reshma Shetty annotation7020 1 BBa_B0012 range7020 1 1 41 annotation1686 1 T7 TE range1686 1 8 27 annotation1690 1 polya range1690 1 28 41 annotation1687 1 stop range1687 1 34 34 BBa_J23102 1 BBa_J23102 constitutive promoter family member 2006-08-03T11:00:00Z 2015-08-31T04:08:40Z isolated from library of promoters Released HQ 2013 replace later false true _52_ 0 483 95 In stock true N/A true John Anderson BBa_B0010 1 BBa_B0010 T1 from E. coli rrnB 2003-11-19T12:00:00Z 2015-08-31T04:07:20Z Transcriptional terminator consisting of a 64 bp stem-loop. false false _1_ 0 24 7 In stock false true Randy Rettberg annotation7018 1 BBa_B0010 range7018 1 1 80 annotation4184 1 stem_loop range4184 1 12 55 BBa_K346002 1 BBa_K346002 PmerT promoter (mercury-responsive) 2010-10-11T11:00:00Z 2015-07-14T12:33:51Z TTCCATATCGCTTGACTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCC Released HQ 2013 This part was isolated from Tn21. When regulated by its cognate transcription factor MerR, it will represent a dose-response manner in response to the concentration of mercury. false false _457_ 4206 4411 9 In stock true It was divergently regulated by MerR false Qianzhu Wu & Mei Chen annotation2085551 1 promoter range2085551 1 1 57 BBa_E1010 1 mRFP1 **highly** engineered mutant of red fluorescent protein from Discosoma striata (coral) 2004-07-27T11:00:00Z 2015-08-31T04:07:26Z Campbell et al., PNAS v99 p7877 <a href="http://www.pubmedcentral.nih.gov/articlerender.fcgi?tool=pubmed&pubmedid=12060735">URL</a> Released HQ 2013 monomeric RFP: Red Fluorescent Protein. Excitation peak: 584 nm Emission peak: 607 nm false false _11_1_ 0 52 7 In stock false TAATAA double stop codon added (DE). Four silent mutations made to remove three EcoRI sites and one PstI site: A28G, A76G, A349G, G337A. true Drew Endy annotation1014044 1 mrfp1 range1014044 1 1 675 annotation2214014 1 Help:Barcodes range2214014 1 682 706 BBa_K1613019 1 BBa_K1613019 It can detect methyl mercury. 2015-09-02T11:00:00Z 2015-11-12T09:16:56Z K1420002 k1420001 MerB can combine with methyl mercury and turns it into mercury ion. MerR can combine with mercury ion and further reaction can proceed successfully. true false _2030_ 26843 26841 9 false ttgacagctagctcagtcctaggtactgtgctagcTACTAGAGATGAAGCTCGCCCCATATATTTTAGAACGTCTCACTTCGGTCAATCGTACCAATGGTACTGCGGATCTCTTGGTCCCGCTACTGCGGGAACTCGCCAAGGGGCGTCCGGTTTCACGAACGACACTTGCCGGGATTCTCGACTGGCCCGCTGAGCGAGTGGCCGCCGTACTCGAACAGGCCACCAGTACCGAATATGACAAAGATGGGAACATCATCGGCTACGGCCTCACCTTGCGCGAGACTTCGTATGTCTTTGAAATTGACGACCGCCGTCTGTATGCCTGGTGCGCGCTGGACACCTTGATATTTCCGGCGCTGATCGGCCGTACAGCTCGCGTCTCATCGCATTGCGCTGCAACCGGAGCACCCGTTTCACTCACGGTTTCACCCAGCGAGATACAGGCTGTCGAACCTGCCGGCATGGCGGTGTCCTTGGTATTGCCGCAGGAAGCAGCCGACGTTCGTCAGTCCTTCTGTTGCCATGTACATTTCTTTGCATCTGTCCCGACGGCGGAAGACTGGGCCTCCAAGCATCAAGGATTGGAAGGATTGGCGATCGTCAGTGTCCACGAGGCTTTCGGCTTGGGCCAGGAGTTTAATCGACATCTGTTGCAGACCATGTCATCTAGGACACCGTGATACTAGAGAAAGAGGAGAAATACTAGAGTACTAGATGGAAAATAATTTGGAAAACCTGACCATTGGCGTTTTTGCCAAGGCGGCCGGGGTCAACGTGGAGACAATCCGCTTCTATCAGCGCAAGGGCCTGTTGCGGGAACCGGACAAGCCTTACGGCAGCATCCGCCGCTATGGGGAGGCGGACGTGGTTCGGGTGAAATTCGTGAAATCGGCACAGCGGCTGGGGTTCAGTCTGGACGAGATTGCCGAGCTGTTGCGGCTCGACGATGGCACCCACTGCGAGGAGGCCAGCAGCCTGGCCGAACACAAGCTCAAGGACGTGCGCGAGAAGATGGCCGACTTGGCGCGCATGGAAACCGTGCTGTCTGAACTCGTGTGCGCCTGCCATGCACGAAAGGGGAATGTTTCCTGCCCGTTGATCGCGTCACTACAGGGCGAAGCAGGCCTGGCAAGGTCAGCTATGCCTTAGTACTAGAGTTCCATATCGCTTGACTCCGTACATGAGTACGGAAGTAAGGTTACGCTATCCAATCCTACTAGAGTCACACAGGAAAGTACTAGAGATGGCTTCCTCCGAAGACGTTATCAAAGAGTTCATGCGTTTCAAAGTTCGTATGGAAGGTTCCGTTAACGGTCACGAGTTCGAAATCGAAGGTGAAGGTGAAGGTCGTCCGTACGAAGGTACCCAGACCGCTAAACTGAAAGTTACCAAAGGTGGTCCGCTGCCGTTCGCTTGGGACATCCTGTCCCCGCAGTTCCAGTACGGTTCCAAAGCTTACGTTAAACACCCGGCTGACATCCCGGACTACCTGAAACTGTCCTTCCCGGAAGGTTTCAAATGGGAACGTGTTATGAACTTCGAAGACGGTGGTGTTGTTACCGTTACCCAGGACTCCTCCCTGCAAGACGGTGAGTTCATCTACAAAGTTAAACTGCGTGGTACCAACTTCCCGTCCGACGGTCCGGTTATGCAGAAAAAAACCATGGGTTGGGAAGCTTCCACCGAACGTATGTACCCGGAAGACGGTGCTCTGAAAGGTGAAATCAAAATGCGTCTGAAACTGAAAGACGGTGGTCACTACGACGCTGAAGTTAAAACCACCTACATGGCTAAAAAACCGGTTCAGCTGCCGGGTGCTTACAAAACCGACATCAAACTGGACATCACCTCCCACAACGAAGACTACACCATCGTTGAACAGTACGAACGTGCTGAAGGTCGTCACTCCACCGGTGCTTAATAACGCTGATAGTGCTAGTGTAGATCGCTACTAGAGCCAGGCATCAAATAAAACGAAAGGCTCAGTCGAAAGACTGGGCCTTTCGTTTTATCTGTTGTTTGTCGGTGAACGCTCTCTACTAGAGTCACACTGGCTCACCTTCGGGTGGGCCTTTCTGCGTTTATA false HSU, CHIA-YANG component2443027 1 BBa_K1420004 component2443021 1 BBa_J23102 component2443025 1 BBa_K1420002 component2443023 1 BBa_B0034 component2443035 1 BBa_E1010 component2443031 1 BBa_B0032 component2443029 1 BBa_K346002 component2443042 1 BBa_B0015 annotation2443031 1 BBa_B0032 range2443031 1 1217 1229 annotation2443025 1 BBa_K1420002 range2443025 1 62 700 annotation2443027 1 BBa_K1420004 range2443027 1 709 1143 annotation2443029 1 BBa_K346002 range2443029 1 1152 1208 annotation2443021 1 BBa_J23102 range2443021 1 1 35 annotation2443023 1 BBa_B0034 range2443023 1 44 55 annotation2443035 1 BBa_E1010 range2443035 1 1236 1941 annotation2443042 1 BBa_B0015 range2443042 1 1950 2078 BBa_K1420004 1 BBa_K1420004 merR family transcriptional regulator, regulatory protein for mer operon 2014-10-08T11:00:00Z 2015-07-22T03:01:33Z Source organism: Serratia marcescens. Amplified from plasmid pDU1358, kindly donated by Anne O. Summers. merR, located upstream of the rest of the mer genes, serves to regulate the operon. The MerR protein contains a C-terminal effector binding region that recognizes ionic mercury. Upon binding it, the protein???s N-terminal helix-turn-helix DNA binding domain can bind the merR promoter region to induce transcription of the operon. The effector binding region of the protein can vary allowing for great diversity in merR-like promoters that can respond to a variety of heavy metals as well as antibiotics and oxidative stress. false false _1798_ 4206 17872 9 It's complicated false N/A false Stephen C. Heinsch annotation2422563 1 merR range2422563 1 1 435 BBa_B0032 1 BBa_B0032 RBS.3 (medium) -- derivative of BBa_0030 2003-01-31T12:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Weak1 RBS based on Ron Weiss thesis. Strength is considered relative to <bb_part>BBa_B0030</bb_part>, <bb_part>BBa_B0031</bb_part>, <bb_part>BBa_B0033</bb_part>. false true _41_44_48_46_1_ 0 24 7 In stock false Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix (&quot;RBS-2&quot; in figure 4-14 of thesis). <P> Contact info for this part: <a href="mailto:(bchow@media.mit.edu)">Brian Chow</a> true Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003. annotation1709 1 RBS-3\Weak range1709 1 1 13 annotation7027 1 BBa_B0032 range7027 1 1 13 annotation1710 1 RBS range1710 1 7 10 BBa_B0015 1 BBa_B0015 double terminator (B0010-B0012) 2003-07-16T11:00:00Z 2015-08-31T04:07:20Z Released HQ 2013 Double terminator consisting of BBa_B0010 and BBa_B0012 false true _1_ 0 24 7 In stock false true Reshma Shetty component1916610 1 BBa_B0010 component1916612 1 BBa_B0012 annotation1916612 1 BBa_B0012 range1916612 1 89 129 annotation1916610 1 BBa_B0010 range1916610 1 1 80 BBa_K1420002 1 BBa_K1420002 merB, Organomercurial Lyase from Serratia marcescens 2014-10-08T11:00:00Z 2015-07-22T02:52:43Z Amplified from pDU1358, kindly donated by Anne O. Summers. Demethylates organomerury. false false _1798_ 4206 17872 9 In stock false N/A false Stephen C. Heinsch annotation2407616 1 merB range2407616 1 1 639 BBa_K346002_sequence 1 ttccatatcgcttgactccgtacatgagtacggaagtaaggttacgctatccaatcc BBa_B0010_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc BBa_K1613019_sequence 1 ttgacagctagctcagtcctaggtactgtgctagctactagagaaagaggagaaatactagatgaagctcgccccatatattttagaacgtctcacttcggtcaatcgtaccaatggtactgcggatctcttggtcccgctactgcgggaactcgccaaggggcgtccggtttcacgaacgacacttgccgggattctcgactggcccgctgagcgagtggccgccgtactcgaacaggccaccagtaccgaatatgacaaagatgggaacatcatcggctacggcctcaccttgcgcgagacttcgtatgtctttgaaattgacgaccgccgtctgtatgcctggtgcgcgctggacaccttgatatttccggcgctgatcggccgtacagctcgcgtctcatcgcattgcgctgcaaccggagcacccgtttcactcacggtttcacccagcgagatacaggctgtcgaacctgccggcatggcggtgtccttggtattgccgcaggaagcagccgacgttcgtcagtccttctgttgccatgtacatttctttgcatctgtcccgacggcggaagactgggcctccaagcatcaaggattggaaggattggcgatcgtcagtgtccacgaggctttcggcttgggccaggagtttaatcgacatctgttgcagaccatgtcatctaggacaccgtgatactagagctacaccgcgtcggcaccacgcggctctttctccccttgcagcgaagcaatcagcgggcaagaaacgttcccttgccgcgcatggcaggcgaacacaagttcggatagcacggtttccatgcgcgccaggtcggtcattttttcgcgcacgtcctgaagcttgtgctcggccaggctgctggcttcctcgcagtgggtgccgtcatccagcctcagcagctctgcgatctcgtcgaggctgaatccgagccgctgggctgatttcacgaagcgcacccgcgtcacatccgcctcgccatagcggcggatgctgccatagggcttgtccggctccggcaacaagcccttgcgctgatagaaccggattgtttccacgttgaccccggccgccttggcgaaaacgccaatagtcagattctccaaatttttttccattactagagttccatatcgcttgactccgtacatgagtacggaagtaaggttacgctatccaatcctactagagtcacacaggaaagtactagatggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata BBa_K1420004_sequence 1 ctacaccgcgtcggcaccacgcggctctttctccccttgcagcgaagcaatcagcgggcaagaaacgttcccttgccgcgcatggcaggcgaacacaagttcggatagcacggtttccatgcgcgccaggtcggtcattttttcgcgcacgtcctgaagcttgtgctcggccaggctgctggcttcctcgcagtgggtgccgtcatccagcctcagcagctctgcgatctcgtcgaggctgaatccgagccgctgggctgatttcacgaagcgcacccgcgtcacatccgcctcgccatagcggcggatgctgccatagggcttgtccggctccggcaacaagcccttgcgctgatagaaccggattgtttccacgttgaccccggccgccttggcgaaaacgccaatagtcagattctccaaatttttttccat BBa_B0034_sequence 1 aaagaggagaaa BBa_K1420002_sequence 1 atgaagctcgccccatatattttagaacgtctcacttcggtcaatcgtaccaatggtactgcggatctcttggtcccgctactgcgggaactcgccaaggggcgtccggtttcacgaacgacacttgccgggattctcgactggcccgctgagcgagtggccgccgtactcgaacaggccaccagtaccgaatatgacaaagatgggaacatcatcggctacggcctcaccttgcgcgagacttcgtatgtctttgaaattgacgaccgccgtctgtatgcctggtgcgcgctggacaccttgatatttccggcgctgatcggccgtacagctcgcgtctcatcgcattgcgctgcaaccggagcacccgtttcactcacggtttcacccagcgagatacaggctgtcgaacctgccggcatggcggtgtccttggtattgccgcaggaagcagccgacgttcgtcagtccttctgttgccatgtacatttctttgcatctgtcccgacggcggaagactgggcctccaagcatcaaggattggaaggattggcgatcgtcagtgtccacgaggctttcggcttgggccaggagtttaatcgacatctgttgcagaccatgtcatctaggacaccgtga BBa_E1010_sequence 1 atggcttcctccgaagacgttatcaaagagttcatgcgtttcaaagttcgtatggaaggttccgttaacggtcacgagttcgaaatcgaaggtgaaggtgaaggtcgtccgtacgaaggtacccagaccgctaaactgaaagttaccaaaggtggtccgctgccgttcgcttgggacatcctgtccccgcagttccagtacggttccaaagcttacgttaaacacccggctgacatcccggactacctgaaactgtccttcccggaaggtttcaaatgggaacgtgttatgaacttcgaagacggtggtgttgttaccgttacccaggactcctccctgcaagacggtgagttcatctacaaagttaaactgcgtggtaccaacttcccgtccgacggtccggttatgcagaaaaaaaccatgggttgggaagcttccaccgaacgtatgtacccggaagacggtgctctgaaaggtgaaatcaaaatgcgtctgaaactgaaagacggtggtcactacgacgctgaagttaaaaccacctacatggctaaaaaaccggttcagctgccgggtgcttacaaaaccgacatcaaactggacatcacctcccacaacgaagactacaccatcgttgaacagtacgaacgtgctgaaggtcgtcactccaccggtgcttaataacgctgatagtgctagtgtagatcgc BBa_B0032_sequence 1 tcacacaggaaag BBa_J23102_sequence 1 ttgacagctagctcagtcctaggtactgtgctagc BBa_B0012_sequence 1 tcacactggctcaccttcgggtgggcctttctgcgtttata BBa_B0015_sequence 1 ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z