LOCUS       BBa_K1615013            2949 bp    DNA     linear       14-May-2024
DEFINITION  MorA fused to CBDcenA+Linker in RFC25 driven by LacI promoter
ACCESSION   BBa_K1615013
VERSION     BBa_K1615013.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     misc_feature    1645..1647
                     /label=start
     misc_feature    1639..1653
                     /label=BBa_K1319106
     CDS             1654..2535
                     /label=Morphine-6-dehydrogenase
     CDS             1654..2535
                     /label=BBa_K1615000
     misc_feature    2536..2541
                     /label=Scar 25
     misc_feature    2536..2541
                     /label=BBa_B0105
     CDS             2860..2949
                     /label=Linker: Pro-Thr box
     CDS             2542..2859
                     /label=CBDCenA
     CDS             2542..2949
                     /label=BBa_K1321339
     misc_feature    1654..2949
                     /label=BBa_K1615003
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaata ctagatggcc ggcgcaggta aaagtccgct gattaatctg
     1681 aataatggcg ttaaaatgcc tgcactgggt ctgggtgttt ttgcagcaag cgcagaagaa
     1741 accgcaagcg caattgcaag cgccattagc agcggttatc gtctgattga taccgcacgt
     1801 agctataaca atgaagcaca ggttggtgaa ggtattcgta atagcgttga tcgtgcagaa
     1861 atgtttgtta ccaccaaact gttcaattgc gattatggtt atgaacgtgc actgcgtgca
     1921 tttgatgaaa gcctgggtcg tctgggcctg gattatgttg atctgtatct gctgcattgg
     1981 ccgaccaaag attggaatgc aaccattcag agctggaaag cagcagaaaa aattctgggt
     2041 gatggtcgtg cacgtgccat tggtgtttgt aattttctgg aagatcagct ggatgaactg
     2101 attgcagcat cagatgttgt tccggcagtt aatcagatcg aactgcatcc gtattttgca
     2161 cagaaacctc tgctggcaaa aaatcgtgcc ctgggtattg ttaccgaagc atggtcaccg
     2221 attggttgtc atcagcgttg gcaggatggt gataatcatg gtggtcgtaa acatccgctg
     2281 accgatccgg ttattaccac cattgccgaa gcacatggtc gtagcgcagc ccaggttatt
     2341 ctgcgttggc attttcagaa tgatgttgtt gcaattccga aaagcgttaa tccggaacgt
     2401 attgccaaaa acattgatgt gtttgatttt gccctgtccg atgccgaaat ggcacagctg
     2461 gacgagctgg atacaggtgt tcgtattggt ccggatccgc gtgatgttga taccagcagc
     2521 tttgcagaat ttgtgaccgg cgcgccgggt tgccgtgttg actacgcggt taccaaccag
     2581 tggccgggtg gtttcggtgc gaacgttacc atcaccaacc tgggtgaccc ggtttcttct
     2641 tggaaactgg actggaccta caccgcgggt cagcgtatcc agcagctgtg gaacggtacc
     2701 gcgtctacca acggtggtca ggtttctgtt acctctctgc cgtggaacgg ttctatcccg
     2761 actggtggta ccgcgtcttt cggtttcaac ggttcttggg cgggttctaa cccgaccccg
     2821 gcgtctttct ctctgaacgg taccacctgc acgggtaccc cgacgacctc gccaactcct
     2881 acgccgaccc caaccacgcc gactcctaca ccgacgccca ccccaacgcc tactccgacg
     2941 gtcaccccc
//