LOCUS       BBa_K1615061            2946 bp    DNA     linear       18-May-2024
DEFINITION  CBDcex fused to Heroin Esterase driven by LacI promoter
ACCESSION   BBa_K1615061
VERSION     BBa_K1615061.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     misc_feature    1639..1644
                     /label=Scar 25
     misc_feature    1639..1644
                     /label=BBa_B0105
     CDS             1645..1971
                     /label=BBa_K1321003
     misc_feature    1972..1977
                     /label=Scar 25
     misc_feature    1972..1977
                     /label=BBa_B0105
     CDS             1978..2946
                     /label=BBa_K1615045
     misc_feature    1645..2946
                     /label=BBa_K1615051
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaaac cggcggtccg gccgggtgcc aggtgctgtg gggcgtcaac
     1681 cagtggaaca ccggcttcac cgcgaacgtc accgtgaaga acacgtcctc cgctccggta
     1741 gacggctgga cgctcacgtt cagcttcccg tccggccagc aggtcaccca ggcgtggagc
     1801 tcgacggtca cgcagtccgg ctcggccgtg acggtccgca acgccccgtg gaacggctcg
     1861 atcccggcgg gcggcaccgc gcagttcggc ttcaacggct cgcacacggg caccaacgcc
     1921 gcgccgacgg cgttctcgct caacggcacg ccctgcacgg tcggcggcag caccggcatg
     1981 accacctttc cgaccctgga tccggaactg gcagcagcac tgaccatgct gccgaaagtt
     2041 gattttgccg atctgccgaa tgcacgtgcc acctataatg cactgattgg tgcaatgctg
     2101 gcggatctgc cgtttgatgg tgttagcctg cgtgaactga gcgcaccggg tctggatggt
     2161 gatccggaag ttaccattcg tttttttaca ccggataata ccgcaggtcc ggttccggtt
     2221 ctgctgtgga ttcatggtgg tggttttgca attggcaccg cagaaagcag cgatccgttt
     2281 tgtgttgaag ttgcacgtga actgggtttt gcagttgcaa atgttgaata tcgtctggca
     2341 ccggaaacca gctttccggg tccggttaat gattgttatg cagcactgct gtatattcat
     2401 gcacatgccg aagaactggg tgttgatccg agccgtattg cagttggtgg tcagagtgcc
     2461 ggtggtggtc tggcagccgg tacagttctg aaagcacgtg atgaaggtgt tgttccggtt
     2521 gcatttcagt ttctggaaat tcctgaactg gatgatcgcc tggcaaccgt tagcatgacc
     2581 aattttgttg atacaccgct gtggcatcgt ccgaatgcaa ttctgagctg gaaatattac
     2641 ctgggtgaaa gctatagcgg tccggaagat ccggatgtga gcatttatgc cgcaccgagc
     2701 cgtgcaaccg atctgacagg tctgcctccg acctatctga gcaccatgga actggatccg
     2761 ctgcgtgatg agggtctgga atatgcactg cgtctgctgc atgccggtgt tagcgttgaa
     2821 ctgcatagtt ttccgggtac atttcatggt agcgcactgg ttgccaccgc agcagttagc
     2881 gaacgtggtg cagcagaagc cctgacagcc ctgcgtcgtg gtctgcgtag cctgagtccg
     2941 gttagc
//