LOCUS       BBa_K1615063            2928 bp    DNA     linear       18-May-2024
DEFINITION  CBDclos fused to Heroin Esterase driven by LacI promoter
ACCESSION   BBa_K1615063
VERSION     BBa_K1615063.1  
FEATURES             Location/Qualifiers
     misc_feature    18..324
                     /label=f1 origin
     misc_feature    1..455
                     /label=BBa_K314110
     misc_feature    464..1555
                     /label=BBa_K314111
     misc_feature    1566..1582
                     /label=lac O1
     promoter        1583..1588
                     /label=-35
     misc_feature    1589..1605
                     /label=lac O1
     promoter        1606..1611
                     /label=-10
     misc_feature    1564..1617
                     /label=BBa_R0011
     promoter        1564..1617
                     /label=BBa_R0011
     misc_feature    1631..1634
                     /label=conserved
     RBS             1627..1638
                     /label=BBa_B0034
     misc_feature    1..1638
                     /label=BBa_K314103
     misc_feature    1645..1647
                     /label=start
     misc_feature    1639..1653
                     /label=BBa_K1319106
     CDS             1654..1953
                     /label=BBa_K1321002
     misc_feature    1954..1959
                     /label=Scar 25
     misc_feature    1954..1959
                     /label=BBa_B0105
     CDS             1960..2928
                     /label=BBa_K1615045
     misc_feature    1654..2928
                     /label=BBa_K1615053
ORIGIN
        1 acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg
       61 ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca
      121 cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta
      181 gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc
      241 catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg
      301 gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct tttgatttat
      361 aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta
      421 acgcgaattt taacaaaata ttaacgttta caatttacta gaggtggtga atgtgaaacc
      481 agtaacgtta tacgatgtcg cagagtatgc cggtgtctct tatcagaccg tttcccgcgt
      541 ggtgaaccag gccagccacg tttctgcgaa aacgcgggaa aaagtggaag cggcgatggc
      601 ggagctgaat tacattccca accgcgtggc acaacaactg gcgggcaaac agtcgttgct
      661 gattggcgtt gccacctcca gtctggccct gcacgcgccg tcgcaaattg tcgcggcgat
      721 taaatctcgc gccgatcaac tgggtgccag cgtggtggtg tcgatggtag aacgaagcgg
      781 cgtcgaagcc tgtaaagcgg cggtgcacaa tcttctcgcg caacgcgtca gtgggctgat
      841 cattaactat ccgctggatg accaggatgc cattgctgtg gaagctgcct gcactaatgt
      901 tccggcgtta tttcttgatg tctctgacca gacacccatc aacagtatta ttttctccca
      961 tgaagacggt acgcgactgg gcgtggagca tctggtcgca ttgggtcacc agcaaatcgc
     1021 gctgttagcg ggcccattaa gttctgtctc ggcgcgtctg cgtctggctg gctggcataa
     1081 atatctcact cgcaatcaaa ttcagccgat agcggaacgg gaaggcgact ggagtgccat
     1141 gtccggtttt caacaaacca tgcaaatgct gaatgagggc atcgttccca ctgcgatgct
     1201 ggttgccaac gatcagatgg cgctgggcgc aatgcgcgcc attaccgagt ccgggctgcg
     1261 cgttggtgcg gacatctcgg tagtgggata cgacgatacc gaagacagct catgttatat
     1321 cccgccgtta accaccatca aacaggattt tcgcctgctg gggcaaacca gcgtggaccg
     1381 cttgctgcaa ctctctcagg gccaggcggt gaagggcaat cagctgttgc ccgtctcact
     1441 ggtgaaaaga aaaaccaccc tggcgcccaa tacgcaaacc gcctctcccc gcgcgttggc
     1501 cgattcatta atgcagctgg cacgacaggt ttcccgactg gaaagcgggc agtgatacta
     1561 gagaattgtg agcggataac aattgacatt gtgagcggat aacaagatac tgagcacata
     1621 ctagagaaag aggagaaata ctagatggcc ggctcatcaa tgtcagttga attttacaac
     1681 tctaacaaat cagcacaaac aaactcaatt acaccaataa tcaaaattac taacacgtct
     1741 gacagtgatt taaatttaaa tgacgtaaaa gttagatatt attacacaag tgatggtaca
     1801 caaggacaaa ctttctggtg tgaccatgct ggtgcattat taggaaatag ctatgttgat
     1861 aacactagca aagtgacagc aaacttcgtt aaagaaacag caagcccaac atcaacctat
     1921 gatacatatg ttgaatttgg atttgcaggc agcaccggca tgaccacctt tccgaccctg
     1981 gatccggaac tggcagcagc actgaccatg ctgccgaaag ttgattttgc cgatctgccg
     2041 aatgcacgtg ccacctataa tgcactgatt ggtgcaatgc tggcggatct gccgtttgat
     2101 ggtgttagcc tgcgtgaact gagcgcaccg ggtctggatg gtgatccgga agttaccatt
     2161 cgttttttta caccggataa taccgcaggt ccggttccgg ttctgctgtg gattcatggt
     2221 ggtggttttg caattggcac cgcagaaagc agcgatccgt tttgtgttga agttgcacgt
     2281 gaactgggtt ttgcagttgc aaatgttgaa tatcgtctgg caccggaaac cagctttccg
     2341 ggtccggtta atgattgtta tgcagcactg ctgtatattc atgcacatgc cgaagaactg
     2401 ggtgttgatc cgagccgtat tgcagttggt ggtcagagtg ccggtggtgg tctggcagcc
     2461 ggtacagttc tgaaagcacg tgatgaaggt gttgttccgg ttgcatttca gtttctggaa
     2521 attcctgaac tggatgatcg cctggcaacc gttagcatga ccaattttgt tgatacaccg
     2581 ctgtggcatc gtccgaatgc aattctgagc tggaaatatt acctgggtga aagctatagc
     2641 ggtccggaag atccggatgt gagcatttat gccgcaccga gccgtgcaac cgatctgaca
     2701 ggtctgcctc cgacctatct gagcaccatg gaactggatc cgctgcgtga tgagggtctg
     2761 gaatatgcac tgcgtctgct gcatgccggt gttagcgttg aactgcatag ttttccgggt
     2821 acatttcatg gtagcgcact ggttgccacc gcagcagtta gcgaacgtgg tgcagcagaa
     2881 gccctgacag ccctgcgtcg tggtctgcgt agcctgagtc cggttagc
//