BBa_K1615089 1 mRFP mRFP RFC25 2015-08-30T11:00:00Z 2016-01-25T11:25:49Z mRFP highly engineered variant originally from Discosoma striata. Highly engineered monomeric mutant of RFP. Improved version of part BBa_E1010 to remove illegal AgeI sites and add RFC25 prefix and suffix. false false _2032_ 4206 25547 9 false Codon optimised for E. coli. RFC 25 compatible. false Brooke Rothschild-Mancinelli annotation2440569 1 mRFP range2440569 1 1 675 BBa_K1321002 1 BBa_K1321002 Re-entry of BBa_K863111, Cellulose Binding Domain CBDclos in rfc25 2014-10-09T11:00:00Z 2015-06-17T12:35:41Z This is a direct copy of BBa_K863111 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part. This part is a copy of BBa_K863111 (Cellulose binding domain of C. cellulovorans cellulose binding protein gene (Freiburg-Standard)) and was created only as a registry entry, removing the atg-NgoMIV parts at the front and the AgeI-taa basepairs from the sequence so that the registry does not flag it as rfc25 incompatible, and it enables us to enter fusion proteins with this part as automatic composite parts in the method as described by: http://2009.igem.org/Team:Freiburg_bioware/general. false false _1696_ 4206 20780 9 Not in stock false This is a direct copy of BBa_K863111 with slight modifications to the beginning and end of the sequence for the purposes of allowing composite parts to be added correctly using this part. false Xenia Spencer-Milnes BBa_K1615098 1 BBa_K1615098 mRFP fused to CBDclos in RFC25 2015-08-30T11:00:00Z 2015-09-18T12:07:20Z mRFP highly engineered variant originally from Discosoma striata. CBDclos from Clostridium cellulovorans. mRFP fused N terminally to CBDclos. false false _2032_ 25547 25547 9 false Fusion done using RFC25. false Brooke Rothschild-Mancinelli component2473469 1 BBa_B0105 component2473471 1 BBa_K1615089 component2473467 1 BBa_K1321002 annotation2473469 1 BBa_B0105 range2473469 1 301 306 annotation2473471 1 BBa_K1615089 range2473471 1 307 981 annotation2473467 1 BBa_K1321002 range2473467 1 1 300 BBa_B0105 1 Scar 25 RFC 25 Scar Sequence 2009-10-14T11:00:00Z 2015-08-31T04:07:21Z BBF RFC 25 This is the scar produced by assembly using RFC 25. If you are assembling a composite part using RFC 25, you can insert this part and specify blunt assembly to get the desired sequence. false false _1_ 0 25 397 Not in stock false Simple DNA sequence false Randy Rettberg annotation2041621 1 Scar 25 range2041621 1 1 6 BBa_K1615089_sequence 1 atggcaagcagcgaagatgtgatcaaagaatttatgcgtttcaaagtgcgtatggaaggtagcgttaatggccatgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttcagtatggtagcaaagcatatgttaaacatccggcagatatcccggattatctgaaactgagctttccggaaggttttaaatgggaacgtgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcctgcaagatggtgaatttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaatgcgcctgaaactgaaagatggtggccattatgatgcagaagttaaaaccacctacatggccaaaaaacctgttcagctgcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagcacaggtgca BBa_B0105_sequence 1 accggc BBa_K1615098_sequence 1 tcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagcaccggcatggcaagcagcgaagatgtgatcaaagaatttatgcgtttcaaagtgcgtatggaaggtagcgttaatggccatgaatttgaaattgaaggtgaaggcgaaggtcgtccgtatgaaggcacccagaccgcaaaactgaaagttaccaaaggtggtccgctgccgtttgcatgggatattctgagtccgcagtttcagtatggtagcaaagcatatgttaaacatccggcagatatcccggattatctgaaactgagctttccggaaggttttaaatgggaacgtgtgatgaattttgaagatggtggtgttgttaccgttacccaggatagcagcctgcaagatggtgaatttatctacaaagttaaactgcgtggcaccaactttccgagtgatggtccggttatgcagaaaaaaaccatgggttgggaagcaagcaccgaacgtatgtatccggaagatggcgcactgaaaggtgaaatcaaaatgcgcctgaaactgaaagatggtggccattatgatgcagaagttaaaaccacctacatggccaaaaaacctgttcagctgcctggtgcatataaaaccgatattaaactggatatcaccagccacaacgaggattataccattgttgaacagtatgaacgtgcagaaggtcgccatagcacaggtgca BBa_K1321002_sequence 1 tcatcaatgtcagttgaattttacaactctaacaaatcagcacaaacaaactcaattacaccaataatcaaaattactaacacgtctgacagtgatttaaatttaaatgacgtaaaagttagatattattacacaagtgatggtacacaaggacaaactttctggtgtgaccatgctggtgcattattaggaaatagctatgttgataacactagcaaagtgacagcaaacttcgttaaagaaacagcaagcccaacatcaacctatgatacatatgttgaatttggatttgcaggcagc igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z