Source:
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brooke Rothschild-Mancinelli
Date created: 2015-08-30 11:00:00
Date modified: 2015-08-31 05:14:41
mRFP fused to CBDclos driven by LacI promoter
| Types | DnaRegion |
| Roles | engineered_region Composite |
| Sequences | BBa_K1615108_sequence (Version 1) |
Description
mRFP fused N terminally to CBDclos driven by the IPTG inducible PLlac 0-1 promoter.Notes
Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.Source
mRFP highly engineered variant originally from Discosoma striata. CBDclos from Clostridium cellulovorans. Lac expression cassette from iGEM10_Washington.| Access | Instance | Definition |
| public public public public public public | BBa_K314103 BBa_K1319106 BBa_K1615089 BBa_B0105 BBa_K1321002 BBa_K1615098 | BBa_K314103 Scar25 ATG mRFP Scar 25 BBa_K1321002 BBa_K1615098 |
| Sequence Annotation | Location | Component / Role(s) |
| BBa_K314103 BBa_K1319106 BBa_K1615089 BBa_B0105 BBa_K1321002 BBa_K1615098 | 1,1638 1639,1653 1654,2328 2329,2334 2335,2634 1654,2634 | BBa_K314103 Scar25 ATG mRFP Scar 25 BBa_K1321002 BBa_K1615098 |