BBa_K1615110

BBa_K1615110 Version 1

Component

Source:
http://parts.igem.org/Part:BBa_K1615110
Generated By: https://synbiohub.org/public/igem/igem2sbol/1
Created by: Brooke Rothschild-Mancinelli
Date created: 2015-08-30 11:00:00
Date modified: 2015-08-31 05:20:34

mRFP fused to Linker-CBDcipA-linker driven by LacI promoter



Types
DnaRegion

Roles
Composite

engineered_region

Sequences BBa_K1615110_sequence (Version 1)

Description

mRFP fused N terminally to CBDcipA with N and C terminal linkers driven by the IPTG inducible PLlac 0-1 promoter.

Notes

Fusion was performed using RFC10 standard due to illegal NgoMIV site in f1 origin.

Source

mRFP highly engineered variant originally from Discosoma striata. CBDcipA from Clostridium thermocellum. Lac expression cassette from iGEM10_Washington.

igem#status
Planning
 
synbiohub#ownedBy
user/james
 
synbiohub#ownedBy
user/myers
 
synbiohub#topLevel
BBa_K1615110/1